Respiratory changes in a murine model of spontaneous systemic lupus erythematosus

Souza-Fernandes, Alba B.; Rocco, Patricia R. M.; Contador, Renata S.; Menezes, Sara L. S.; Faffe, Débora S.; Negri, Elnara M.; Capelozzi, Vera Luíza; Zin, Walter A.

doi:10.1016/j.resp.2005.09.005

Cited by 4 publications

(3 citation statements)

References 40 publications

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“…[15][16][17][18] Murine SLE models also exhibit thoracic manifestations. 19 The MRL/MpJ-lpr mouse model carries the lymphoproliferation (lpr) mutation in the apoptosisrelated Fas gene, resulting in autoreactive lymphocyte proliferation. This mouse model, which closely mimics SLE in humans, exhibits lymphadenopathy, splenomegaly and hypergammaglobulinaemia with anti-doublestranded DNA (anti-dsDNA) antibodies leading to tissue deposition and injury in various organs, including the lung and kidney.…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of mediastinal fat‐associated lymphoid cluster development and lung cellular infiltration in murine autoimmune disease models and the corresponding normal control strains

2015

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SummaryWe previously discovered mediastinal fat-associated lymphoid clusters (MFALCs) as novel lymphoid clusters associated with mediastinal fat tissue in healthy mice. However, no data about their morphology in immune-associated disease conditions, and their relationship with lung infiltration, is available to date. In the present study, we compared the morphological features of MFALCs in 4-month-old male murine autoimmune disease models (MRL/MpJ-lpr mice and BXSB/MpJ-Yaa mice) with those of the corresponding control strains (MRL/MpJ and BXSB/MpJ, respectively). In addition, we analysed their correlation with lung infiltration. Furthermore, immunohistochemistry for CD3, B220, Iba1, Gr1 and BrdU was performed to detect T cells and B cells, macrophages, granulocytes and proliferating cells, respectively. The spleen weight to body weight ratios and anti-double-stranded DNA autoantibody titres were found to be significantly higher in the autoimmune models than in the control strains. Furthermore, the autoimmune model presented prominent MFALCs, with a significantly greater ratio of lymphoid cluster area to total mediastinal fat tissue area, and more apparent diffused cellular infiltration into the lung lobes than the other studied strains. Higher numbers of T and B cells, macrophages and proliferating cells, but fewer granulocytes, were observed in the autoimmune models than in the control strains. Interestingly, a significant positive Pearson's correlation between the size of the MFALCs and the density of CD3-, B220-and Iba1-positive cells in the lung was observed. Therefore, our data suggest a potentially important role for MFALCs in the progression of lung disease. However, further investigation is required to clarify the pathological role of MFALCs in lung disease, especially in inflammatory disorders.

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Section: Introductionmentioning

confidence: 99%

Comparative analysis of mediastinal fat‐associated lymphoid cluster development and lung cellular infiltration in murine autoimmune disease models and the corresponding normal control strains

2015

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“…NZW mice themselves are normally clinically healthy, but they still present subclinical features of SLE such as nephritis, increased susceptibility to environmental insults 10 and to immune‐mediated end‐organ damage 13 . In addition, these animals have been shown to present a deficiency in eosinophil peroxidase (EPO), 14,15 abnormal inflammatory responses to intraperitoneal insult, 16 and altered levels of pro‐angiogenic and pro‐fibrogenic factors 15,17 …”

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confidence: 99%

“…13 In addition, these animals have been shown to present a deficiency in eosinophil peroxidase (EPO), 14,15 abnormal inflammatory responses to intraperitoneal insult, 16 and altered levels of pro-angiogenic and pro-fibrogenic factors. 15,17 In view of the overall susceptibility of NZW mice to develop chronic inflammatory conditions and bearing in mind the association between angiogenesis, it was reasonable to suppose that this strain of mice might also display aberrant wound healing responses. As this type of analysis had not been carried out using the same model in lupus-prone (NZW) and non-lupus prone mice (Balb/c), we set out to analyze the kinetics of the sponge-induced healing response in these animals.…”

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Mechanisms of wound healing responses in lupus‐prone New Zealand White mouse strain

Campos

Bakhle

Andrade

2008

Wound Repair Regeneration

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Host responses to synthetic implants are analogous to healing, the process of repair that follows injury. Normally, the processes of wound healing follow well-established patterns but conditions such as autoimmune diseases profoundly affect tissue repair. We have analyzed sponge-induced wound healing responses in lupus-prone New Zealand White and control (Balb/c) mouse strains by measuring inflammation, extracellular matrix deposition, angiogenesis, and cytokine production in polyether-polyurethane sponge implanted subcutaneously in male mice of these two strains. Although there was no difference in the gross appearance of the implants, further analysis of the wound healing responses, induced from 7 to 21 days post implantation, disclosed important differences between the New Zealand White and Balb/c strains. The intensity of inflammation (circulating tumor necrosis factor-alpha and inflammatory leukocytes levels) was lower but implant fibrosis (collagen and transforming growth factor-beta1) was higher in New Zealand White, compared with Balb/c mice. Angiogenesis (hemoglobin, vascular endothelial growth factor, and vascularity) in New Zealand White implants peaked earlier than in Balb/c mice. In conclusion, we have shown that wound healing responses are clearly different in this strain of lupus-prone mice and suggest that this pattern of repair was critically influenced by impaired inflammation and accelerated angiogenesis in the New Zealand White strain.

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Quantitative morphology of the lung and its importance in investigations of pulmonary diseases in mice

Klein

2019

Drug Discovery Today: Disease Models

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Respiratory changes in a murine model of spontaneous systemic lupus erythematosus

Cited by 4 publications

References 40 publications

Comparative analysis of mediastinal fat‐associated lymphoid cluster development and lung cellular infiltration in murine autoimmune disease models and the corresponding normal control strains

Comparative analysis of mediastinal fat‐associated lymphoid cluster development and lung cellular infiltration in murine autoimmune disease models and the corresponding normal control strains

Mechanisms of wound healing responses in lupus‐prone New Zealand White mouse strain

Quantitative morphology of the lung and its importance in investigations of pulmonary diseases in mice

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