Research on nanoparticles in human perfused placenta: State of the art and perspectives

Aengenheister, Leonie; Favaro, Rodolfo R.; Morales-Prieto, Diana M.; Furer, Lea A; Gruber, Michael; Wadsack, Christian; Markert, Udo R.; Buerki-Thurnherr, Tina

doi:10.1016/j.placenta.2020.12.014

Cited by 35 publications

(32 citation statements)

References 73 publications

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“…But carbon particle concentrations did not significantly increase in the fetal circulation after 6 h of perfusion, despite (i) a three orders of magnitude higher particle concentration in the maternal perfusate and (ii) the thinnest state of the actual cellular barrier at term pregnancy [ 50 , 51 ]. Therefore, our study suggests that the transfer of carbon particles across the human placenta is possible yet highly limited (as compared to other ex vivo particle translocation studies with particles in similar size ranges but different material [ 52 ]). However, only short exposure times (6 h) (due to tissue degradation [ 53 ]) were studied, and low deposition of particles in the perfusion system as well as variations between individual measurements are possibly masking low particle counts.…”

Section: Discussionmentioning

confidence: 95%

“…However, only short exposure times (6 h) (due to tissue degradation [ 53 ]) were studied, and low deposition of particles in the perfusion system as well as variations between individual measurements are possibly masking low particle counts. In addition, only a limited amount of perfusions (two control perfusions and four DEPs perfusions) was performed due to the high complexity and low success rate of this method [ 52 ], nevertheless, the obtained results were highly consistent even from this low number of independent experiments. Further verification of realistic direct chronic exposure of the fetus to combustion-derived particles could be pursued from human cord blood samples to corroborate the maternal–fetal transfer of carbon particles further.…”

Section: Discussionmentioning

confidence: 99%

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Label-free detection of uptake, accumulation, and translocation of diesel exhaust particles in ex vivo perfused human placenta

et al. 2021

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Background Pregnant women and developing fetuses comprise a particularly vulnerable population as multiple studies have shown associations between prenatal air pollution exposure and adverse pregnancy outcomes. However, the mechanisms underlying the observed developmental toxicity are mostly unknown, in particular, if pollution particles can cross the human placenta to reach the fetal circulation. Results Here, we investigated the accumulation and translocation of diesel exhaust particles (DEPs), as a model particle for combustion-derived pollution, in human perfused placentae using label-free detection by femtosecond pulsed laser illumination. The results do not reveal a significant particle transfer across term placentae within 6 h of perfusion. However, DEPs accumulate in placental tissue, especially in the syncytiotrophoblast layer that mediates a wealth of essential functions to support and maintain a successful pregnancy. Furthermore, DEPs are found in placental macrophages and fetal endothelial cells, showing that some particles can overcome the syncytiotrophoblasts to reach the fetal capillaries. Few particles are also observed inside fetal microvessels. Conclusions Overall, we show that DEPs accumulate in key cell types of the placental tissue and can cross the human placenta, although in limited amounts. These findings are crucial for risk assessment and protection of pregnant women and highlight the urgent need for further research on the direct and indirect placenta-mediated developmental toxicity of ambient particulates.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Label-free detection of uptake, accumulation, and translocation of diesel exhaust particles in ex vivo perfused human placenta

et al. 2021

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“…Figure 2 shows the basic tissue structure of a full-term placenta, with special reference to the mesenchymal tissue compartment from which stromal cells could be isolated. In view of the vast availability of the disposable human full-term placentae, many studies focused on this source for isolation of MSCs [ 174 , 175 , 176 , 177 , 178 , 179 ].…”

Section: Application Of Hpscs From Mature Placentae For Tissue Regeneration and Immune Modulationmentioning

confidence: 99%

Allogenic Use of Human Placenta-Derived Stromal Cells as a Highly Active Subtype of Mesenchymal Stromal Cells for Cell-Based Therapies

Gorodetsky

Aicher

2021

IJMS

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The application of mesenchymal stromal cells (MSCs) from different sources, including bone marrow (BM, bmMSCs), adipose tissue (atMSCs), and human term placenta (hPSCs) has been proposed for various clinical purposes. Accumulated evidence suggests that the activity of the different MSCs is indirect and associated with paracrine release of pro-regenerative and anti-inflammatory factors. A major limitation of bmMSCs-based treatment for autologous application is the limited yield of cells harvested from BM and the invasiveness of the procedure. Similar effects of autologous and allogeneic MSCs isolated from various other tissues were reported. The easily available fresh human placenta seems to represent a preferred source for harvesting abundant numbers of human hPSCs for allogenic use. Cells derived from the neonate tissues of the placenta (f-hPSC) can undergo extended expansion with a low risk of senescence. The low expression of HLA class I and II on f-hPSCs reduces the risk of rejection in allogeneic or xenogeneic applications in normal immunocompetent hosts. The main advantage of hPSCs-based therapies seems to lie in the secretion of a wide range of pro-regenerative and anti-inflammatory factors. This renders hPSCs as a very competent cell for therapy in humans or animal models. This review summarizes the therapeutic potential of allogeneic applications of f-hPSCs, with reference to their indirect pro-regenerative and anti-inflammatory effects and discusses clinical feasibility studies.

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“…The use of nanotherapy in pregnant women is being studied [78], considering that the treatments used must not affect the proper functioning of the placenta and the development of the fetus. Therefore, data on the bypassing of the placental barrier by NPs obtained in part by ex vivo cotyledon perfusion are essential for the development of these therapies [79]. Several air pollution NPs have been detected in human placentas [80] and some of them, such as carbon black, TiO 2 and silver, are known to cross the human placental barrier [81,82].…”

Section: Impact Of Nanoparticles During Pregnancymentioning

confidence: 99%

On Placental Toxicology Studies and Cerium Dioxide Nanoparticles

Deval

Boland

Fournier

et al. 2021

IJMS

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The human placenta is a transient organ essential for pregnancy maintenance, fetal development and growth. It has several functions, including that of a selective barrier against pathogens and xenobiotics from maternal blood. However, some pollutants can accumulate in the placenta or pass through with possible repercussions on pregnancy outcomes. Cerium dioxide nanoparticles (CeO2 NPs), also termed nanoceria, are an emerging pollutant whose impact on pregnancy is starting to be defined. CeO2 NPs are already used in different fields for industrial and commercial applications and have even been proposed for some biomedical applications. Since 2010, nanoceria have been subject to priority monitoring by the Organization for Economic Co-operation and Development in order to assess their toxicity. This review aims to summarize the current methods and models used for toxicology studies on the placental barrier, from the basic ones to the very latest, as well as to overview the most recent knowledge of the impact of CeO2 NPs on human health, and more specifically during the sensitive window of pregnancy. Further research is needed to highlight the relationship between environmental exposure to CeO2 and placental dysfunction with its implications for pregnancy outcome.

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Research on nanoparticles in human perfused placenta: State of the art and perspectives

Cited by 35 publications

References 73 publications

Label-free detection of uptake, accumulation, and translocation of diesel exhaust particles in ex vivo perfused human placenta

Label-free detection of uptake, accumulation, and translocation of diesel exhaust particles in ex vivo perfused human placenta

Allogenic Use of Human Placenta-Derived Stromal Cells as a Highly Active Subtype of Mesenchymal Stromal Cells for Cell-Based Therapies

On Placental Toxicology Studies and Cerium Dioxide Nanoparticles

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