Resistance of Rapistrum rugosum to tribenuron and imazamox due to Trp574 or Pro197 substitution in the acetolactate synthase

Ntoanidou, Symela; Madesis, Panagiotis; Eleftherohorinos, Ilias G.

doi:10.1016/j.pestbp.2018.12.001

Cited by 9 publications

(8 citation statements)

References 32 publications

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“…However, the non-targetsite-based resistance (NTSR) could not be absolutely excluded out and might be possibly involved in conferring herbicide resistance. One resistant weed population may simultaneously contain target-site-based resistance (TSR) and NTSR (Ntoanidou et al, 2019). Moreover, NTSR usually conferred lower resistance on weed than TSR (Li et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Target-site basis for resistance to flucarbazone- sodium in Japanese brome (Bromus japonicus Houtt.) in China

Guo³

et al. 2022

Chil. j. agric. res.

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Japanese brome (Bromus japonicus Houtt.) is a troublesome annual weed and widely distributed in winter wheat (Triticum aestivum L.) fields in the North China Plain. A B. japonicus population (TJ06) suspected of resistance to acetolactate synthase (ALS) inhibitors was found in Tianjin, China. In this study, the TJ06 population with an Asp-376-Glu mutation in ALS gene was identified. TJ06 population developed 66.7-fold resistance to flucarbazone-sodium and exhibited obvious cross-resistance to other two ALS-inhibiting herbicides. The 50% plant growth reduction (GR50) to herbicides of pyroxsulam and mesosulfuron-methyl were 28.72 and 39.44 g ai ha -1 , respectively. In in vitro ALS activity assays, the concentration of flucarbazone-sodium required to inhibit 50% ALS activity (I50) for TJ06 was 11.3-fold greater than that for a susceptible population (TJ01), which was highly correlated with that of whole-plant response experiments and indicated that the Asp-376-Glu mutation leading to resistance reduced sensitivity of the ALS enzyme to flucarbazonesodium. Besides, one derived cleaved amplified polymorphic sequence (dCAPS) marker was designed to quickly detect Asp376 mutation in ALS gene of B. japonicus.

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Section: Discussionmentioning

confidence: 99%

Target-site basis for resistance to flucarbazone- sodium in Japanese brome (Bromus japonicus Houtt.) in China

Guo³

et al. 2022

Chil. j. agric. res.

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“…Trp-574-Leu has the ability to develop resistance to all groups of ALS inhibitor herbicides ( Pandolfo et al, 2016). The Trp-574 Leu mutation occurs with a higher level of resistance against IMI (Imazamox) and SU (Tribenuron methyl), according to various studies (Tranel and Wright, 2002;Warwick, Sauder & Beckie, 2005;Tan & Medd, 2002;Rosaria et al, 2011;Christoffers et al, 2006;Deng et al, 2017;Ntoanidou et al, 2017;Ntoanidou et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Studies in Turkey to assess the resistance to ALS inhibiting herbicides were conducted at a bioassay level ( Avcı, 2009 ; Aksoy et al, 2010 ; Uygur et al 2014 ; Gürbüz, 2016 ). One study to determine the molecular detection of chlorsulfuron resistance of wild mustard was conducted but did not find the point mutation ( Topuz, 2007 ).…”

Section: Discussionmentioning

confidence: 99%

Trp-574-Leu mutation in wild mustard (Sinapis arvensis L.) as a result of als inhibiting herbicide applications

Şin

Kadıoğlu

2021

PeerJ

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Wheat is one of the most important crops grown all around the world. Weeds cause significant yield loss and damage to wheat and their control is generally based on herbicide application. Regular use leads to herbicide resistance in weeds. This study aims to reveal molecular detection of Sinapis arvensis resistance mutation against ALS inhibiting herbicides. For this purpose, survey studies have been carried out in wheat growing areas in Amasya, Çorum, Tokat, and Yozgat provinces and wild mustard seeds have been collected from 310 different fields. According to bioassay tests with tribenuron-methyl, 13 of these populations, have not been affected by the registered dose of herbicide. When survived populations were subjected to dose-effect study and herbicides were applied at 26-fold, the highest and lowest resistance coefficients were determined as 7.2 (A-007) and 1.02 (T-034) respectively. In addition, B domain region from ALS gene was amplified and analyzed in molecular studies to determine point mutation in wild mustard against ALS herbicides. The PCR products were sequenced and target-site mutation to Leucine was observed at Trp-574 amino acide. In the study, point mutation in Trp-574 amino acide and Trp-574 Leu mutation in Sinapis arvensis have been detected for the first time in Turkey.

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“…Since this assay isolates the enzyme response in the presence of the herbicides, it can be used to predict potential target-site mutations [40,41]. For example, in tribenuron and imazamox resistant Rapistrum rugosum population, the RF at the enzyme level was greater than 270-fold, while the plant response in the greenhouse resulted in RF ranging from 40 to 370-fold [42]. In the same study, target site mutation at Trp574 and Pro197 imparting resistance were reported.…”

Section: Als Enzyme Assaymentioning

confidence: 93%

A Trp574Leu Target-Site Mutation Confers Imazamox Resistance in Multiple Herbicide-Resistant Wild Poinsettia Populations from Brazil

et al. 2020

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Wild poinsettia (Euphorbia heterophylla L.) is an important weed species in southern Brazil, especially due to the evolution of multiple herbicide resistance (e.g., acetolactate synthase (ALS)- inhibitors, protoporphyrinogen oxidase inhibitors, and glyphosate). The mechanism of resistance to imazamox was investigated in two wild poinsettia populations (R1 and R2) from southern Brazil and compared to a known susceptible (S) population. Imazamox dose-response experiments revealed high levels of resistance: 45-fold and 224.5-fold based on dry biomass reduction, for R1 and R2, respectively. Extremely high concentrations of imazamox (20,000 µM) were not sufficient to provide 50% inhibition of ALS enzyme activity (I50) for R1 or R2. Hence, resistance levels were estimated to be greater than 123-fold for both populations based on in vitro ALS assays. The ALS gene from all R1 and R2 plants had a Trp574Leu mutation. A genotyping assay was developed to discriminate resistant and susceptible alleles based on the Trp574Leu mutation.

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Resistance of Rapistrum rugosum to tribenuron and imazamox due to Trp574 or Pro197 substitution in the acetolactate synthase

Cited by 9 publications

References 32 publications

Target-site basis for resistance to flucarbazone- sodium in Japanese brome (Bromus japonicus Houtt.) in China

Target-site basis for resistance to flucarbazone- sodium in Japanese brome (Bromus japonicus Houtt.) in China

Trp-574-Leu mutation in wild mustard (Sinapis arvensis L.) as a result of als inhibiting herbicide applications

A Trp574Leu Target-Site Mutation Confers Imazamox Resistance in Multiple Herbicide-Resistant Wild Poinsettia Populations from Brazil

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