Resolvin D5, a Lipid Mediator, Inhibits Production of Interleukin-6 and CCL5 Via the ERK-NF- ¥ éSignaling Pathway in Lipopolysaccharide-Stimulated THP-1 Cells

Abstract

One of the omega-3 essential fatty acids, docosahexaenoic acid (DHA), is a significant constituent of the cell membrane and the precursor of several potent lipid mediators. These mediators are considered to be important in preventing or treating several diseases. Resolvin D5, an oxidized lipid mediator derived from DHA, has been known to exert anti-inflammatory effects. However, the detailed mechanism underlying these effects has not yet been elucidated in human monocytic THP-1 cells. In the present study, we investigated the effects of resolvin D5 on inflammation-related signaling pathways, including the extracellular signal-regulated kinase (ERK)-nuclear factor (NF)-κB signaling pathway. Resolvin D5 downregulated the production of interleukin (IL)-6 and chemokine (C-C motif) ligand 5 (CCL5). Additionally, these inhibitory effects were found to be modulated by mitogen-activated protein kinase (MAPK) and NF-κB in lipopolysaccharide (LPS)-treated THP-1 cells. Resolvin D5 inhibited the LPS-stimulated phosphorylation of ERK and translocation of p65 and p50 into the nucleus, resulting in the inhibition of IL-6 and CCL5 production. These results revealed that resolvin D5 exerts anti-inflammatory effects in LPS-treated THP-1 cells by regulating the phosphorylation of ERK and nuclear translocation of NF-κB.

Daily Eicosapentaenoic Acid Infusion in IUGR Fetal Lambs Reduced Systemic Inflammation, Increased Muscle ADRβ2 Content, and Improved Myoblast Function and Muscle Growth

“…In contrast, concentrations of other white blood cells remained elevated, indicating that the anti-inflammatory effects were primarily directed at monocytes. Cell culture studies show that ω-3 PUFA moderates monocytic activity by inhibiting canonical NFκB, p38 MAPK, and TLR4 pathways [ 65 , 66 , 67 ], which in turn reduce cytokine production and secretion [ 68 ]. Similar outcomes were reported when ω-3 PUFA was used to treat chronic inflammatory conditions in humans, as monocytic populations and activity were suppressed with little or no effect on other leukocytes [ 69 , 70 , 71 ].…”

Matrisomal components involved in regenerative wound healing in axolotl and Acomys: implications for biomaterial development

“…94 Resolvin D5, an oxidized lipid mediator, has anti-inflammatory properties by mediating immune cell behavior and recent work indicated this agent has a direct role during re-epithelialization. 95,96 Finally, retinoic acid (a vitamin A metabolite) has been found in regenerating zebrafish tissues 97 and it was shown to enhance the production of ECM components during wound healing. 98 In another study, full-thickness skin wounds of 12 mm diameter were inflicted on the backs of fetal sheep.…”

Dysosmobacter welbionis effects on glucose, lipid, and energy metabolism are associated with specific bioactive lipids

“…People with T2D mellitus are at increased risk for depression ( 48 ). The second lipid markedly affected was the RvD5, a lipid known as proresolving mediator in intestinal inflammation ( 25 , 49 ). In our study RvD5 was decreased by the HFD and strongly increased by D. welbionis J115 T treatment.…”