ABSTRACT. Respiratory training of premature infants was performed to determine whether improved respiratory muscle strength and/or endurance would result. Twentytwo premature infants were randomized into control and training groups for 2 wk, using inspiratory flow-resistive loads for training (75 cm H 2 0 . L-' . s in wk 1 and 90 cm H20. L-' . s in wk 2). Respiratory endurance was assessed by the time interval required for the development of a 5-torr rise in transcutaneous C02 tension during the hypoventilation induced by loaded breathing, using a moderately severe resistive load (250 cm H 2 0 . L-' . s at 1 L. mine').Respiratory strength was assessed by the maximum negative airway pressure generated during occluded breaths, a pressure-time integral, and an effort index. Results revealed that respiratory muscle endurance, which was not initially different between control and trained groups, increased significantly after 2 wk in the trained group by 137% (median value, p < 0.05), whereas it remained unchanged in the control group (-24%). The trained group of infants also showed a significant decrease in baseline breathing frequency between the initial and final measurements taken 2 wk apart when compared with controls ( p < 0.05) and a lesser increase in inspiratory time with loading in the final measurement as compared with the initial value ( p < 0.05). There was no significant difference between the control and trained groups in initial or subsequent measures of respiratory muscle strength. Inspiratory flow-resistive load training appears to improve the respiratory endurance of premature infants in whom respiratory muscle fatigue has been described to play a role in the development of respiratory failure. (Pediatr Res 31: 613-618,1992) Abbreviations VT tidal volume VE, minute ventilation EI, effort index ET, endurance time IRL, inspiratory flow-resistive load MNAP, maximum negative airway pressure PTI, pressure-time integral TcPco2, transcutaneous C02 tension Ti, inspiratory time