This study utilizes ArcGIS10.8 and ENVI5.3 software and the InVEST model to analyze and operate field survey data and remote sensing image data from Ruoergai National Park. The work simulates the soil retention, carbon storage, water supply, and habitat quality of the park to evaluate and analyze its four major ecosystem services. Subsequently, important areas of ecosystem services are zoned based on the results, aiming to provide decision-makers with a theoretical and scientific basis for formulating ecological environment restoration, protection, and management measures in Ruoergai National Park. The results showed the following: (1) In the study area, the land use types, ranked from largest to smallest, are grassland, unused land, forest, water area, and construction land. (2) Soil retention and water supply show an increasing trend, while carbon storage shows a decreasing trend. Habitat quality remains relatively stable, with most areas maintaining a high level of quality. (3) The importance zoning of ecosystem services in the study area exhibited a trend of “four increases and one decrease”. Specifically, the areas classified as moderately important, highly important, and extremely important all increased, while the area designated as generally important decreased. The findings indicate that climate change, land use type changes, and human activities are the primary factors influencing changes in ESs. It is crucial to prioritize highly important and extremely important areas for protection and utilization within Ruoergai National Park. Moving forward, it will be essential to minimize human activities that disrupt the ecosystem, while also focusing on the conservation and sustainable use of forest and grassland.