Resistance to Potato virus Y in a Multitrait Potato Breeding Scheme without Direct Selection in Each Generation

Solomon-Blackburn, Ruth M.; Bradshaw, J. E.

doi:10.1007/s11540-007-9031-8

Cited by 15 publications

(16 citation statements)

References 15 publications

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“…Host-plant resistance in cotton cultivars is the best long term strategy to protect the plant against CLCuD and highly explored by breeders [22,23]. Several cultivated cotton varieties in Pakistan with resistance to CLCuD are derived from CP-15/2 and LRA-5166 and possess a very narrow genetic base [24] with possible detrimental consequences to withstand ever evolving CLCuV invading cotton crop.…”

Section: Discussionmentioning

confidence: 99%

Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences

et al. 2011

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Several important biological processes are performed by distinct functional domains found on replication-associated protein (Rep) encoded by AC1 of geminiviruses. Two truncated forms of replicase (tAC1) gene, capable of expressing only the N-terminal 669 bp (5'AC1) and C-terminal 783 bp (3'AC1) nucleotides cloned under transcriptional control of the CaMV35S were introduced into cotton (Gossypium hirsutum L.) using LBA4404 strain of Agrobacterium tumefaciens to make use of an interference strategy for impairing cotton leaf curl virus (CLCuV) infection in transgenic cotton. Compared with nontransformed control, we observed that transgenic cotton plants overexpressing either N-terminal (5'AC1) or C-terminal (3'AC1) sequences confer resistance to CLCuV by inhibiting replication of viral genomic and β satellite DNA components. Molecular analysis by Northern blot hybridization revealed high transgene expression in early and late growth stages associated with inhibition of CLCuV replication. Of the eight T(1) transgenic lines tested, six had delayed and minor symptoms as compared to nontransformed control lines which developed disease symptoms after 2-3 weeks of whitefly-mediated viral delivery. Virus biological assay and growth of T(2) plants proved that transgenic cotton plants overexpressing 5'- and 3'AC1 displayed high resistance level up to 72, 81%, respectively, as compared to non-transformed control plants following inoculation with viruliferous whiteflies giving significantly high cotton seed yield. Progeny analysis of these plants by polymerase chain reaction (PCR), Southern blotting and virus biological assay showed stable transgene, integration, inheritance and cotton leaf curl disease (CLCuD) resistance in two of the eight transgenic lines having single or two transgene insertions. Transgenic cotton expressing partial AC1 gene of CLCuV can be used as virus resistance source in cotton breeding programs aiming to improve virus resistance in cotton crop.

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Section: Discussionmentioning

confidence: 99%

Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences

et al. 2011

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“…Three cultivars have been reported to exhibit hypersensitive resistance although they tested as susceptible in this study. These cultivars were Desiree [15,39,41], Nicola [22] and Sebago [28]. Desiree has been reported to contain the Ny tbr hypersensitivity gene, which is active against PVY O [39,41], and to display hypersensitive reactions of severe necrosis, leaf drop, and stunting [39].…”

Section: Discussionmentioning

confidence: 99%

“…These cultivars were Desiree [15,39,41], Nicola [22] and Sebago [28]. Desiree has been reported to contain the Ny tbr hypersensitivity gene, which is active against PVY O [39,41], and to display hypersensitive reactions of severe necrosis, leaf drop, and stunting [39]. However, Desiree was also described as displaying non-hypersensitive symptoms of severe mosaic and leaf deformation [39], and Desiree has been noted as susceptible in another study that used ELISA to detect the virus [38].…”

