Respiratory gas transport, haemocyanin function and acid–base balance in Jasus edwardsii during emersion and chilling: simulation studies of commercial shipping methods

Morris, Stephen; Oliver, Susan

doi:10.1016/s1095-6433(99)00004-5

Cited by 26 publications

(12 citation statements)

References 49 publications

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“…We upgraded a gas diffusion chamber (Niesel and Thews, 1961;Sick and Gersonde, 1969;Sick and Gersonde, 1972;Bridges et al, 1984;Morris and Oliver, 1999;Weber et al, 2010) with an integrated fibre-optic micro-pH optode and a miniature broad-range fibre-optic spectrophotometer. The experimental setup offers high flexibility to produce accurate OECs and pH recordings from only minute volumes of sample.…”

Section: Methods and Techniquesmentioning

confidence: 99%

“…The original principle, characterized by full pigment deoxygenation with nitrogen gas followed by the time-dependent diffusion of oxygenated gas into the chamber (Niesel and Thews, 1961;Sick and Gersonde, 1969), was essentially abolished in subsequent studies that continuously perfused the chamber with defined gas mixtures (e.g. Bridges et al, 1984;Morris and Oliver, 1999;Weber et al, 2010). We adopted this amendment and further modified the diffusion chamber as follows.…”

Section: Experimental Setup and Modificationsmentioning

confidence: 99%

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Simultaneous high-resolution pH and spectrophotometric recordings of oxygen binding in native blood microvolumes

Oellermann

Pörtner

Mark

2014

Journal of Experimental Biology

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Oxygen equilibrium curves have been widely used to understand oxygen transport in numerous organisms. A major challenge has been to monitor oxygen binding characteristics and concomitant pH changes as they occur in vivo, in limited sample volumes. Here we report a technique allowing highly resolved and simultaneous monitoring of pH and blood pigment saturation in minute blood volumes. We equipped a gas diffusion chamber with a broad-range fibre-optic spectrophotometer and a micro-pH optode and recorded changes of pigment oxygenation along oxygen partial pressure (P O2 ) and pH gradients to test the setup. Oxygen binding parameters derived from measurements in only 15 μl of haemolymph from the cephalopod Octopus vulgaris showed low instrumental error (0.93%) and good agreement with published data. Broad-range spectra, each resolving 2048 data points, provided detailed insight into the complex absorbance characteristics of diverse blood types. After consideration of photobleaching and intrinsic fluorescence, pH optodes yielded accurate recordings and resolved a sigmoidal shift of 0.03 pH units in response to changing P O2 from 0 to 21 kPa. Highly resolved continuous recordings along pH gradients conformed to stepwise measurements at low rates of pH changes. In this study we showed that a diffusion chamber upgraded with a broad-range spectrophotometer and an optical pH sensor accurately characterizes oxygen binding with minimal sample consumption and manipulation. We conclude that the modified diffusion chamber is highly suitable for experimental biologists who demand high flexibility, detailed insight into oxygen binding as well as experimental and biological accuracy combined in a single setup.

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Section: Methods and Techniquesmentioning

confidence: 99%

Section: Experimental Setup and Modificationsmentioning

confidence: 99%

Simultaneous high-resolution pH and spectrophotometric recordings of oxygen binding in native blood microvolumes

Oellermann

Pörtner

Mark

2014

Journal of Experimental Biology

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“…The effects of air-exposure on the physiology of crustaceans have been thoroughly researched, particularly in the field of physiological homeostasis, exploring the pathways of haemolymph chemical balances (Gäde, 1984;Vermeer, 1987;Paterson et al, 1997;Taylor and Waldron, 1997;Morris and Oliver, 1999;Harris and Andrews, 2005;Ridgway et al, 2006). In our study, pH and the end product of anaerobic metabolism, l-lactate, were assessed and may offer an explanation for the delayed and diminished behavioural responses seen after exposure to air.…”

