Research Study of the Conditions of Wastes Lipolysis Lipid Fraction

Sklyar, Victoria; Krussir, Galina; Lebedenko, T.; Хомич, Галина Панасівна; Коваленко, Ірина Вікторівна

doi:10.12911/22998993/99745

Cited by 4 publications

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“…In previous works, the efficiency of the use of hydrogenated fat was shown (Sklyar et al, 2019) hydrogenated fat with the release of waste generate provided. Figure 1 shows the scheme of processing waste where A -is the amount of 0.05 M NaOH, which was used for titration of the prototype, cm³; A 1 -quantity of 0.05 M NaOH, which was used for titration of the control sample, cm³; B -amount of enzyme in the reaction mixture, mg; 100 -coefficient for conversion to μM; LA -lipolytic activity, U.…”

Section: Results Andmentioning

confidence: 99%

“…In previous works, the efficiency of the use of Rhizopus japonicus lipase in the hydrolysis of hydrogenated fat was shown (Sklyar et al, 2019), a technological scheme for the production of hydrogenated fat with the release of waste generated during its production (Skliar et al, 2019) was provided. Figure 1 shows the scheme of processing waste with immobilized lipase.…”

Section: Resultsmentioning

confidence: 99%

“…The simplest and most basic way to solve the problem of disposal of spent bleaching clay is a technology that involves their removal to landfills of solid waste. The most resource-efficient and environmentally friendly method is recycling with the production of a number of technical products, the technology of which uses the biotechnological method (Sklyar et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…The results of the studies (Sklyar et al, 2019) showed the effectiveness of the use of the enzyme lipase Rhizopus japonicus for hydrolysis of hydrogenated fat, and the physical and chemical properties of native lipase were studied (Skliar et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Study of the physical and chemical characteristics of an immobilized lipase in the hydrolysis of fat waste

Skliar

Krusir

Iryne

et al. 2020

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A large amount of fatty waste from food enterprises accumulates in Ukraine, which represents a number of problems in their disposal. The main ones are formed during the production and processing of vegetable oils. Fat conversion usually occurs at high temperatures and pressures, while enzymatic hydrolysis is an energy-saving process. Work highlights the main factors for obtaining immobilized biocatalysts, the conditions and methods for determining the activity and stability of immobilized enzymes. The work is devoted to the study of the physical and chemical properties of the immobilized lipase Rhizopus japonicus, namely the influence of the pH of the medium (pH optimum pH stability) and temperature (thermal optimum, thermal stability). The objects of study were immobilized lipase Rhizopus japonicus, waste from the stage of demetalization of hydrogenated fat and activated carbon with a grain size of 2.0-2.8. It was revealed that for the immobilized lipase Rhizopus japonicus, the pH optimum value expanded with a shift from 7.0 to 6.5, and a significant increase in pH stability was observed during prolonged incubation of the immobilized preparation. It was established that lipase immobilization leads to an expansion of the thermos-optimum, as well as stabilization of the enzyme during prolonged incubation at a temperature of 40°C and at higher temperatures (60-80°C). The experimental results obtained indicate a higher stability of the immobilized lipase Rhizopus japonicus compared to native. The high activity and stability of immobilized lipase make it possible to recommend for bioconversion of oil and fat waste.

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Section: Results Andmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Study of the physical and chemical characteristics of an immobilized lipase in the hydrolysis of fat waste

Skliar

Krusir

Iryne

et al. 2020

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A Promising Way to Dispose of Fatty Waste by Hydrolysis and Study of the Conditions for Immobilization of Rhizopus Japonicus Lipase on Carriers

