Residual Brewing Yeast as Substrate for Co-Production of Cell Biomass and Biofilm Using Candida maltosa SM4

Abstract

Candida maltosa was cultivated in the liquid phase of residual brewing yeast, a major brewery residue, to produce biomass and biofilm. Using response surface methodology, the effect of two variables at two different levels was investigated. The independent variables were agitation speed (at 100 and 200 rpm), and aeration (at 1 and 3 L min−1). Aeration was identified to be important for the production of both biomass and biofilm, while agitation was the only factor significantly affecting biofilm production. The maximal production of biofilm (2.33 g L−1) was achieved for agitation of 200 rpm and aeration of 1 L min−1, while the maximum for biomass (16.97 g L−1) was reached for 100 rpm agitation and 3 L min−1 air flow. A logistic model applied to predict the growth of C. maltosa in the exponential phase and the biofilm production, showed a high degree of agreement between the prediction and the actual biomass measured experimentally. The produced biofilms were further characterized using Fourier-transform infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and Thermogravimetric Analysis (TGA). FTIR allowed the identification of methyl, carbonyl ester and sulfate groups, and revealed the presence of uronic acid moieties and glycosidic bonds. Water-retention ability up to relatively high temperatures was revealed by TGA, and that makes the produced biofilm suitable for production of hydrogels. SEM also gave indications on the hydrogel-forming potential of the biofilm.

High-Temperature, Noncatalytic Oxidation of Polyethylene to a Fermentation Substrate Robustly Utilized by Candida maltosa

“…68 Other researchers also assert that C. maltosa can be used for SCP production. 68,69 Production of SCP from carbon thought not to be valuable (e.g., waste plastic) can increase the resiliency of global food production systems and divert waste from landfills. 21 The current findings provide the foundation for the extension of the microorganisms employed for SCP production and for waste plastic valorization.…”

Production of pyruvic acid with Candida glabrata using self-fermenting spent yeast cell dry powder as a seed nitrogen source

“…There are differences in dissolved oxygen levels and mechanical injury between shake flask fermentation and fermenter fermentation. Dissolved oxygen levels and shear forces from excessive agitation affect cell growth and product synthesis (Flores-Copa et al 2021 ; Snopek et al 2021 ).…”

Biosorption Potential of Microbial and Residual Biomass of Saccharomyces pastorianus Immobilized in Calcium Alginate Matrix for Pharmaceuticals Removal from Aqueous Solutions

“…Residual biomass of Saccharomyces pastorianus , which is considered a second-largest by-product of the brewing industry, contains significant residual carbohydrates, proteins, aminoacids, lipids, minerals, and enzymes, and it is still investigated for obtaining high added-value products [ 35 ]. If we consider the aspects mentioned above, it turns out that this residue seems to meet all the requirements for use in obtaining a viable biosorbent: it is safe, low-cost, and available throughout the year in large volumes [ 31 ].…”