Resolution of RNA using high-performance liquid chromatography

Ferrocene-containing nucleic acids. Synthesis and electrochemical properties

“…High-performance liquid chromatography with UV detection is a popular conventional procedure for purification and analysis of NA 95,96 owing to its high efficiency and a relatively low cost. Wlassoff and King 50 have carried out a comparison of various UV and electrochemical techniques for detecting ferrocene-containing oligonucleotides to demonstrate that the latter enable quantitative determination of DNA in femtomolar concentrations, while the sensitivity of the optical methods does not exceed picomolar concentrations (Table 3), i.e., it is by about three orders of magnitude lower.…”