Research notes: Immunohistochemical observations in the ceca of chickens infected with Salmonella enteritidis phage type four

Desmidt, Maria; Ducatelle, Richard; Haesebrouck, Freddy

doi:10.1093/ps/77.1.73

Cited by 41 publications

(23 citation statements)

References 4 publications

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“…Chemical bursectomy has the disadvantage that cell types other than only B cells may be affected. Colonization of liver and spleen decreased over time in control as well as in bursectomized animals, indicating that other immune mechanisms play a role in systemic clearance of S. Enteritidis in chickens (Desmidt et al , 1998c). By contrast surgically bursectomzed chickens, in which only B cell maturation would be affected, showed clearance of S. Typhimurium from the intestine at the same rate as nonbursectomized birds (Beal et al , 2006), demonstrating that antibody response is not essential to gut clearance which poses a number of fundamental and interesting questions about the nature and anatomical site/location of immune clearance.…”

Section: Immunity To Salmonellamentioning

confidence: 97%

“…Clearance of S. Typhimurium infection in chickens correlates with high cell-mediated responses (delayed type hypersensitivity reaction) and not with high antibody levels (Lee et al , 1981;Ib 1983). A study of Desmidt et al (1998c) with S. Enteritidis infected, chemically bursectomized chickens showed increased faecal excretion and higher caecal Salmonella counts, while having normal counts in internal organs, indicative of a protective effect of IgA against intestinal colonization. Chemical bursectomy has the disadvantage that cell types other than only B cells may be affected.…”

Section: Immunity To Salmonellamentioning

confidence: 99%

“…Thus, after vaccination of one-day old chicks, production of significant amounts of specific antibody responses against Salmonella takes more than 10 days (Desmidt et al, 1998b). For infections which may occur before this time, such as those arising Colonisation-inhibition, or competitive exclusion, as it is more commonly known, can also be induced by the administration of normal gut flora preparations to newly hatched chicks.…”

Section: Colonisation-inhibitionmentioning

confidence: 99%

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Salmonella infections: immune and non-immune protection with vaccines

Barrow

2007

Avian Pathology

137

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Section: Immunity To Salmonellamentioning

confidence: 97%

Section: Immunity To Salmonellamentioning

confidence: 99%

Section: Colonisation-inhibitionmentioning

confidence: 99%

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Salmonella infections: immune and non-immune protection with vaccines

Barrow

2007

Avian Pathology

137

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“…Our infection model was based on the previous studies, which showed that orally introduced S. enteritidis had a rapid transit time through the intestine and established itself within the walls of the gut in more than 3 d [17,18] . 80 mice (age 9 wk, specific-pathogen-free) were purchased from the Animal Center of Sichuan University, China.…”

Section: Experimental Infection Of Micementioning

confidence: 99%

Quantitative studies of the regular distribution pattern for Salmonella enteritidis in the internal organs of mice after oral challenge by a specific real-time polymerase chain reaction

Deng

Cheng²,

Wang³

et al. 2008

WJG

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RESULTS:The results showed that the spleen was positive at 12 h post inoculation (PI), and the blood was at 14 h PI. The organism was detected in the liver and heart at 16 h PI, the pancreas was positive at 20 h PI, and the final organs to show positive results were the kidney and gallbladder at 22 h PI. The copy number of S. enteritidis DNA in each tissue reached a peak at 24-36 h PI, with the liver and spleen containing high concentrations of S. enteritidis , whereas the blood, heart, kidney, pancreas, and gallbladder had low concentrations.S. enteritidis populations began to decrease and were not detectable at 3 d PI, but were still present up to 12 d PI in the gallbladder, 2 wk for the liver, and 3 wk for the spleen without causing apparent symptoms. CONCLUSION:The results provided significant data for understanding the life cycle of S. enteritidis in the internal organs, and showed that the liver and spleen may be the primary sites for setting itself up as a commensal over a long time after oral challenge. Interestingly, it may be the first time reported that the gallbladder is a site of carriage for S. METHODS:Assays based on the serovar-specific DNA sequence of S. enteritidis from GenBank, and a serovarspecific real-time, fluorescence-based quantitative polymerase chain reaction (FQ-PCR) were developed for the detection of S. enteritidis . We used this assay to detect genomic DNA of S. enteritidis in the blood and the internal organs, including heart, liver, spleen, kidney, pancreas, and gallbladder, from mice after oral challenge at different time points respectively.

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“…Enteritidis results in attachment and entering of bacteria to the epithelial cells of intestinal villi (Asheg et al, 2003). After lysis of the host cells, bacteria are found in the lamina propria lying free or inside macrophage-like cells (Desmidt et al, 1998).…”

mentioning

confidence: 99%

Effect of preventive application of Enterococcus faecium EF55 on intestinal mucosa during salmonellosis in chickens

Herich¹,

Kokinčáková²,

Lauková³

et al. 2010

Czech J. Anim. Sci.

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In the present study the effect of preventive application of Enterococcus faecium EF 55 on the intestinal mucosa was evaluated in experimentally infected chickens with Salmonella enterica subsp. Enteritidis. A total of 120, one-day-old Salmonella-free chickens of Isa Brown hybrid were divided into 4 groups. The chickens in groups E and ES were perorally inoculated with E. faecium EF55 in a dose of 1 × 109 CFU/ml for 7 consecutive days. Placebo was applied to birds in control group C and group S during the first 7 days of life. At the age of 8 days chickens in groups ES and S were perorally infected with S. enterica subsp. Enteritidis phage type 4 in a dose of 1 × 108 CFU/ml. In birds treated with E. faecium EF 55 (group ES) a decreased number of Salmonella spp. positive individuals was recorded from 28.5% 2 days post infection (p.i.) to 10% 14 days p.i. when the difference between group ES and group with the application of Salmonella Enteritidis alone (group S) was significant (P < 0.01). On the contrary, in birds of group S the percentage of Salmonella spp. positive animals showed no constant changes. It increased from 12.5% 2 days p.i. to 37.5% 4 days p.i. The maximum of positive samples 83.3% was found 14 days p.i. The application of E. faecium EF55 reduced colonisation of caeca and minimized translocation of salmonellae into the liver and spleen. Two days p.i. the shortest villi in the jejunum were observed in group S – 1 266.2 µm, when compared to group E with the highest jejunal villi – 1 605 µm (P < 0.05). The growth of the villi was observed 14 days p.i. in all groups except group S. The early exposition of chickens to E. faecium EF55 led to more rapid development of intestinal villi when compared to the untreated control (P < 0.05). Reduced colonisation of the intestinal tract by salmonellae in birds treated with E. faecium EF 55 also preserved the microenvironment of the intestine from harmful effects of the pathogen.

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Research notes: Immunohistochemical observations in the ceca of chickens infected with Salmonella enteritidis phage type four

Cited by 41 publications

References 4 publications

Salmonella infections: immune and non-immune protection with vaccines

Salmonella infections: immune and non-immune protection with vaccines

Quantitative studies of the regular distribution pattern for Salmonella enteritidis in the internal organs of mice after oral challenge by a specific real-time polymerase chain reaction

Effect of preventive application of Enterococcus faecium EF55 on intestinal mucosa during salmonellosis in chickens

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