Resolution enhancement in an extended depth of field for volumetric two-photon microscopy

He, Hongsen; Kong, Chen; Chan, Ka Yan; So, Won; Fok, Hiu Ka; Ren, Yu-Xuan; Lai, Cora Sau Wan; Tsia, Kevin K.; Wong, Kenneth K. Y.

doi:10.1364/ol.394282

Cited by 8 publications

(10 citation statements)

References 18 publications

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“…For instance, a binary concentric ring pattern overlaid on a quadratic phase pattern allows the tailoring of the axial intensity of the Bessel focus [84]. For high-order Bessel beams, a spiral phase mask is superimposed with the concentric phase mask (Figure 7D) [81,85]. The lateral shape of the high-order Bessel beam is controlled by order of the spiral phase mask (Figure 7E) [82].…”

Section: Spatial Light Modulatormentioning

confidence: 99%

“…The response of a superpixel is the sum over the individual active pixels in the target plane. The responses of adjacent pixels inside the superpixel are 2π/n 2 out of phase along the [81]. (E) Lateral beam profiles of the 0th-, 2nd-, and 4th-order Bessel beams [82].…”

Section: Spatial Light Modulatormentioning

confidence: 99%

“…(L,M) Simulated intensity distributions of single (L) and dual (M) vortex Airy beams [48]. Reproduced with permission from Springer Nature [82], reprinted with permission of OSA [80,81,83], and reprinted with the permission of AIP Publishing [48].…”

Section: Spatial Light Modulatormentioning

confidence: 99%

“…In many real applications, this bending feature would be considered a disadvantage. However, recent progress on superresolution microscopy and multiphoton microscopy suggests that the bending freedom carries the information of the depth imaging [36,81].…”

Section: Self-accelerationmentioning

confidence: 99%

“…Moreover, the resolution with both the Airy and Bessel beams would be sacrificed owing to the inhomogeneous light intensity distribution and the parasitic side lobes. To improve the transverse resolution and thus preserve the extended depth capturing ability, the side lobe from the Bessel beam could be inhibited by a higher-order Bessel beam to scan the sample and subtracting the two images [81]. In contrast to the conventional 2D switching laser modes (SLAM), such a counterpart in 3D, termed as volumetric SLAM (v-SLAM), features extended depth and improved transverse resolution (Figure 13) [81].…”

Section: Improve the Transverse Resolution Of Two-photon Microscopy With Bessel Beamsmentioning

confidence: 99%

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Non-Diffracting Light Wave: Fundamentals and Biomedical Applications

Ren

Tang

et al. 2021

Front. Phys.

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The light propagation in the medium normally experiences diffraction, dispersion, and scattering. Studying the light propagation is a century-old problem as the photons may attenuate and wander. We start from the fundamental concepts of the non-diffracting beams, and examples of the non-diffracting beams include but are not limited to the Bessel beam, Airy beam, and Mathieu beam. Then, we discuss the biomedical applications of the non-diffracting beams, focusing on linear and nonlinear imaging, e.g., light-sheet fluorescence microscopy and two-photon fluorescence microscopy. The non-diffracting photons may provide scattering resilient imaging and fast speed in the volumetric two-photon fluorescence microscopy. The non-diffracting Bessel beam and the Airy beam have been successfully used in volumetric imaging applications with faster speed since a single 2D scan provides information in the whole volume that adopted 3D scan in traditional scanning microscopy. This is a significant advancement in imaging applications with sparse sample structures, especially in neuron imaging. Moreover, the fine axial resolution is enabled by the self-accelerating Airy beams combined with deep learning algorithms. These additional features to the existing microscopy directly realize a great advantage over the field, especially for recording the ultrafast neuronal activities, including the calcium voltage signal recording. Nonetheless, with the illumination of dual Bessel beams at non-identical orders, the transverse resolution can also be improved by the concept of image subtraction, which would provide clearer images in neuronal imaging.

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Section: Spatial Light Modulatormentioning

confidence: 99%

Section: Spatial Light Modulatormentioning

confidence: 99%

Section: Spatial Light Modulatormentioning

confidence: 99%

Section: Self-accelerationmentioning

confidence: 99%

Section: Improve the Transverse Resolution Of Two-photon Microscopy With Bessel Beamsmentioning

confidence: 99%

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Non-Diffracting Light Wave: Fundamentals and Biomedical Applications

Ren

Tang

et al. 2021

Front. Phys.

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Small focal spot formation by vector beams

Kozawa

Sato

2021

Progress in Optics

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Tomographic-Encoded Multiphoton Microscopy

Dong

Ren

et al. 2022

ACS Photonics

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Axial scanning in multiphoton microscopy (MPM) is typically realized by mechanically shifting either the objective or the sample. However, the scan speed is usually hindered by the mechanical inertia of the bulky mass. Although the extended depth of field provided by the non-diffracting beam allows fast volumetric imaging, it abandons the axial resolution. Here, we demonstrate a novel and powerful tomographic technique using the Bessel droplet in MPM, termed tomographic-encoded multiphoton (TEMP) microscopy, for structural illumination in the axial direction. We show that benefiting from the high-order nonlinear excitation in MPM, the side-lobe cancellation and smaller beam focus of the Bessel droplet realize better image quality. TEMP microscopy allows fast axial scanning, less risks of photodamage and photobleaching, and high-resolution and high-contrast imaging. Furthermore, fewer raw images are required for the 3D image reconstruction. To demonstrate its usability and advantages for scattering tissues and biomedical applications, we showcase TEMP microscopy with highly scattering fluorescence microspheres and mouse brain slice. More details can be visualized by the Bessel droplet compared with the conventional Gaussian and Bessel beam. The depth-resolving performance of the Bessel droplet has an excellent consistency with the Gaussian beam. More importantly, the TEMP technique is an easy-plug-in method for the current microscopy system. TEMP microscopy is promising for fast volumetric multiphoton imaging, especially for highly scattering tissues.

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Resolution enhancement in an extended depth of field for volumetric two-photon microscopy

Cited by 8 publications

References 18 publications

Non-Diffracting Light Wave: Fundamentals and Biomedical Applications

Non-Diffracting Light Wave: Fundamentals and Biomedical Applications

Small focal spot formation by vector beams

Tomographic-Encoded Multiphoton Microscopy

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