Resistance to Rhizoctonia Solani in Transgenic Tobacco

Dunsmuir, Pamela; Howie, William J.; Newbigin, Ed; Joe, L.; Penzes, Eva; Suslow, Trevor

doi:10.1007/978-94-017-0651-3_63

Cited by 3 publications

(2 citation statements)

References 5 publications

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“…Brogue et al (1991) showed an increased ability of tobacco plants to survive in Rhizoctonia solani infected soil and delayed development of disease symptoms in tobacco seedlings by expressing chitinase. Dunsmuir et al (1993) also confirmed the bacterial chitinase gene expressed at high levels. Besides the immunity against a fungal pathogen, overexpression of chitinase was also found to be effective to raise resistance of plants against bacterial pathogens, salinity stress and heavy metal stress (Dana et al 2006).…”

Section: Discussionsupporting

confidence: 60%

In vitro screening of guava plantlets transformed with endochitinase gene against Fusarium oxysporum f.sp. psidii

Mishra¹,

Jalil²,

Mishra³

et al. 2016

CZECH J GENET PLANT

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Mishra M., Jalil S.U., Mishra R.K., Kumari S., Pandey B.K. (2016): In vitro screening of guava plantlets transformed with endochitinase gene against Fusarium oxysporum f.sp. psidii. Czech J. Genet. Plant Breed., 52: 6-13.Guava wilt disease is a severe threat to guava growers all over the world. It is caused by the soil-borne fungus Fusarium oxysporum f.sp. psidii. To control the disease, the Trichoderma-endochitinase gene was first introgressed into guava (Psidium guajava L.). The transgenic plantlets were screened in vitro for resistance against the wilt pathogen. Six-months-old genetically transformed plants raised in cocopeat under in vitro conditions were inoculated with a 7-days old culture of F. oxysporum f.sp. psidii. The presence of the pathogen in the cocopeat medium was confirmed by cultural as well as PCR analysis using species-specific primers. The roots of transgenic plants were wounded to facilitate the entry of the pathogen. The histopathological analysis revealed the presence of mycelium in vascular bundles. However, none of the plants showed symptoms of wilt disease during the investigation. In vitro pathogen inhibition assay and subsequently spore germination assay revealed that the crude leaf extract of transformed plants inhibited the germination of fungal conidia. The leaf tissue studied for expression of endochitinase revealed that two transgenic plants showed very high activity of N-acetyld-glucosamine (0.741 and 0.738 µM/min/µg of protein, respectively) which clearly indicated that transgenic plants could not develop any symptoms of wilt disease due to overexpression of endochitinase.

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Section: Discussionsupporting

confidence: 60%

In vitro screening of guava plantlets transformed with endochitinase gene against Fusarium oxysporum f.sp. psidii

Mishra¹,

Jalil²,

Mishra³

et al. 2016

CZECH J GENET PLANT

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“…The expression of the chitinase gene was significantly stimulated by fungi (Xu et al 2008 ). Transgenic plants over-expressing chitinase gene showed enhanced resistance to fungal (Brogue et al 1991 ; Dunsmuir et al 1993 ; Oldach et al 2001 ) and bacterial pathogens (Oldach et al 2001 ). It is likely that the increased level of Chia2a is responsible, at least partially, for the enhanced resistance in spl5 mutant.…”

Section: Resultsmentioning

confidence: 99%

Proteomic analysis of a disease-resistance-enhanced lesion mimic mutant spotted leaf 5 in rice

Chen

Zhang

et al. 2013

Rice

172

202

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BackgroundA lesion-mimic mutant in rice (Oryza sativa L.), spotted leaf 5 (spl5), displays a disease-resistance-enhanced phenotype, indicating that SPL5 negatively regulates cell death and resistance responses. To understand the molecular mechanisms of SPL5 mutation-induced cell death and resistance responses, a proteomics-based approach was used to identify differentially accumulated proteins between the spl5 mutant and wild type (WT).ResultsProteomic data from two-dimensional gel electrophoresis showed that 14 candidate proteins were significantly up- or down-regulated in the spl5 mutant compared with WT. These proteins are involved in diverse biological processes including pre-mRNA splicing, amino acid metabolism, photosynthesis, glycolysis, reactive oxygen species (ROS) metabolism, and defense responses. Two candidate proteins with a significant up-regulation in spl5 – APX7, a key ROS metabolism enzyme and Chia2a, a pathogenesis-related protein – were further analyzed by qPCR and enzyme activity assays. Consistent with the proteomic results, both transcript levels and enzyme activities of APX7 and Chia2a were significantly induced during the course of lesion formation in spl5 leaves.ConclusionsMany functional proteins involving various metabolisms were likely to be responsible for the lesion formation of spl5 mutant. Generally, in spl5, the up-regulated proteins involve in defense response or PCD, and the down-regulated ones involve in amino acid metabolism and photosynthesis. These results may help to gain new insight into the molecular mechanism underlying spl5-induced cell death and disease resistance in plants.Electronic supplementary materialThe online version of this article (doi:10.1186/1939-8433-6-1) contains supplementary material, which is available to authorized users.

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Enhancement of the antifungal activity ofBacillus subtilisF29-3 by the chitinase encoded byBacillus circulans chiAgene

Chen

Wang²,

Huang³

2004

Can. J. Microbiol.

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Bacillus subtilis F29-3 is an antagonistic bacterium against a wide range of fungal species. In order to determine the effect of chitinase on the antifungal activity of B. subtilis F29-3, a 2.4-kb DNA fragment containing the chiA gene of Bacillus circulans WL-12 was ligated into a shuttle vector pHY300PLK and transformed into B. subtilis F29-3. A bioassay conducted on the culture supernatant showed that, in comparison to the B. subtilis control strain, B. subtilis F29-3 expressing the chiA gene exhibited a greater inhibition of spore germination of Botrytis elliptica, indicating that chitinase could enhance the antifungal function conferred by B. subtilis F29-3.

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Resistance to Rhizoctonia Solani in Transgenic Tobacco

Cited by 3 publications

References 5 publications

In vitro screening of guava plantlets transformed with endochitinase gene against Fusarium oxysporum f.sp. psidii

In vitro screening of guava plantlets transformed with endochitinase gene against Fusarium oxysporum f.sp. psidii

Proteomic analysis of a disease-resistance-enhanced lesion mimic mutant spotted leaf 5 in rice

Enhancement of the antifungal activity ofBacillus subtilisF29-3 by the chitinase encoded byBacillus circulans chiAgene

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