Respiratory apparatus

Navarro, Miguel; Ruberte, J.; Carretero, A.

doi:10.1016/b978-0-12-812972-2.50006-4

Cited by 9 publications

(7 citation statements)

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“…For example, fluid transport is characteristic of many organs and plays an important role in epithelium development and function [26],[27] . Our engineered airway MOA tubes, of a size similar to the mouse trachea (1-1.5 mm inner diameter [28] ), were perfusable and exhibited cyclic lumen expansion and relaxation, in synchronization with a peristaltic input flow of culture medium ( Figure 5 a, Video S5, Supporting information). Such a flow-able system ( Figure S7 a-b, Supporting information) is an initial step for recreating fluid transportation in large engineered organ tubes, where the lumen is accessible within a closed, controllable system separated from the environment on the basal side.…”

Section: Resultsmentioning

confidence: 99%

Section: Downstream Processing Of Moas For Tissue Engineering Applicamentioning

confidence: 99%

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Bioassemblying Macro-Scale, Lumnized Airway Tubes of Defined Shape via Multi-Organoid Patterning and Fusion

Liu

Dabrowska

Mavousian

et al. 2020

Preprint

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Epithelial, stem-cell derived organoids are ideal building blocks for tissue engineering, however, scalable and shape-controlled bioassembly of epithelial organoids into larger and anatomical structures has yet to be achieved. Here, a robust organoid engineering approach, Multi-Organoid Patterning and Fusion (MOrPF), is presented to assemble individual airway organoids of different sizes into upscaled, scaffold-free airway tubes with pre-defined shapes. Multi-Organoid Aggregates (MOAs) undergo accelerated fusion in a matrix-depleted, free-floating environment, possess a continuous lumen and maintain prescribed shapes without an exogenous scaffold interface. MOAs in the floating culture exhibit a well-defined three-stage process of inter-organoid surface integration, luminal material clearance and lumina connection. The observed shape stability of patterned MOAs is confirmed by theoretical modelling based on organoid morphology and the physical forces involved in organoid fusion. Immunofluorescent characterization shows that fused MOA tubes possess an unstratified epithelium consisting mainly of tracheal basal stem cells. By generating large, shape-controllable organ tubes, MOrPF enables upscaled organoid engineering towards integrated organoid-devices and structurally complex organ tubes.

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Section: Resultsmentioning

confidence: 99%

Section: Downstream Processing Of Moas For Tissue Engineering Applicamentioning

confidence: 99%

Bioassemblying Macro-Scale, Lumnized Airway Tubes of Defined Shape via Multi-Organoid Patterning and Fusion

Liu

Dabrowska

Mavousian

et al. 2020

Preprint

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“…Two regions-of-interest (ROI: cartilage and noncartilage) were manually segmented using the NAD(P)H intensity image, in which the tracheal hyaline cartilage is visible. 10 This mask was then applied to the corresponding NAD(P)H, FAD, and redox ratio image time series (ImageJ). The mean value was calculated for each ROI per image.…”

Section: Redox Imaging and Optical Coherence Tomography Of The Respirmentioning

confidence: 99%

“…16 Therefore, we attribute this increased change in redox ratio of cartilage regions to the additional metabolically active cells (chondrocytes) present below the epithelia of the cartilage regions that are not present in noncartilage regions. 10 Finally, we compared the change in cellular metabolism and cilia-driven fluid flow caused by cyanide treatment. These results indicate that, when oxidative phosphorylation is inhibited in ciliated epithelial cells with cyanide (increased redox ratio), there is reduced capacity to generate effective (i.e., þX velocity) cilia-driven fluid flow.…”

Section: Redox Imaging and Optical Coherence Tomography Of The Respirmentioning

confidence: 99%

Redox imaging and optical coherence tomography of the respiratory ciliated epithelium

et al. 2019

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Optical coherence tomography (OCT) is an emerging technology for in vivo airway and lung imaging. However, OCT lacks sensitivity to the metabolic changes caused by inflammation, which drives chronic respiratory diseases such as asthma and chronic obstructive pulmonary disorder. Redox imaging (RI) is a label-free technique that uses the autofluorescence of the metabolic coenzymes NAD(P)H and flavin adenine dinucleotide (FAD) to probe cellular metabolism and could provide complimentary information to OCT for airway and lung imaging. We demonstrate OCT and RI of respiratory ciliated epithelial function in ex vivo mouse tracheae. We applied RI to measure cellular metabolism via the redox ratio [intensity of NAD(P)H divided by FAD] and particle tracking velocimetry OCT to quantify cilia-driven fluid flow. To model mitochondrial dysfunction, a key aspect of the inflammatory process, cyanide was used to inhibit oxidative metabolism and reduce ciliary motility. Cyanide exposure over 20 min significantly increased the redox ratio and reversed cilia-driven fluid flow. We propose that RI provides complementary information to OCT to assess inflammation in the airway and lungs. © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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“…The vocal folds are located in the vestibule of the larynx, where there is also a large laryngeal recess. It is when air passes during expiration generating vibration of the vocal folds that sound is emitted ( Navarro et al, 2017a ).…”

Section: Introductionmentioning

confidence: 99%

From Mating to Milk Access: A Review of Reproductive Vocal Communication in Mice

Capas-Peneda

Torres

Olsson

2022

Front. Behav. Neurosci.

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Vocalisations play a central role in rodent communication, especially in reproduction related behaviours. In adult mice (Mus musculus) the emission of ultrasonic vocalisations (USVs) has been observed in courtship and mating behaviour, especially by males. These have been found to have distinctive individual signatures that influence female choice of mating partner. The most recent findings show that vocal communication also has a role in parental cooperation, in that female mice communicate with male partners in ultrasonic frequencies to induce paternal behaviour. Infant vocalisations form the other important part of reproductive vocal communication. Although born deaf, neonatal mice are capable of producing vocalisations since birth. As an altricial species, successful mother-infant communication is essential for survival, and these vocalisations are important modulators of maternal behaviour. Three main types of infant vocalisations have been identified and characterised. Most research has addressed pure USVs, related to stressful situations (e.g., cold, isolation, handling, presence of unfamiliar males or predators), which usually elicit maternal search and retrieval. In addition, broad-band spectrum signals, emitted post-partum during cleaning of foetal membranes, inhibit biting and injury by adults and “wriggling calls,” emitted during suckling, release maternal behaviour (such as licking). Several variables have been identified to modulate vocalisations in mice, including individual characteristics such as strain/genotype, age, sex, and experimental factors such as pharmacological compounds and social context. In recent years, there has been a big increase in the knowledge about the characteristics of vocal communication in rodents due to recent technological advances as well as a growing interest from the neuroscience community. Vocalisation analysis has become an essential tool for phenotyping and evaluating emotional states. In this review, we will (i) provide a comprehensive summary of the current knowledge on mouse reproductive vocal communication and (ii) discuss the most recent findings in order to provide a broad overview on this topic.

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Respiratory apparatus

Cited by 9 publications

References 0 publications

Bioassemblying Macro-Scale, Lumnized Airway Tubes of Defined Shape via Multi-Organoid Patterning and Fusion

Bioassemblying Macro-Scale, Lumnized Airway Tubes of Defined Shape via Multi-Organoid Patterning and Fusion

Redox imaging and optical coherence tomography of the respiratory ciliated epithelium

From Mating to Milk Access: A Review of Reproductive Vocal Communication in Mice

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