Respiratory burst reaction changes with age in rat peritoneal macrophages

Alvarez, Eloísa; Marı́a, Consuelo Santa; Machado, Alberto

doi:10.1016/0167-4889(93)90079-5

Cited by 18 publications

(14 citation statements)

References 38 publications

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“…A study showed that aged rats treated with L-CAR exhibited improvement in the levels of SOD, catalase, and GPx [46]. The increased SOD activity seen on treatment could be because of the anti-radical effects of L-CAR and its antioxidant role as a metal chelator [53], and the increase in catalase activity upon L-CAR administration may be attributed to the improved activity of glucose-6-phosphate dehydrogenase (G6PD) that generates NADPH which is required for the regeneration of catalase activity [8]. Also, L-CAR can protect the endogenous antioxidant defense system activities, including GPx, catalase and SOD from peroxidative damage [16,34].…”

Section: Discussionmentioning

confidence: 93%

L-carnitine prevents memory impairment induced by chronic REM-sleep deprivation

Alzoubi

Rababa’h

Owaisi

et al. 2017

Brain Research Bulletin

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Section: Discussionmentioning

confidence: 93%

L-carnitine prevents memory impairment induced by chronic REM-sleep deprivation

Alzoubi

Rababa’h

Owaisi

et al. 2017

Brain Research Bulletin

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“…Previously we provided evidence that respiratory burst reaction decreases with age in rat peritoneal macrophages, using PMA and N‐FMLP as stimuli [8,9]. The NADPH oxidase activity did not vary with age in these cells [10].…”

Section: Introductionmentioning

confidence: 84%

Age-related changes in membrane lipid composition, fluidity and respiratory burst in rat peritoneal neutrophils

Alvarez¹,

Ruı́z-Gutı́errez

Sobrino

et al. 2001

Clinical and Experimental Immunology

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The O2*(-) production has been studied in rat peritoneal neutrophils of different age (3, 12 and 24 months), in order to analyse whether the neutrophil respiratory burst is modified with increasing age. To stimulate NADPH oxidase, the enzyme responsible for the respiratory burst, two stimuli that act in different way have been used: phorbol myristate acetate (PMA) and N-formyl-methionyl-leucyl-phenylalanine (N-FMLP). Production of O2*(-) decreased with age in neutrophils stimulated with N-FMLP (about 40%), but not in the stimulated with PMA. No difference in NADPH oxidase activity was found with age. The NADPH is supplied to the respiratory burst mainly by the pentose phosphate shunt. A progressive and significant decrease in the two most important enzymes of this route, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase, was detected as a function of age; in spite of this reduction, the NADPH produced by cells from old animals seems not limiting for the O2*(-) production. The N-FMLP-induced decrease in the O2*(-) production may be related to the age-dependent increase in the membrane fluidity observed. A decline in the cholesterol/phospholipid ratio and a rise in the total polyunsaturated fatty acids content were found, that correlated well with the increase in the membrane fluidity. The decrease (50%) of phosphatidylinositols in the 24-month-old animals may be also related to the age-impairment in the respiratory burst found after stimulation with N-FMLP. These studies suggest that the age-related alterations in neutrophil may result in diminished neutrophil function and increased susceptibility to infection in the ageing.

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“…After 7 days removed from astrocytic influences, the microglia were essentially macrophages, and secreting the toxic molecules associated with macrophages, as demonstrated by Slepko & Levi (1996). Thirdly we stimulated further respiratory burst in the cells with FMLP or Zymosan A. FMLP is a widely used macrophage activator which stimulates chemotaxis and a powerful respiratory burst reaction (Pick 1986; Alvarez et al . 1993).…”

Section: Discussionmentioning

confidence: 99%

The effect of microglia on embryonic dopaminergic neuronal survival in vitro: diffusible signals from neurons and glia change microglia from neurotoxic to neuroprotective

Zietlow

Dunnett

Fawcett

1999

Eur J of Neuroscience

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When embryonic dopaminergic neurons are transplanted into the adult brain, approximately 95% die within a few days. To assess whether microglia activated during transplantation might be responsible for this rapid death, we examined the effect of microglia on rat embryonic dopaminergic neurons in vitro. Conditioned medium from 7-day-old microglia was found to decrease the number of dopamine neurons surviving in primary culture, but activation of the microglia with N-formyl-methionyl-leucyl-phenylalanine (FMLP) or Zymosan A did not increase the toxicity of the conditioned medium. We next tested the effect of coculturing microglia and dopaminergic neurons by placing microglia in semipermeable well inserts over the neuronal cultures. The presence of microglia now increased dopaminergic neuronal survival, microglial activation again having no effect. To increase yet further the possible interactions between microglia and neurons, the mesencephalic cells and microglia were mixed together and placed as a tissue in three-dimensional culture, and here again the presence of microglia increased dopaminergic neuronal survival with no effect of activation. Contact of microglia with the mesencephalic cells therefore converted them from being toxic to dopaminergic neurons to promoting their survival. The change in microglial effect from toxic to protective was caused by soluble molecules secreted by cells in the neuronal cultures, as conditioned medium derived from microglia-neuronal cocultures also had a dopaminergic neuron survival effect, indicating that microglia in cocultures behave differently from microglia removed from neuronal and glial influence. Microglia cocultured with either neurons or astrocytes downregulated inducible nitric oxide synthase (iNOS), indicating a decrease in the production of nitric oxide and possibly other toxic molecules. These findings indicate that in their natural environment, microglia are likely to be beneficial for the survival of embryonic dopaminergic grafts.

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Respiratory burst reaction changes with age in rat peritoneal macrophages

Cited by 18 publications

References 38 publications

L-carnitine prevents memory impairment induced by chronic REM-sleep deprivation

L-carnitine prevents memory impairment induced by chronic REM-sleep deprivation

Age-related changes in membrane lipid composition, fluidity and respiratory burst in rat peritoneal neutrophils

The effect of microglia on embryonic dopaminergic neuronal survival in vitro: diffusible signals from neurons and glia change microglia from neurotoxic to neuroprotective

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