An experiment entitled, "Assessment of Capsicum (Capsicum annuum) Varietal Performance in Natural Ventilation Polyhouse Settings." was conducted at Horticulture Research Farm, Department of Horticulture, during the year 2022-23. Ten Capsicum Varieties, Names Sweet Pepper Nemalite, Sweet Pepper Cebrail, Sweet Pepper Bungi. Capsicum Varun, Sweet Pepper Volante, Shehzadi. Alpine 8510, Alpine 8248, Capsicum Orange and Capsicum IMP were evaluated at SHUATS, Prayagraj in randomized block design with three replications during winter season 2022-23 to evaluate best performing variety in terms of growth, yield and quality. The Sweet Pepper Volante was found maximum performance with the maximum fruit weight (154.20g), Fruit length (8.83cm), Fruit yield per plant (1.47 kg), Fruit yield (17.72 q/200 sq. m.). Sweet Pepper Volante was found superior based on overall performance in term of growth, yield and quality and highest cost benefit ratio (2.79) was found for capsicum in Sweet Pepper Volante variety under naturally ventilated polyhouse condition.