Section: Haemolymph Chemistrymentioning

confidence: 99%

“…Morris and Oliver (1999) reported a similar decrease in pH values for J. edwardsii from 7.6 for unexposed lobsters, to 7.1 after 5 h of emersion at 18 • C, Vermeer (1987) recorded a decrease in pH from 7.91 to 7.40 after 2 h of air-exposure at 22-30 • C, and Ridgway et al (2006) documented declines from 7.92 to 6.56 when Nephrops norvegicus was exposed to air for 4-12 h at 15-25 • C. Harris and Andrews (2005) noted a decrease from 7.63 to 7.24 after 2 h of on-deck emersion. A decrease in haemolymph pH can reduce the affinity of haemocyanin for oxygen (Vermeer, 1987;Morris and Oliver, 1999) and may interfere with enzymatic reactions, osmoregulation and cell membrane stability (Prosser, 1973). Metabolic acidosis in conjunction with hypoxia would result in exhaustion and explain the immobility and delayed behavioural responses of lobsters exposed to air.…”

Section: Haemolymph Chemistrymentioning

confidence: 99%

“…Metabolic acidosis in conjunction with hypoxia would result in exhaustion and explain the immobility and delayed behavioural responses of lobsters exposed to air. Decreased gas-exchange surface area, due to the collapse of gills in the absence of water, must inhibit the lobsters' ability to expel carbon dioxide (Taylor and Waldron, 1997;Morris and Oliver, 1999). The excess carbon dioxide binds to water to form carbonic acid, which could contribute to the decrease in pH.…”

Section: Haemolymph Chemistrymentioning

confidence: 99%

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Effects of exposure to air on the escape behaviour and haemolymph chemistry of the South African Cape lobster, Jasus lalandii

Haupt

Brouwer²,

Branch

et al. 2006

Fisheries Research

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Impacts of sub‐optimal water quality on the haemolymph biochemistry, immune status and condition of post‐harvest Southern Rock Lobsters (Jasus edwardsii)

Day

Fitzgibbon

Gardner

2022

Aquaculture Fish & Fisheries

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Palinurid rock (or spiny) lobsters constitute valuable seafood fisheries around the world. Post‐harvest mortality can have a substantial economic impact on the value of these fisheries and damage the brand identity of an otherwise fungible commodity. In this study, sudden, large‐scale mortalities in the Australian Rock Lobster industry were investigated through comprehensive haemolymph biochemistry panels, measurements of immune status and nutritional condition. A cohort of lobsters (n = 25) was sampled over a 4‐week period at a commercial processing facility, with the final sample occurring during a mortality event. A comparison of 22 biochemical parameters, including electrolytes, minerals, ions, metabolites and enzymes, showed a notable disruption in osmoregulation, protein content and enzymes during weeks 3 and 4 of the study. Haemocyte counts showed a decline in circulating haemocytes by the time of the mortality event along with a shift in the proportion of hyalinocytes to granulocytes favouring the former. The condition index was variable over the sampling schedule, possibly linked to observed incidences of cannibalism, with no evidence of an overall low condition that would explain mortalities. The lobsters sampled in this study did not show any consistent or progressive changes in biochemistry that may indicate a pathogen or disease as a causative agent. Rather, analysis of the water quality within the facility suggested that mortality was due to sub‐optimal water quality characterized by a combination of two factors, a substantial decrease in the pH and potential nitrogen toxicity, that caused a physiological response comprised of an ion imbalance, altered enzyme activity and, ultimately, mortality. These results offer a comprehensive characterization of the impact of unexplained morbidity on the biochemistry and physiology of lobsters, illustrate the harm of sub‐optimal aquaculture practices and highlight the need for a better understanding of aquaculture practices and their effects on economically important seafood industries.

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Respiratory gas transport, haemocyanin function and acid–base balance in Jasus edwardsii during emersion and chilling: simulation studies of commercial shipping methods

Cited by 26 publications

References 49 publications

Simultaneous high-resolution pH and spectrophotometric recordings of oxygen binding in native blood microvolumes

Simultaneous high-resolution pH and spectrophotometric recordings of oxygen binding in native blood microvolumes

Effects of exposure to air on the escape behaviour and haemolymph chemistry of the South African Cape lobster, Jasus lalandii

Impacts of sub‐optimal water quality on the haemolymph biochemistry, immune status and condition of post‐harvest Southern Rock Lobsters (Jasus edwardsii)

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