Respiratory epithelial cell culture models for evaluation of ion and drug transport

Mathias, Neil; Yamashita, Fumiyoshi; Lee, Vincent H.L.

doi:10.1016/s0169-409x(96)00420-6

Cited by 59 publications

(30 citation statements)

References 215 publications

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“…199,200 Drug delivery via inhalation may be absorbed throughout the conducting airway from the trachea down to the terminal bronchioles and ultimately the distal alveoli. 201 The airway and alveolar epithelium of the lung, which have different cell types, provide barrier capability to drug absorption.…”

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confidence: 99%

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Nanomedicine in pulmonary delivery

Mansour¹,

Rhee²,

Wu³

2009

IJN

409

269

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confidence: 99%

“…[201][202][203][204][205] The cell lines used include A427, A549, HBE14o, and the Calu line (-1, -2, and -3). [205][206][207][208][209][210] These are immortal (continuous) cell lines and hence have different membrane structural features compared with mortal cell lines which influence drug absorption and efflux.…”

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confidence: 99%

Nanomedicine in pulmonary delivery

Mansour¹,

Rhee²,

Wu³

2009

IJN

409

269

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“…For bronchiolar delivery, the Calu-3 carcinoma-derived cell line has been developed and validated as an appropriate model for the study of drug adsorption and uptake across the lung epithelium (10)(11)(12)(13)(14)(15)(16). Calu-3 cells possess a wide range of influx and efflux proteins (17), which have been shown to actively transport small-molecule solutes across the pulmonary epithelium (16,18).…”

Section: Introductionmentioning

confidence: 99%

Modification of Disodium Cromoglycate Passage Across Lung Epithelium In Vitro Via Incorporation into Polymeric Microparticles

Haghi

Salama

Traini

et al. 2011

AAPS J

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Abstract. Two microparticle systems containing disodium cromoglycate (DSCG) alone or with polyvinyl alcohol (DSCG/PVA) were produced via spray drying and compared in terms of their physicochemical characteristics, aerosol performance and drug uptake across a pulmonary epithelial cell line (Calu-3), cultured under air interface conditions. The particle size distribution of DSCG and DSCG/PVA were similar, of spherical geometry, amorphous and suitable for inhalation purposes. Aerosolisation studies using a modified twin-stage impinger showed the DSCG/PVA to have greater aerosol performance than that of DSCG alone. Aerosol particles of DSCG and DSCG/PVA were deposited onto the surface of the Calu-3 air interface epithelium monolayer and the drug uptake from apical to basal directions measured over time. Drug uptake was measured across a range of doses to allow comparison of equivalent drug and powder mass deposition. Analysis of the data indicated that the percentage cumulative drug uptake was independent of the mass of powder deposited, but dependent on the formulation. Specifically, with the formulation containing DSCG, the diffusion rate was observed to change with respect to time (indicative of a concentration-dependent diffusion process), whilst DSCG/PVA showed a time-independent drug uptake (suggesting a zero-order depot release).

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“…To 108 the best of our knowledge, a single report has been published using primary human alveolar 109 epithelial cells to study ion and water transport (Bove et al, 2010). Therefore, respiratory 110 epithelial cell lines are used extensively as representative models because the experimental 111 conditions are more reproducible and easier to control (Mathias, Yamashita & Lee, 1996). 112…”

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confidence: 99%

“…Several cell lines deriving from bronchial epithelial cells, such as Calu-3, BEAS-2B and 113 16HBE14o -, have been shown to be suitable models for studies of airway epithelium 114 (Mathias, Yamashita & Lee, 1996). However, only H441 and A549 cell lines are derived 115 from alveolar epithelial cells and have been characterised to present alveolar epithelial 116 phenotype (Rehan et al, 2002;Lieber et al, 1976).…”

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A cell culture model for alveolar epithelial transport

Hui

Suresh

2014

Preprint

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10Background 11 The thickness and composition of the surface liquid lining the human lungs are maintained by 12 a balance between epithelial secretion and absorption of ions and water. 31 28 Ω• cm 2 ), potential difference (4.8± 0.1 mV) and strong expression of α 1 -Na + -K + -ATPase 32 and tight junction protein ZO-1 consistent with the formation of a polarised epithelium. These 33 cultures also expressed the chloride channel CFTR and all four subunits of the sodium 34 channel ENaC. Cells cultured under medium-medium conditions had a 4-fold higher 35 electrical resistance (1009 ± 15 Ω• cm 2 ), but similar level of potential difference (4.9 ± 0.2 36 mV) and weaker expression of α 1 -Na + -K + -ATPase and ZO-1. The A549 cell line developed 37 low levels of electrical resistance and potential difference and did not express ZO-

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Respiratory epithelial cell culture models for evaluation of ion and drug transport

Cited by 59 publications

References 215 publications

Nanomedicine in pulmonary delivery

Nanomedicine in pulmonary delivery

Modification of Disodium Cromoglycate Passage Across Lung Epithelium In Vitro Via Incorporation into Polymeric Microparticles

A cell culture model for alveolar epithelial transport

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