Resistance to tetracycline in <i>Escherichia coli</i> isolates from poultry meat: epidemiology, policy and perspective

Ljubojević, Dragana; Pelić́, Miloš; Puvača, Nikola; Milanov, Dubravka

doi:10.1017/s0043933917000216

Cited by 27 publications

(21 citation statements)

References 44 publications

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“…On the contrary, Z i b a n d e h et al [24] reported the tet(A) to be the only tetracycline resistance gene detected in 72.5 % of E. coli isolates taken from the chickens on the day before slaughter.Tetracyclines are broad spectrum antibiotics used in all food-producing animals (including poultry) because they are cheap and easily available. Therefore, the widespread use of tetracyclines often leads to resistance[3] not only among pathogenic, but also commensal intestinal bacteria, such as E. coli[8], resulting in the transmission of resistant bacterial strains from poultry to humans via the food chain[9]. In E. coli, the genes tet(A), tet(B), tet(C), tet(D), and tet(E) are associated with an efflux mechanism and make an important part of the tetracycline resistance[3].It is a well-known fact that integrons play an important role in the dissemination of antimicrobial resistance among Gram-negative bacteria.…”

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Antibiotic Resistance of Escherichia Coli Isolated from Broiler Chickens

et al. 2019

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The purpose of this study was to detect the antibiotic resistance of forty-one Escherichia coli isolates from the intestinal contents of slaughtered broiler chickens using the disk diffusion method according to Kirby-Bauer. Mueller-Hinton agar plates were inoculated with 0.1 ml overnight broth cultures of individual E. coli isolates and the disks with the following concentrations of antibiotics were applied onto them: ampicillin (10 μg), cefotaxime (30 μg), gentamicin (10 μg), streptomycin (10 μg), azithromycin (15 μg), tetracycline (30 μg), ciprofloxacin (30 μg) and levofloxacin (3 μg). After the incubation at 37 °C for 16—18 hours, the inhibition zones were measured and interpreted in accordance with the Clinical and Laboratory Standard Institute (CLSI) zone diameter breakpoints. Almost all E. coli isolates showed resistance to tetracycline (92.68 %), most of them were resistant to gentamicin (75.61 %) and levofloxacine (70.73 %). Phenotypic resistance to tetracycline was further confirmed with the help of the Polymerase Chain Reaction (PCR) procedure focused on the presence of specific tet(A) and tet(B) genes. These genes were detected in all 41 E. coli isolates. On the contrary, E. coli isolates were highly susceptible to both azithromycin and streptomycin. In conclusion, the study highlighted the role of commensal E. coli bacteria isolated from the intestines of broiler chickens as an important reservoir of tetracycline resistance genes.

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Antibiotic Resistance of Escherichia Coli Isolated from Broiler Chickens

et al. 2019

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“…For this reason, the reduction of antimicrobial use at the field level throughout Europe is one of the most important aims in the poultry sector [26]. Results of this study showed Salmonella strains with a high percentage of antimicrobial resistance, especially against TET, AMP and NAL, three of the antibiotics most frequently used to treat poultry, and also used against human diseases [27,28]. Although Salmonella treatment with antibiotics is banned in the EU, its resistance to antibiotics could be acquired from different sources, such as the environment or antibiotics used to control other infections (E. coli) [29].…”

Section: Discussionmentioning

confidence: 86%

Salmonella Bacteriophage Diversity According to Most Prevalent Salmonella Serovars in Layer and Broiler Poultry Farms from Eastern Spain

Sevilla‐Navarro

Catalá-Gregori

Marín

2020

Animals

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The exploration of novel nonantibiotic interventions in the field, such as the use of bacteriophages, is necessary to avoid the presence of Salmonella. Bacteriophages are a group of viruses widely distributed in nature, strictly associated with the prokaryotic cell. Researchers have demonstrated the success of phage therapy in reducing Salmonella counts in poultry products. However, the impact that phage concentration in the environment may have against certain Salmonella serovars is not well understood. Therefore, the aim of this study was to assess Salmonella phage prevalence in commercial poultry farms in terms of the production type: layers or broilers. The most prevalent Salmonella serovars isolated in poultry production were used for phage isolation. Salmonella specific phages were isolated from 141 layer and broiler farms located in the Valencia region during 2019. Analysis of the samples revealed that 100% presented Salmonella phages, the most prevalent being against serovar S. Enteritidis (93%), followed by S. Virchow (59%), S. Typhimurium (55%), S. Infantis (52%) and S. Ohio (51%). These results indicate that poultry farms could represent an important source of Salmonella phages. Nevertheless, further studies are needed to assess the epidemiology of phages against other serovars present in other countries and their diversity from the point of view of molecular studies.

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“…This creates favorable conditions for the emergence and spread of antibioticresistant strains of microorganisms. The problem of bacterial resistance to antibacterial drugs remains relevant in the treatment of infectious diseases throughout the world, not only in veterinary medicine, but also in human medicine [3][4][5][6]. In addition, antibiotics have a negative effect on metabolic processes, the immune system, and the gastrointestinal tract of birds.…”

Section: Introductionmentioning

confidence: 99%

Biological Method for Prevention of Avian Bacterial Diseases

Лыско¹,

Zadorozhnaya²,

Suntsova³

2020

Proceedings of the International Scientific Conference the Fifth Technological Order: Prospects for the Development and Moderni

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This article is devoted to the development of a new method for the prevention of avian bacterial diseases using a probiotic at various stages of the technological cycle at a poultry farm, including managing laying hens of the parent flock, incubating and broiler rearing. We used the-Organix‖ probiotic in the experiment, which was sprayed using a cold fog generator according to the developed schemes. The use of this method reduces the total microbial background in incubators and in facilities of keeping the broiler chickens and laying hens, reduces the growth of pathogenic and opportunistic microorganisms, creates a healthy microbial background, reduces the amount of ammonia in poultry houses and the effect of harmful microorganisms on the macroorganism. This positively affects the embryonic development and postnatal developing of birds, enhances the metabolism in their body. The hatchability rate is increased by 2.7%, the viability of laying hens and broilers is increased by 1.9% and 2.8%, the intensity of egg production and meat yieldby 3.6% and 4.2%, the profitability of hatching egg and broiler productionby 7.2% and 4.5%. The developed method contributes to the production of safe poultry products without antibacterial drugs use.

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Resistance to tetracycline in Escherichia coli isolates from poultry meat: epidemiology, policy and perspective

Cited by 27 publications

References 44 publications

Antibiotic Resistance of Escherichia Coli Isolated from Broiler Chickens

Antibiotic Resistance of Escherichia Coli Isolated from Broiler Chickens

Salmonella Bacteriophage Diversity According to Most Prevalent Salmonella Serovars in Layer and Broiler Poultry Farms from Eastern Spain

Biological Method for Prevention of Avian Bacterial Diseases

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