Resistance levels and fitness of protoporphyrinogen oxidase (PROTOX) inhibitor-resistant transgenic rice in paddy fields

Jung, Ha-il; Kuk, Yong In; Kim, H.Y.; Back, Kyoungwhan; Lee, D.J.; Lee, S.; Burgos, Nilda R.

doi:10.1016/j.fcr.2009.10.010

Cited by 15 publications

(8 citation statements)

References 23 publications

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“…This holds true for our study system. Although we did not detect any performance cost of monocultured transgenic plants when compared to non-transgenic plants, a previous study reported reduced yield of a transgenic rice line transformed with a PPO gene that is different with ours (Jung et al 2010). Therefore, such a cost must be studied case-by-case for a given trait, species, and transgene.…”

Section: Genotypecontrasting

confidence: 99%

Fitness cost and competitive ability of transgenic herbicide-tolerant rice expressing a protoporphyrinogen oxidase gene

Chun¹,

Kim²,

Park³

et al. 2013

J. Ecol. Environ.

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The expression of transgenic traits in genetically modified crops is sometimes associated with decreases in crop performanceor fitness. These decreases in performance or fitness of transgenic plants in unfavourable conditions may providevaluable information about the ecological consequences of transgene escape. In a glasshouse trial, we tested the costassociated with resistance to herbicides by comparing the growth, yield, and competitive ability of transgenic rice withits parental non-transgenic line. This new line was developed for constitutive overexpression of protoporphyrinogen oxidase(PPO) to increase resistance to herbicides. We evaluated nine agronomic traits of transgenic and non-transgenic ricegrown in a replacement series design over four densities. Competitive ability was also assessed between transgenic andnon-transgenic plants by analyzing their relative yields based on biomass and seed weight data. Our results indicated thatnon-transgenic plants showed greater performance than did the transgenic plants when those genotypes were grownin mixtures. The non-transgenic rice plants exhibited superior competitive ability at certain combinations of plantingdensities and genotype proportions. These results suggest that PPO-herbicide resistance incurs some costs in plant performanceand competitive ability

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Section: Genotypecontrasting

confidence: 99%

Fitness cost and competitive ability of transgenic herbicide-tolerant rice expressing a protoporphyrinogen oxidase gene

Chun¹,

Kim²,

Park³

et al. 2013

J. Ecol. Environ.

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“…These results highlight the problem of gene flow from resistant rice cultivars, resulting in a limitation of this technology due to migration of the resistance gene to weedy red rice. However, several transgenic technologies are being developed for glufosinate ammonium resistance (Gealy et al 2003;Michiels and Johnson 2001), glyphosate resistance (Hu et al 2009;Zhou et al 2006), and protoporphyrinogen oxidase inhibitors (Jung et al 2010). In addition, new natural-mutant rice cultivars resistant to herbicides other than the ALS inhibitors are currently being developed (Merotto et al 2013).…”

Section: Resistancementioning

confidence: 99%

Distribution of Weedy Red Rice (Oryza sativa) Resistant to Imidazolinone Herbicides and its Relationship to Rice Cultivars and WildOryzaSpecies

Goulart¹,

Borba²,

Menezes³

et al. 2014

Weed sci.

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Several weedy red rice populations have evolved resistance to imidazolinone herbicides worldwide. The understanding of the factors related to the herbicide resistance in weedy red rice is important to prevent its occurrence in new areas where imidazolinone-resistant rice cultivars are being used, and to manage the new rice cultivars resistant to herbicides with modes of action other than the acetolactate synthase (ALS)-inhibitors that are being developed. The objectives of this study were to analyze the relationship of weedy red rice populations from southern Brazil with rice cultivars and wild Oryza species and to evaluate the occurrence of introgression from rice cultivars and seed migration as the origin of resistance to imidazolinone herbicides in weedy rice. The study was based on 27 weedy red rice populations, seven rice cultivars, and four wild Oryza species that were genotyped with 24 simple sequence repeats and three ALS-specific single-nucleotide polymorphism markers. A large proportion of the genetic variation of the weedy red rice populations was found within (74%) rather than among populations (26%). The weedy red rice populations were more closely related to the newer rice cultivars that are imidazolinone-resistant than to the older cultivars. The South American native Oryza glumaepatula and the other wild Oryza species—Oryza rufipogon, Oryza longistaminata, and Oryza glaberrima—clustered separately from weedy red rice populations, indicating a low likelihood of introgression among weedy red rice and these wild species. Seed migration was an important factor in the genetic structure of the evaluated weedy red rice populations, although gene flow by pollen from resistant cultivars was the principal reason for the spread of herbicide resistance.

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“…Derived from a soil bacterium, Myxococcus xanthus, this gene is known to be susceptible to the PPO inhibitor acifluorfen by itself (Dailey and Dailey 1996). Although the exact mechanism by which Mx PPO gene confers herbicide resistance is still unknown, transformation studies with this gene have resulted in rice with very high resistance to PPO inhibitors such as oxyfluorfen, acifluorfen, butafenacil, carfentrazone-ethyl, and oxadiazon (Jung and Back 2005;Jung and Kuk 2007;Lee et al 2007;Jung et al 2008Jung et al , 2010. The overproduction of PPOs is thought to prevent the accumulation of protoporphyrin in the cytosol and to mitigate the phytotoxic processes (Lermontova and Grimm 2000;Li and Nicholl 2005).…”

Section: Introductionmentioning

confidence: 98%

Two-year field study shows little evidence that PPO-transgenic rice affects the structure of soil microbial communities

et al. 2011

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There is global concern about the environmental consequences associated with transgenic crops. Their effects on the soil ecosystem are of special interest when assessing ecological safety and integrity. Although many efforts have been made to develop crops genetically modified to have resistance to protoporphyrin oxidase (PPO)-inhibiting herbicides, little is known about their influence on soil microbial communities. We conducted a 2-year field study and an analysis via terminal restriction fragment length polymorphism (T-RFLP) to assess the impacts of PPO-transgenic rice on bacterial and fungal communities. In the first year we sampled the rhizosphere and surrounding bulk soil, while in the second year we sampled rhizosphere soil only. No differences were observed in the diversity indices and community composition of microbial communities between transgenic rice and its parental non-transgenic counterpart (cultivar Dongjin). Instead, community variation was strongly dependent on growth stage and year. Therefore, we observed no adverse effects by these crops of modified rice on the microbial community composition in paddy soils.

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Resistance levels and fitness of protoporphyrinogen oxidase (PROTOX) inhibitor-resistant transgenic rice in paddy fields

Cited by 15 publications

References 23 publications

Fitness cost and competitive ability of transgenic herbicide-tolerant rice expressing a protoporphyrinogen oxidase gene

Fitness cost and competitive ability of transgenic herbicide-tolerant rice expressing a protoporphyrinogen oxidase gene

Distribution of Weedy Red Rice (Oryza sativa) Resistant to Imidazolinone Herbicides and its Relationship to Rice Cultivars and WildOryzaSpecies

Two-year field study shows little evidence that PPO-transgenic rice affects the structure of soil microbial communities

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