In this work, the results of plasma microwave transmission diagnosis were analyzed. According to the attenuation and phase shift of the electromagnetic wave propagating in the plasma, the electron density and collision frequency of the plasma can be diagnosed. Since part of the electromagnetic wave is reflected or diffracted when propagating in the plasma, and is not absorbed by the plasma, and this part of the attenuation is still included in the measured attenuation, the attenuation is distorted. Therefore, a curve fitting method is proposed to remove the attenuation caused by the plasma reflection, thereby improving the accuracy of the diagnosis of the collision frequency. The calibration effect of this method on plasmas with different electron densities and collision frequencies is analyzed, and a diagnostic frequency band with good calibration results is given. The curve fitting method is verified by experiment and simulation. After adopting the newly proposed method, the diagnosis accuracy of collision frequency can be increased by 40%. This method can be widely used in various types of plasma diagnosis and provides a new idea for plasma diagnosis.