Background: Per- and polyfluoroalkyl substances (PFAS) are a large group of manufactured chemicals. Since the beginning of their commercial manufacturing in the 1950s, PFAS haven’t only found their way into numerous industrial and commercial applications, but also into the bloodstream of the majority of the human population, the natural environment and its wildlife. Exposure to high levels of PFAS can create health risks for humans and animals which may exacerbate the effects of other anthropogenic impacts faced by wildlife species. To gain a comprehensive overview of the abundance and distribution of PFAS in wildlife species, and to better understand the risk of PFAS exposure on threatened species and PFAS transfer into human food chains, we will collate the available literature into a systematic evidence map and bibliometric analysis.Methods: We will conduct a comprehensive systematic literature search on Scopus, Web of Science and the ‘grey literature’. For screening purposes, we will use decision trees, scanning title, abstract and keywords first. The next step includes full-text screening performed by two reviewers. We will only consider publications in English, peer-reviewed articles, pre-prints and theses. We will limit our search to 31 PFAS types (based on a previous study). A pilot search on Scopus resulted in ~250 potentially relevant publications. We will scan all publications included in the systematic map for predetermined indicators of quality and potential study-level biases. In addition, we will extract bibliometric records from Scopus and perform network analysis. We will present the results using a narrative summary, tables (database), bar plots and colour-coded maps. Results will be available on a dedicated freely accessible website. Discussion: This study will provide critical insight into the gaps and clusters of the literature with regards to the PFAS concentration in wildlife. Therefore, our study will inform and direct future research efforts to fill the gaps revealed.Systematic review registration: osf.io osf.io/gnt2y