Respiration and Phosphorylation of Mitochondria from Normal and Crown-Gall Tissue Cultures of Tomato

Metastasis involves the spread of cancer cells from the primary tumor to surrounding tissues and to distant organs and is the primary cause of cancer morbidity and mortality. In order to complete the metastatic cascade, cancer cells must detach from the primary tumor, intravasate into the circulatory and lymphatic systems, evade immune attack, extravasate at distant capillary beds, and invade and proliferate in distant organs. Currently, several hypotheses have been advanced to explain the origin of cancer metastasis. These involve an epithelial mesenchymal transition, an accumulation of mutations in stem cells, a macrophage facilitation process, and a macrophage origin involving either transformation or fusion hybridization with neoplastic cells. Many of the properties of metastatic cancer cells are also seen in normal macrophages. A macrophage origin of metastasis can also explain the long-standing “seed and soil” hypothesis and the absence of metastasis in plant cancers. The view of metastasis as a macrophage metabolic disease can provide novel insight for therapeutic management.

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“…209 Indeed, defects in mitochondrial morphology and energy metabolism were later described in crown-gall tumors. 212-214 …”

Section: The Absence Of Metastases In Crown-gall Plant Tumorsmentioning

confidence: 99%

On the Origin of Cancer Metastasis

2013

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“…Since the respiratory rate of the parent plant tissue is, in most cases, limited by oxidative phosphorylation and therefore stimulated by DNP, the property of respiratory control in the mitochondria prepared from such tissue is essential for critical investigations. However, a mere stimulation of mitochondrial oxidations by ADP (14,16,28) is not necessarily indicative of respiratory control (8). This effect may be due to a low level of endogenous adenylate (and may be indicative of coupling) or to other reactions not related to respiratory control (29).…”

Section: Methodsmentioning

confidence: 99%

Metabolic Processes in Cytoplasmic Particles of the Avocado Fruit. VI. Controlled Oxidations and Coupled Phosphorylations

1964

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“…The inadequacies of inhibitor studies have been stressed (22). In vitro cultures of normal and tumor tomato tissues were studied by Tamaoki and coworkers (36) who showed that mitochondria isolated from such materials possessed the ability to oxidize tricarboxylic acid cycle intermediates and to perform oxidative phosphorylations. Both the rate of oxidation and the P/O ratios were less in mitochondria isolated from tumors. A different approach to investigating respiratory pathways in tumor tissues was adopted in the present work.…”

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confidence: 99%

Pathways of Respiration in Plant Tumors

Scott

Smillie²

1964

Plant Physiol.

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Altered pathways of respiration in plants after infection with fungi, particularly obligate fungal pathogens, are well documented (27), but little is known about plant respiration after infection by bacterial pathogens. Agrobacterium tumefaciens, a bacterial pathogen with a wide plant host range, has the ability to permanently alter host cells to tumor cells (9). Information on the respiratory pathways of these altered cells is of interest to plant pathologists for its possible significance in over-all disease symptoms and to students of oncology.The following reports have appeared in the literature on respiratory pathways of these tissues. Brucher and Schmidt (11) added inhibitors of glycolysis and the tricarboxylic acid cycle to in vitro grown normal and tumor tissues of Datura and carrot and concluded that the importance of respiration via glycolyis and the tricarboxylic acid cycle is reduced in tumor tissues. The inadequacies of inhibitor studies have been stressed (22). In vitro cultures of normal and tumor tomato tissues were studied by Tamaoki and coworkers (36) who showed that mitochondria isolated from such materials possessed the ability to oxidize tricarboxylic acid cycle intermediates and to perform oxidative phosphorylations. Both the rate of oxidation and the P/O ratios were less in mitochondria isolated from tumors.A different approach to investigating respiratory pathways in tumor tissues was adopted in the present work. The activities of enzymes of the hexose monophosphate shunt, glycolysis, and the tricarboxylic acid cycle were determined in homogenates of normal and tumor tissues of red beet roots. In addition, the dissimilation of glucose specifically labeled in the -1, -2, or -6 position was examined in these tissues. The results obtained are discussed in relation to the relative importance of the hexose monophosphate shunt and glycolysis in both tissues. '

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Respiration and Phosphorylation of Mitochondria from Normal and Crown-Gall Tissue Cultures of Tomato

Cited by 17 publications

References 29 publications

On the Origin of Cancer Metastasis

On the Origin of Cancer Metastasis

Metabolic Processes in Cytoplasmic Particles of the Avocado Fruit. VI. Controlled Oxidations and Coupled Phosphorylations

Pathways of Respiration in Plant Tumors

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