Reshaping the active pocket of promiscuous lactonases for degrading bulky organophosphate flame retardants

Abstract

Engineering of a promiscuous lactonase via semi-rational evolution gave a 1007-fold improvement in its catalytic activity in the degradation of triphenyl phosphate (TPHP). TPHP is a typical bulky organophosphate flame...

Organophosphate Esters (OPEs) Flame Retardants in Water: A Review of Photocatalysis, Adsorption, and Biological Degradation

“…Enzymatic hydrolysis was a green technique to neutralize these refractory organic matters. Unlike the naturally occurring enzymes sb-PTE that could degrade OPEs, Zhang et al [ 85 ] achieved 99% degradation of TPhP in 24 h and complete degradation in 48 h by introducing two mutant gene synthetic variants of PoOPHv5. The high hydrolysis activity of the biocatalyst was attributed to the synergistic effect of the mutation, which increased the hydrophobicity of the substrate binding bag and broadened the exit channel of the product.…”

Engineering Transcription Factor XylS for Sensing Phthalic Acid and Terephthalic Acid: An Application for Enzyme Evolution

“…Transcription factor (TF)-based whole-cell biosensors provide a flexible protocol that couples detection of target molecules with the downstream expression of fluorescent reporter proteins, enabling the fluorimetric detection of molecules and screening of protein variants. − Detection using whole-cell biosensors relies on the binding of target molecules to TFs. To date, many TFs have been identified as recognizing various small molecules. − TphR from Comamonas sp.…”