Section: Discussionmentioning

confidence: 99%

Screening for Resistance to PVY in Australian Potato Germplasm

Slater

Schultz

Lombardi

et al. 2020

Genes

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Potatoes are an important human food crop, but have a number of yield limiting factors, including disease susceptibility. Potato virus Y (PVY) is found worldwide, and is one of the main virus problems for potato growers. PVY is transmitted by aphids and mechanically by machinery, tools and people, and symptoms are variable across cultivars and strains, including being symptomless in some cultivars. Therefore, breeding resistant cultivars is the best way to control this virus. This study phenotypically screened 74 of the main commercial cultivars and a few other select cultivars grown in Australia, in order to identify sources of resistance to PVY. The cultivars were screened against PVY O and PVY NTN , with 23 out of 71 resistant to PVY O and 13 out of 74 resistant to PVY NTN , and all these 13 were resistant to both strains. When the phenotypic screening was compared to the results listed on the European Cultivated Potato Database, the majority of results were found to be consistent. We then evaluated three molecular markers RYSC3, M45, and STM0003 for the extreme resistance genes Ry adg and Ry sto , to validate the usefulness of the markers for marker-assisted selection (MAS) on Australian germplasm. The degree of correlation between the resistance phenotypes and the RYSC3, M45, and STM0003 markers for Ry adg and Ry sto conferred PVY resistance was determined. Three cultivars amplified the RYSC3 marker, while the M45 marker amplified the same 3 and an additional 9. Of the 12 cultivars, 11 phenotyped as resistant, but 1 was susceptible. The STM0003 marker was amplified from only 2 cultivars that both had resistant phenotypes. The RYSC3, M45, and STM0003 markers were therefore able to identify all the 13 cultivars that were resistant to both strains of PVY. Therefore, these markers will enable the identification of genotypes with resistance to PVY, and enable PVY resistant parents to be used for the development of superior progeny; these genetic markers can be used for MAS in the Australian potato breeding program.Genes 2020, 11, 429 2 of 15 mosaic symptoms on the leaves, and this can create a major risk for the spread of the virus in certified seed schemes [8]. Furthermore, the lack of symptoms in infected plants means that visual inspection will generally be ineffective, and that such plants provide a source of PVY inoculum in production areas. Therefore, breeding resistant cultivars is the best way to control this virus and remove inoculum.An effective potato breeding program aims to breed new cultivars that have traits that are superior or equivalent to those of current cultivars. In order to do this, potential parents need to be identified, which contain genes that control at least 40 desirable traits [9,10]. A list of traits that are important for the breeding of improved cultivars in Australia and their genetic control were listed by Slater et al. [10]. A number of these characters are considered to be controlled by single dominant genes, as they exhibit qualitative phenotypic variation. Simko et al.[11] l...

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“…They comprised ten parents with field resistance to late blight assumed to be derived primarily from S. demissum; 12 with resistance to the white potato cyst nematode of which six had resistance derived from S. vernei, four from S. tuberosum Gp Andigena, and two from both these sources; and 14 with virus resistance derived from a number of sources including S. acaule, S. chacoense, S. demissum, S. microdontum and S. stoloniferum (Bradshaw and Ramsay 2005). After three cycles of selection, the pedigrees of the 15 selected clones could be traced back to 15 out of the 36 initial parents, four from the blight resisters, five from the PCN resisters and six from the virus resisters (Solomon-Blackburn and Bradshaw 2007).…”

Section: Methodsmentioning

confidence: 99%

“…The parents with resistance to pathotype Pa2/3 of G. pallida also had either major gene (H1) or quantitative resistance to pathotype Ro1 of G. rostochiensis, the golden potato cyst nematode, pathotypes Pa2/3 and Ro1 being the ones present in Britain. Parents were also included with resistance to potato leafroll virus, potato virus Y and potato virus X, but time and resources did not permit direct selection for virus resistance in each generation (Solomon-Blackburn and Bradshaw 2007). Such an overall combination of traits was, and still is, lacking in European potato cultivars, despite 50 years of breeding effort.…”

Section: Introductionmentioning

confidence: 99%

Improving the yield, processing quality and disease and pest resistance of potatoes by genotypic recurrent selection

Bradshaw¹,

Dale²,

Mackay³

2009

Euphytica

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A potato (Solanum tuberosum Gp Tuberosum) breeding programme is described and analysed in which resistances to late blight [Phytophthora infestans (Mont.) de Bary] and the white potato cyst nematode (Globodera pallida) have been combined with a modest increase in yield and acceptable fry colour for processing. It began in 1991 and has involved cycles of crossing, selection between from 120 to 145 progenies (full-sib families), and clonal selection within the selected progenies. We have shown that the breeding scheme can operate on a 3-year cycle with limited within progeny selection, and on a 5-or 6-year cycle with more extensive within progeny selection. Six years are required when resistance to late blight is assessed in the tubers as well as the foliage. The more extensive within progeny selection is recommended once genes have been combined from sufficient parents to achieve one's objectives. The yield increase after three cycles of indirect selection through breeders' visual preference was only modest because it was operating against a decrease which would occur in the absence of selection. A practical improvement in the scheme would be to increase the number of progenies assessed to over 200, given the moderate to high heritabilities of the progeny and clonal tests. But this would require a considerable effort because the success rate achieved with the potato pollinations was typical at just over 30%. In the fourth cycle we showed how new breeding objectives and germplasm could be accommodated whilst continuing to maintain progress, something that is important in any long term breeding strategy.

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Resistance to Potato virus Y in a Multitrait Potato Breeding Scheme without Direct Selection in Each Generation

Cited by 15 publications

References 15 publications

Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences

Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences

Screening for Resistance to PVY in Australian Potato Germplasm

Improving the yield, processing quality and disease and pest resistance of potatoes by genotypic recurrent selection

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