Residue Analysis and Assessment of the Risk of Dietary Exposure to Domoic Acid in Shellfish from the Coastal Areas of China

Abstract

Harmful algal blooms in Chinese waters have caused serious domoic acid (DA) contamination in shellfish. Although shellfish are at particular risk of dietary exposure to DA, there have been no systematic DA risk assessments in Chinese coastal waters. A total of 451 shellfish samples were collected from March to November 2020. The presence of DA and four of its isomers were detected using liquid chromatography–tandem mass spectrometry. The spatial-temporal distribution of DA occurrence and its potential health risks were examined. DA was detected in 198 shellfish samples (43.90%), with a maximum level of 942.86 μg/kg. DA was recorded in all 14 shellfish species tested and Pacific oysters (Crassostrea gigas) showed the highest average DA concentration (82.36 μg/kg). The DA concentrations in shellfish showed distinct spatial-temporal variations, with significantly higher levels of occurrence in autumn than in summer and spring (p < 0.01), and particularly high occurrence in Guangdong and Fujian Provinces. The detection rates and maximum concentrations of the four DA isomers were low. While C. gigas from Guangdong Province in September showed the highest levels of DA contamination, the risk to human consumers was low. This study improves our understanding of the potential risk of shellfish exposure to DA-residues.

Online highly selective recognition of domoic acid by an aptamer@MOFs affinity monolithic column coupled with HPLC for shellfish safety monitoring

“…In most prior literature, the maximum concentrations of DA residue in the natural shellfish samples were 206.35 μg kg −1 in clams and 257.80 μg kg −1 in mussels, respectively. 35 Compared with the in-tube MIP monolith (100 mm length, 4.6 mm, i.d. ), the binding capacity in this method was less, due to the trace amount of ZIF-8 MOF and the aptamer grafted on the capillary monolith.…”

Explainable machine learning for predicting the geographical origin of Chinese Oysters via mineral elements analysis

“…In 2022, China's oyster production is projected to exceed 6.20 million tons, primarily concentrated in the provinces of Liaoning, Fujian, Shandong, Guangdong and Guangxi ( MOAC, 2023 ). In recent years, an outbreak of dangerous algal blooms, specifically harmful microalgae, has led to the detection of various toxins, such as dinophysistoxin-2 and domoic acid, in oysters sourced from Guangdong and Guangxi provinces ( Zheng et al, 2022 ). Moreover, oysters possess the ability to accumulate toxic elements such as cadmium in their tissues ( Ng et al, 2010 ).…”

Recombinase Polymerase Amplification Combined with Lateral Flow Dipstick Assay for the Rapid and Sensitive Detection of Pseudo-nitzschia multiseries

“…Shellfish are the dominating cultured organisms and a potential vector for algal toxins [8]. Given the alarming increase in HABs in China, increased attention should be paid to all the HABs that have the potential to cause DA contamination [16]. Hence, to provide a rapid emergency response to the HABs of Pseudo-nitzschia species, including P. multiseries, it is necessary to establish an accurate and rapid point-of-care detection approach [17].…”