et al. 2020

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The hydrogenation process in oil and fat production is accompanied by the formation of a large amount of waste, the majority of which includes spent catalysts and spent sorbents. The bioconversion of lipids through the use of immobilized enzyme preparations expands the possibilities and is one of the most powerful resource potentials of environmental biotechnology. The adsorption methods are most effective, which is due to the ease of the binding process, the low cost of the carrier and the absence of toxic substances. Immobilization of adsorption provides a large surface area, which is important for the lipolytic enzymes performing catalysis at the interface. In most cases, adsorption slightly reduces the activity of lipases and, which is extremely important, does not affect their specificity. It was shown that the use of activated carbon with a grain size of 2.0-2.8 as a carrier for immobilization of lipase, leads to maximum preservation of the initial lipolytic activity. The weight ratio of carrier/ enzyme, optimal in terms of preservation of lipolytic activity, was 1 g of biopolymer carrier per 500 mg of lipase (1: 0.5) with preservation of 36.33% of the initial activity of the native enzyme. From the obtained experimental data, it follows that the rational conditions for the immobilization of Rhizopus japonicus is GM 1.5, temperature 25°С, duration of immobilization 15 minutes, the size of particles of activated carbon as a matrix is 2.0-2.8 mm. The lipolytic activity of the enzyme immobilized under these conditions is preserved by more than 30% compared with the native one, which is a high indicator of the preservation of activity.

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Investigation of the Fat Fraction Enzymatic Hydrolysis of the Waste From Production of Hydrogenated Fat by the Lipase Rhizopus Japonicus

Skliar¹,

Krusir²,

Zakharchuk³

et al. 2019

ХНТ

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. In the article the conditions of enzymatic hydrolysis of fat fraction of waste from production of hydrogenated fat by the lipase Rhizopus japonicus are considered, namely, the influence of pH of the medium (pH-optimum, pH-stability) and temperature (thermal optimum, thermal stability). The scope of applications of lipases in various branches of the national economy, including for utilization of numerous fatty waste and by-products of oil and fat industry, is disclosed. The main reasons of biotechnological potential of microbial lipases are considered. Objects of research were the lipase Rhizopus japonicus and waste from the demetallization stage of the hydrogenated fat production. Detected, that the optimum pH value for Rhizopus japonicus lipase is 7.0, reducing the pH of the medium from the optimum to pH 6.0 is accompanied by a decrease in activity by 30%, and an increase from 7.0 to 9.0 – decrease by 20%. The maximum activity of the enzyme is observed in the region of physiological values of the temperature. It has been established that the lipase optimal temperature is 40°C. The results of the Rhizopus japonicus lipase stability study showed that incubation of the enzyme at pH 2.5 resulted in a complete loss of lipolytic activity after 30 minutes, and at alkaline pH, the enzyme was more stable. Incubation of lipase Rhizopus japonicus for 30 min at pH 9.0 leads to loss of lipolytic activity by 25% of the maximum, and total loss of activity occurs after 2.5 h. The study of pH-stability of Rhizopus japonicus lipase at an optimal pH of 7 showed that after 60 min of incubation, the enzyme lost 15% of the lipolitical activity, and after 60 min – 50%. Complete loss of Rhizopus japonicus lipase activity at pH 7.0 takes place after 150 minutes of incubation. The results of the study of thermal stability of lipase showed that at a temperature of 40°C and 60°C, the lipase activity remained rather stable for 50 minutes and completely lost after 150 minutes of incubation. At 80°C and 100°C, lipase activity was lost after 40 minutes and 50 minutes of incubation, respectively. The results of the study indicate the prospect of enzymatic hydrolysis of fat fraction of waste by Rhizopus japonicus lipase. The results obtained should be used to improve the processing technology of waste oil and fat industry food and processing industries.

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Research Study of the Conditions of Wastes Lipolysis Lipid Fraction

Cited by 4 publications

References 1 publication

Study of the physical and chemical characteristics of an immobilized lipase in the hydrolysis of fat waste

Study of the physical and chemical characteristics of an immobilized lipase in the hydrolysis of fat waste

A Promising Way to Dispose of Fatty Waste by Hydrolysis and Study of the Conditions for Immobilization of Rhizopus Japonicus Lipase on Carriers

Investigation of the Fat Fraction Enzymatic Hydrolysis of the Waste From Production of Hydrogenated Fat by the Lipase Rhizopus Japonicus

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