Resolving the CaP-bone interface

Grandfield, Kathryn; Palmquist, Anders; Engqvist, Håkan; Thomsen, Peter

doi:10.4161/biom.20062

Cited by 10 publications

(5 citation statements)

References 69 publications

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“…228 This technique integrates a scanning electron beam and a second, focused ion beam (FIB) in the same microscope. The ion beam, typically using accelerated, ion scan, performs site-specific removal (or milling) of thin layers of resin-embedded or cryo-prepared samples.…”

Section: Identification Of Subcellular Compartmentsmentioning

confidence: 99%

“…Focused ion beam/scanning electron microscopy (FIB/SEM), sometimes called dual beam or ion abrasion electron microscopy, is another approach for providing 3D structural details of cells and tissue . This technique integrates a scanning electron beam and a second, focused ion beam (FIB) in the same microscope.…”

Section: Identification Of Subcellular Compartmentsmentioning

confidence: 99%

“…227 Focused ion beam/scanning electron microscopy (FIB/ SEM), sometimes called dual beam or ion abrasion electron microscopy, is another approach for providing 3D structural details of cells and tissue. 228 This technique integrates a scanning electron beam and a second, focused ion beam (FIB) in the same microscope. The ion beam, typically using accelerated, ion scan, performs site-specific removal (or milling) of thin layers of resin-embedded or cryo-prepared samples.…”

Section: Molecular Markersmentioning

confidence: 99%

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Bioanalysis of Eukaryotic Organelles

et al. 2013

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Section: Identification Of Subcellular Compartmentsmentioning

confidence: 99%

Section: Identification Of Subcellular Compartmentsmentioning

confidence: 99%

Section: Molecular Markersmentioning

confidence: 99%

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Bioanalysis of Eukaryotic Organelles

et al. 2013

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“…However, this is a time‐ and labor‐intensive process, and few uptake events may be observed in an individual sample, consisting as it does of an ultrathin section (typically 30–100 nm, rarely exceeding 150 nm, as electrons less readily pass through biological material of this thickness) . Additionally, artifacts may be introduced into TEM samples due to damage during the sectioning process, which can be a particular problem for the study of cellular interactions with synthetic materials, as the interface between “soft” biological material and “hard” synthetic particles can be altered …”

Section: Resultsmentioning

confidence: 99%

Early Membrane Responses to Magnetic Particles are Predictors of Particle Uptake in Neural Stem Cells

Fernandes

Adams

Furness

et al. 2015

Part & Part Syst Charact

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Magnetic particles (MPs) offer several advantages for neural cell therapy, but limited particle uptake by neural cells is a barrier to translation. It is recently proved that tailoring particle physicochemical properties (by enhancing their iron content) dramatically improves uptake in neural stem cells (NSCs)—a major transplant population. High‐throughput screening of particles with varying physicochemical properties can therefore aid in identifying particles with optimal uptake features, but research is hampered by the lack of simple methodologies for studying neural cell membrane responses to nanoparticle platforms. A high‐resolution–high throughput method has been used to study early membrane responses of primary rodent NSCs to particles of variant magnetite loading, to attempt to correlate these responses with known particle internalization profiles. Membrane imaging is enhanced through sequential staining with osmium (O) and thiocarbohydrazide (T), a method termed OTOTO, combined with field‐emission scanning electron microscopy (FESEM). A five‐point classification system was used to systematically evaluate early MP‐induced membrane responses to particles possessing distinct physicochemical properties. Significantly different profiles of membrane activation were noted that correlate with particle uptake profiles. It is suggested that our method can serve as a valuable predictor of particle internalization in neural cells for diverse particle platforms.

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“…Many studies have described the biological activity mechanisms of bioresorbable ceramics in detail [ 56 , 89 , 90 , 91 , 92 , 93 , 94 , 95 ]. In brief, after bioresorbable ceramics are implanted, a layer of nano-apatite crystals formed on the surface of the CaP ceramics absorbs various osteogenic proteins, particularly small molecular proteins (e.g., growth factors) [ 96 , 97 , 98 , 99 ].…”

Section: U-ha/plla An Outstanding Bioresorbable Compositementioning

confidence: 99%

A Narrative Review of u-HA/PLLA, a Bioactive Resorbable Reconstruction Material: Applications in Oral and Maxillofacial Surgery

et al. 2021

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The advent of bioresorbable materials to overcome limitations and replace traditional bone-reconstruction titanium-plate systems for bone fixation, thus achieving greater efficiency and safety in medical and dental applications, has ushered in a new era in biomaterial development. Because of its bioactive osteoconductive ability and biocompatibility, the forged composite of uncalcined/unsintered hydroxyapatite and poly L-lactic acid (u-HA/PLLA) has attracted considerable interest from researchers in bone tissue engineering, as well as from clinicians, particularly for applications in maxillofacial reconstructive surgery. Thus, various in vitro studies, in vivo studies, and clinical trials have been conducted to investigate the feasibility and weaknesses of this biomaterial in oral and maxillofacial surgery. Various technical improvements have been proposed to optimize its advantages and limit its disadvantages. This narrative review presents an up-to-date, comprehensive review of u-HA/PLLA, a bioactive osteoconductive and bioresorbable bone-reconstruction and -fixation material, in the context of oral and maxillofacial surgery, notably maxillofacial trauma, orthognathic surgery, and maxillofacial reconstruction. It simultaneously introduces new trends in the development of bioresorbable materials that could used in this field. Various studies have shown the superiority of u-HA/PLLA, a third-generation bioresorbable biomaterial with high mechanical strength, biocompatibility, and bioactive osteoconductivity, compared to other bioresorbable materials. Future developments may focus on controlling its bioactivity and biodegradation rate and enhancing its mechanical strength.

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Resolving the CaP-bone interface

Cited by 10 publications

References 69 publications

Bioanalysis of Eukaryotic Organelles

Bioanalysis of Eukaryotic Organelles

Early Membrane Responses to Magnetic Particles are Predictors of Particle Uptake in Neural Stem Cells

A Narrative Review of u-HA/PLLA, a Bioactive Resorbable Reconstruction Material: Applications in Oral and Maxillofacial Surgery

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