Resistance of Tangmai 4 wheat to powdery mildew, stem rust, leaf rust, and stripe rust and its chromosome composition

Abstract

The hard red winter wheat Tangmai 4 did not develop symptoms of infection following inoculation with powdery mildew (Erysiphe graminis DC. f. sp. tritici E. Marchal) isolates from regions of western Canada and northern China. Tangmai 4 exhibited resistance to stem rust (Puccinia graminis Pers. f. sp. tritici Eriks. & Henn.) and leaf rust (P. triticina Eriks.) races from western Canada. This wheat line was resistant to individual stripe rust (P. striiformis Westend. f. sp. tritici Eriks.) races from the U.S. and Canada. Sequential C-banding and genomic in situ hybridization (GISH), and electrophoretic analyses of high molecular weight glutenins and gliadins demonstrated that Tangmai 4 carried a pair of T1BL·1RS wheat-rye (Secale cereale L.) translocated chromosomes. Since the genes located on T1BL·1RS are no longer effective in controlling powdery mildew and the rust diseases, Tangmai 4 must carry additional genes for resistance to these diseases, which makes it a valuable resource for the improvement of resistance in wheat against these diseases. Key words: T1BL·1RS translocation, disease resistance, sequential C-banding and GISH, glutenin, gliadin

Fine mapping of powdery mildew resistance gene PmTm4 in wheat using comparative genomics

“…A powdery mildew resistant common wheat line Tangmai 4 (Li et al 2004;Hu et al 2008) was crossed with a highly susceptible common wheat line Xuezao (Zhang et al 2010) to produce F 1 hybrids, F 2 segregating populations and their F 2:3 families for fine mapping of powdery mildew resistance gene PmTm4 in Tangmai 4. Fuzhuang 30, Xiaobaidongmai, Hongquanmang, Mazhamai, and Laozaomai were used as donors of powdery mildew resistance genes Pm5e, xbd, PmH, Pmmz, and PmTm4.…”