Resistance evolution, from genetic mechanism to ecological context

Baucom, Regina S.; Iriart, Veronica; Kreiner, Julia M.; Yakimowski, Sarah B.

doi:10.1111/mec.16224

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…The Special Feature published this year "Resistance evolution, from genetic mechanism to ecological context" was edited by Regina Baucom, Ana Caicedo, Daniel Croll, Kenneth Olsen and Sarah Yakimowski. As pointed out in the introduction to the special issue (Baucom et al, 2021), this suite of papers builds on a long history of understanding adaptation through the lens of resistance to pesticides, herbicides, and fungicides. The first section of the special feature focuses on the genetic and genomic insights garnered by studies of resistance evolution.…”

Section: Special Issuesmentioning

confidence: 99%

Editorial 2022

et al. 2021

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Section: Special Issuesmentioning

confidence: 99%

Editorial 2022

et al. 2021

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“…The development of pesticide resistance is an evolutionary process involving selection by the pesticide, fitness effects of resistance mutations in the absence of the pesticide, genetic linkage, recombination of resistance alleles, and gene flow among populations. [41][42][43] When a population is under continuous selection by a pesticide, the frequency of resistant alleles will increase. The selection at one locus may increase the frequency of alleles at linked loci, including those that also confer pesticide resistance.…”

Section: Introductionmentioning

confidence: 99%

A high‐throughput KASP assay provides insights into the evolution of multiple resistant mutations in populations of the two‐spotted spider mite Tetranychus urticae across China

Shen

Zhang

Wang

et al. 2023

Pest Management Science

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Background: The two-spotted spider mite (TSSM), Tetranychus urticae (Acari: Tetranychidae), is a cosmopolitan phytophagous pest in agriculture and horticulture. It has developed resistance to many acaricides by target-site mutations. Understanding the status and evolution of resistant mutations in the field is essential for resistance management. Here, we applied a highthroughput Kompetitive allele-specific polymerase chain reaction (KASP) method for detecting six mutations conferring resistance to four acaricides of the TSSM. We genotyped 3274 female adults of TSSM from 43 populations collected across China in 2017, 2020, and 2021.Results: The KASP genotyping of 24 testing individuals showed 99% agreement with Sanger sequencing results. KASP assays showed that most populations had a high frequency of mutations conferring avermectin (G314D and G326E) and pyridaben (H92R) resistance. The frequency of mutation conferring bifenazate (A269V and G126S) and etoxazole (I1017F) resistance was relatively low. Multiple mutations were common in the TSSM, with 70.2% and 24.6% of individuals having 2-6 and 7-10 of 10 possible resistant alleles, respectively. No loci were linked in most populations among the six mutations, indicating the development of multiple resistance is mainly by independent selection. However, G314D and I1017F on the nuclear genome deviated from Hardy-Weinberg equilibrium in most populations, indicating significant selective pressure on TSSM populations by acaricides or fitness cost of the mutations in the absence of acaricide selection.Conclusion: Our study revealed that the high frequency of TSSMs evolved multiple resistant mutations in population and individual levels by independent selection across China, alarming for managing multiple-acaricides resistance.

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Repeated evolution of herbicide resistance inLolium multiflorumrevealed by haplotype-resolved analysis of acetyl-CoA carboxylase

Brunharo

Tranel

2023

Preprint

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Herbicide resistance in weeds is one of the greatest challenges in modern food production. The grass species Lolium multiflorum is an excellent model species to investigate convergent evolution under similar selection pressure because populations have repeatedly evolved resistance to many herbicides, utilizing a multitude of mechanisms to neutralize herbicide damage. In this work, we investigated the gene that encodes acetyl-CoA carboxylase (ACCase), the target-site of the most successful herbicide group available for grass weed control. We sampled L. multiflorum populations from agricultural fields with history of intense herbicide use, and studied their response to three ACCase-inhibiting herbicides under controlled conditions. To elucidate the mechanisms of herbicide resistance and the genetic relationship among sampled populations, we resolved the haplotypes of 97 resistant and susceptible individuals by performing an amplicon-seq analysis using long-read DNA sequencing technologies, focusing on the DNA sequence encoding the carboxyl-transferase domain of ACCase. Our dose-response data indicated the existence of many, often unpredictable, resistance patterns to ACCase-inhibiting herbicides, where populations exhibited as much as 37-fold reduction in herbicide response. The majority of the populations exhibited resistance to all three herbicides studied. Phylogenetic and molecular genetic analyses revealed multiple evolutionary origins of resistance-endowing ACCase haplotypes, as well as widespread admixture in the region regardless of cropping system. The amplicons generated were very diverse, with haplotypes exhibiting 26 to 110 polymorphisms. Polymorphisms included insertions and deletions 1-31 bp in length, none of which were associated with the resistance phenotype. We also found evidence that some populations have multiple mechanisms of resistance. Our results highlight the astounding genetic diversity in L. multiflorum populations, and the potential for convergent evolution of herbicide resistance across the landscape that challenges weed management and jeopardizes sustainable weed control practices. We provide an in-depth discussion of the evolutionary and practical implications of our results.

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Resistance evolution, from genetic mechanism to ecological context

Cited by 3 publications

References 19 publications

Editorial 2022

Editorial 2022

A high‐throughput KASP assay provides insights into the evolution of multiple resistant mutations in populations of the two‐spotted spider mite Tetranychus urticae across China

Repeated evolution of herbicide resistance inLolium multiflorumrevealed by haplotype-resolved analysis of acetyl-CoA carboxylase

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