Resistance Tests and Genetic Resources for Breeding Fusarium Root Rot Resistant Lettuce

Tsuchiya, Nobuaki; Fujinaga, Masashi; Ogiso, Hideki; Usui, T.; Tsukada, M.

doi:10.2503/jjshs.73.105

Cited by 15 publications

(7 citation statements)

References 3 publications

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“…Lettuce cultivars resistant to race 1, race 2, or both races have been discovered and have been used for breeding new cultivars resistant to these races (Tsuchiya et al 2004). Recently, a new cultivar of crisp-head lettuce resistant to race 1 was bred by Tsuchiya (2003).…”

Section: Introductionmentioning

confidence: 99%

Development of sequence tagged site markers to identify races of Fusarium oxysporum f. sp. lactucae

Shimazu

Yamauchi

Hibi³

et al. 2005

J Gen Plant Pathol

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By random amplified polymorphic DNA (RAPD) analysis of the representative isolates of each race of Fusarium oxysporum f. sp. lactucae, RAPD fragments of 0.6, 1.6, and 2.9 kb were obtained. The 0.6-kb RAPD fragment was common to the representative isolates of all three races. Amplification of the 1.6-and 2.9-kb fragments were unique to the isolates of races 1 and 2, respectively. Sequence tagged site (STS) marker FLA0001, FLA0101, and FLA0201 were generated from the 0.6-, 1.6-, and 2.9-kb RAPD fragments, respectively. Polymerase chain reaction (PCR) analysis showed that FLA0001 was common to all 49 isolates of F. oxysporum f. sp. lactucae. FLA0101 was specifically generated from all 23 isolates of race 1 but not from races 2 or 3. FLA0201 was specifically amplified from all 12 isolates of race 2 but not from races 1 or 3. In two isolates of F. oxysporum f. sp. lactucum, PCR amplified FLA0001 and FLA0101 but not FLA0201. On the other hand, these STS markers were not detected from isolates of five other formae speciales. Because these STS markers were not generated from isolates of other plant pathogenic fungi, bacteria, or plant materials examined in this study, PCR analysis combined with the three STS markers should be a useful means for rapid identification of races of F. oxysporum f. sp. lactucae.

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Section: Introductionmentioning

confidence: 99%

Development of sequence tagged site markers to identify races of Fusarium oxysporum f. sp. lactucae

Shimazu

Yamauchi

Hibi³

et al. 2005

J Gen Plant Pathol

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“…Inoculum density in the resistance test was 1/20. The test and its evaluation were the same as the methods by Tsuchiya et al (2004a) except for inoculum density.…”

Section: Exp 1 Breeding Of 'Chouya No 37'mentioning

confidence: 99%

“…The two lines were crossed in 1998. A resistance test was conducted in the same way as described by Tsuchiya et al (2004a). FOL F9501 (race 2) (Fujinaga et al, 2001) was cultured in a mixture of bran and vermiculite in 1 : 1.5 (v/v) until the spore density increased to 10 8 cell·ml −1 .…”

Section: Exp 1 Breeding Of 'Chouya No 37'mentioning

confidence: 99%

“…Crisp lettuce 'Kikugawa No. 102' was selected as a genetic resource with resistance to FOL race 2 (Tsuchiya et al, 2004a); however, this cultivar was very early bolting (Tsuchiya et al, 1997) and did not have good shipping traits in summer cultivation. Therefore it was necessary to improve these traits by pedigree breeding after artificial crossing through this breeding program.…”

Section: Introductionmentioning

confidence: 99%

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‘Chouya No. 37’, a Fusarium Root Rot (Race 2)-Resistant Lettuce

Tsuchiya

2009

J. Japan. Soc. Hort. Sci.

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Fusarium root rot of lettuce (RRL) caused by Fusarium oxysporum f. sp. lactucae (FOL) race 2 occurrs only in Nagano Prefecture, Japan. The area affected by the disease reached 17 ha in 2003. 'Chouya No. 37' crispheadtype lettuce (crisp lettuce), which is adaptable to summer cultivation, was bred with resistance to FOL race 2. As a maternal line to breed a resistant cultivar, the selection line of 'Kikugawa No. 102' was used as a genetic resource with resistance to FOL race 2. The resistant line was crossed with the selection line of crisp lettuce with slow bolting. The progeny had been selected by various methods until F 6 , 'Chouya No. 37' was bred. 'Chouya No. 37' is as highly resistant to FOL race 2 as its maternal line. Its resistance is superior to that of other lettuce cultivars that are adaptable to mid-summer cultivation in Nagano. 'Chouya No. 37' is very vigorous, its leaf color is green to slightly dark green, and its leaf luster is relatively high. The leaf margins have relatively shallow incisions, indentations, and mild undulations. The quality of the head upon harvest is categorized as "relatively good" to "good". It also has slow bolting in mid-summer.

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“…．一方，本病菌には病原性分化が認められ(土屋ら 2000) ，日本国内においてはこれまでにレース 1， 2 および 3 の発生が報告されている (Fujinaga et al 2001, Yamauchi et al 2004 (Yamauchi et al 2001, Tsuchiya et al 2004b, Garibaldi et al 2004) ．また，レース 1 抵抗性品種として「シナノホープ」 (Tsuchiya et al 2004a)が，レース 2 抵抗性品種としては「長・野 37 号」 (Tsuchiya 2009)が育成されている…”

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