Resistance Status and Associated Resistance Mechanisms to Certain Insecticides in Rice Weevil Sitophilus oryzae (Coleoptera: Curculionidae)

Abstract

The rice weevil, Sitophilus oryzae (Coleoptera: Curculionidae) was collected from El-Behera governorate (EB). The first generation showed reduced susceptibility to deltamethrin and malathion compared with the laboratory susceptible strain (LS). Susceptibility test results (using LS colony as a reference strain) indicated that EB strain has relative toxicity ratio, RR 50 and RR 90 of 16.64 and 9.08 for malathion. Although, EB strain was tolerant to deltamethrin with RR 50 of 5.2, it was marginally resistant to cypermethrin and entirely susceptible to permethrin. The biochemical results showed elevation in the activity of total esterase (ES), carboxylesterase (CE) and glutathione S-transferase (GST) which suggested that the metabolic resistance may has a key function in this population. Furthermore, acetylycholinesterase (AChE) activity in EB strain was 3.52 times higher compared with LS strain which pointed the insensitivity of AChE in EB strain. Moreover, esterase analysis indicated genetic polymorphism between EB and LS populations which might be attributed to selection pressure due to continuous exposure to insecticides. This study suggested the existence of malathion and deltamethrin resistance in EB strain which might be a consequence of biochemical alterations. The results of current study indicate the importance of continuous monitoring of resistance of stored product insect pests to plan successful management strategies.

Sublethal Effects of Chlorantraniliprole on the Mobility Patterns of Sitophilus spp.: Implications for Pest Management

“…Species of Sitophilus pose a major threat to the food industry worldwide because of their expansion capabilities through global commerce and the immense post-harvest crop losses they cause [ 55 , 56 ]. A rising global problem lies in insecticide-resistant strains of Sitophilus spp., on both organophosphates and pyrethroids, in regions of Egypt, Brazil, Australia, and South Korea [ 8 , 57 , 58 , 59 ]. Furthermore, their life cycles as primary pests require larvae to develop inside the kernel, rendering their control efforts difficult depending on the substrate [ 60 ].…”

Kerentanan Relatif Jenis Beras Terhadap Sitophilus oryzae L. (Coleoptera: Curculionidae) Pada Keadaan Kadar Air Rendah

“…Hama ini tersebar luas di daerah subtropis dan tropis (Hong et al, 2018). S. oryzae yang menyebabkan kerusakan pada komoditas serealia seperti beras, sorgum, gandum, jagung, dan kacang mete di penyimpanan (Attia et al, 2017;Mehta & Kumar, 2020). Kerusakan beras meliputi penyusutan berat dan perubahan kimiawi serta kontaminasi oleh racun kimiawi (mikotoksin)…”