Research of temperature control algorithm in PCR gene amplify instrument

Abstract

To achieve stable and accurate temperature control in PCR gene amplify instrument, A complex PID control algorithm, including Bang-Bang control and Fuzzy-PID control algorithm, was proposed in this paper to improve the control dynamical and stable ability. The hardware of PCR gene amplify instrument and PID controller and fuzzy logic reasoning are introduced in this paper. The process of PID controller, fuzzy logic PID controller and fuzzy logic velocity PID controller controls nonlinearity system are emulated respectively with MATLAB SIMULINK, so that the control result can be compared. Experiments in the self-made PCR gene amplify instrument manifest fuzzy logic velocity PID is the most stable and accurate temperature control algorithm.

Optimization of nonlinear controller design for thermal management in polymerase chain reaction amplification

“…Heating and cooling operation is managed by a thermoelectric module named Peltier. [2][3][4] The amplification of DNA samples is performed in Thermal Cycler through multiple temperature cycles, with each cycle consisting of three preset temperatures: high (typically 92°C-96°C), low (typically 45°C-65°C) and medium (typically 68°C-74°C). Then, this desirable temperature-time path is given to machine as an input by biologist, which will be tracked by Peltier thermal function.…”

“…7,8 So far, different methods have been employed to control Peltiers in these machines. 4,[7][8][9] Proportional-integral-derivative (PID) and switching PID controllers are the most widely used control structures designed in singlestage Peltier-based thermal devices. 10,11 Also, PID controller designs have been used for some multiple-stage Peltier devices, 11,12 which denote that separate linear controllers are used for each Peltier module without any coupling.…”

High Throughput SNP Detection System Based on Magnetic Nanoparticles Separation

“…Heat generated by current flowing in one direction was absorbed if the current was reversed. Through a complex control algorithm combining fuzzy incremental PID control with the Bang-Bang control, 24 this system can realize precise temperature control for the samples in a 96-well plate. Thus this system can complete the hybridization of nucleic acid, the amplification of target sequence and so on.…”