Response and function of cutaneous mucosal and serum antibodies in barramundi (Lates calcarifer) acclimated in seawater and freshwater

Delamare-Deboutteville, J.; Wood, Dan; Barnes, Andrew C.

doi:10.1016/j.fsi.2005.10.005

Cited by 38 publications

(30 citation statements)

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“…One work carried out on Asian sea bass or barramundi (Lates calcarifer) demonstrated that in this species the cutaneous mucosal antibody response was significantly higher in salt water than in fresh water, and both serum and cutaneous mucosal antibodies were capable of binding antigen at salinities in line with seawater existence. The results demonstrate that this adaptive response could be of great importance to euryhaline fish species that are able to exist and move between vastly diverse physiological environments [254].…”

Section: Fish Skin Mucosal Immunologymentioning

confidence: 87%

“…This is particularly important in fishes, which are poikilotherms. In fact, the ASCs found in the skin and gills are directly exposed to these extreme conditions, and their function is therefore more likely to be affected [254]. Whilst there have been some studies on immune response in species of interest in aquaculture, very few have considered the effects of the diversity of environmental factors into the fish mucosal immunity.…”

Section: Fish Skin Mucosal Immunologymentioning

confidence: 99%

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An Overview of the Immunological Defenses in Fish Skin

2012

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The vertebrate immune system is comprised of numerous distinct and interdependent components. Every component has its own inherent protective value, and the final combination of them is likely to be related to an animal's immunological history and evolutionary development. Vertebrate immune system consists of both systemic and mucosal immune compartments, but it is the mucosal immune system which protects the body from the first encounter of pathogens. According to anatomical location, the mucosa-associated lymphoid tissue, in teleost fish is subdivided into gut-, skin-, and gill-associated lymphoid tissue and most available studies focus on gut. The purpose of this paper is to summarise the current knowledge of the immunological defences present in skin mucosa as a very important part of the fish immune system, serving as an anatomical and physiological barrier against external hazards. Interest in defence mechanism of fish arises from a need to develop health management tools to support a growing finfish aquaculture industry, while at the same time addressing questions concerning origins and evolution of immunity in vertebrates. Increased knowledge of fish mucosal immune system will facilitate the development of novel vaccination strategies in fish.

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Section: Fish Skin Mucosal Immunologymentioning

confidence: 87%

Section: Fish Skin Mucosal Immunologymentioning

confidence: 99%

An Overview of the Immunological Defenses in Fish Skin

2012

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“…Delamare-Deboutteville, Wood [26] used immunisation (i.e. by injection and immersion in formalin killed …”

Section: Discussionmentioning

confidence: 99%

Influence of Maternal and Larval Immunisation against <i>Lactococcus garviae</i> Infection in Rainbow Trout <i>Oncorhynchus mykiss</i> (Walaum) Lysozyme Activity and IgM Level

Akbary¹,

Mirvaghefi²,

Akhlaghi³

et al. 2015

OJAS

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How to cite this paper: Akbary, P., Mirvaghefi, A.R., Akhlaghi, M. and Fereidouni, M.S. (2015) AbstractThis study evaluated efficacy of maternal and larval immunisation against Lactococcus garviae infection and on the lysozyme and immunoglobulin (IgM) levels in rainbow trout Oncorhynchus mykiss (Walaum). Forty-eight-day-old larvae (mean weight 96 mg) originating from injected weekly with letrozole and immunised, only immunised and non-immunised parents were experimentally infected with the L. garvieae, and the mortality rate was recorded daily. Larvae were vaccinated by immersion at 58 days post hatch with live L. garvieae (10 9 cells/mL) for 15 min. Every third day post larvae vaccination, two larvae from each group were collected for analysis lysozyme (by a method based on the ability of lysozyme to lyse the bacterium Micrococcus lysodeikticus) and IgM (by enzyme-linked immunosorbent assay (ELISA)) parameters. Vaccinated and control larvae were tested for protection against L. garvieae 30 days post larvae immunization when the larvae were 88 days old. Larvae were challenged by bath exposure with live L. garvieae (10 9 cells/mL) for 2 min and monitored for mortality for at least 10 days following challenge. The challenge experiment with L. garvieae showed a significant reduction in larvae from immunised (54.44% ± 0.64%) and injected weekly with letrozole and immunised fish (52.96% ± 0.97%) compared to larvae from control fish (62.96% ± 2.22%). Vaccinated larvae originated from injected weekly with letrozole and immunised parents showed significantly higher lysozyme activity compared to other fish groups. Vaccinated larvae showed significantly less mortality compared to controls. The rela-* Corresponding author.

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“…injection, non-specific gut and skin-associated mucosal immunity may play an important role in the protection against the bacteria (Delamare- Deboutteville et al, 2006;Rombout Jan H.W.M et al, 2011;Salinas et al, 2011;Urquhart et al, 2009). Transmission of GBS via water-born immersion or through food exposure most closely mimic natural routes of infections, as they do not compromise the tegument of the fish involved in protection against invading pathogen, which will occur when challenging by i.p.…”

Section: Transmission Of Gbs Via Water-born Exposure Has Been Previoumentioning

confidence: 99%

“…The conditions required for these fish to contain the infection are not clear, but non-specific gut and skin-associated mucosal immunity may play an important role in the protection against bacteria (Delamare-Deboutteville et al, 2006;Rombout et al, 2011;Salinas et al, 2011;Urquhart et al, 2009). …”

Section: Group B Streptococcus Is Likely To Be the Cause Of The Diseamentioning

confidence: 99%

On the origin of Group B Streptococcus from disease outbreaks in wild marine fish in Australia

Deboutteville¹

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Since 2007, 96 wild Queensland groupers, Epinephelus lanceolatus, were found dead from Karumba (Gulf of Carpentaria) in Northern Territory to Brisbane in southern Queensland, Australia. In twelve cases, Streptoccocus agalactiae (Group B Streptococcus, GBS) was isolated in pure culture from eyes and internal organs. GBS has been also isolated from other marine species (Javelin grunter, Giant catfish and Squaretail mullet) and caused an outbreak in three species of wild stingrays (Eastern shovelnose ray, Mangrove whipray and Estuary ray) in a public aquarium also in Queensland. These findings have raised public and industry concern over threats to human health from potential transmission between fish and humans, over environmental impacts on other aquatic animals, and over the potential threat posed to the growing Australian aquaculture industry in which, to date, there have been no reported cases of this disease in spite of the devastation caused to the industry overseas. Therefore, my thesis set out to determine whether GBS isolated from dead wild fish in Australia is indeed virulent in Queensland grouper and causative of mortality, to try to identify from where these infections in wild fish originated, to determine potential risk to human health and to other aquatic animals, and to begin to identify mechanisms by which these Australian isolates may cause fatal disease in Queensland grouper. This information may be employed in future risk assessment of potential human transfer, in mitigating further outbreaks in wild fish through understanding origin of infection, and in future development of preventative measures such as vaccination for farmed fish.In order to confirm that S. agalactiae was the cause of the mortality in wild fish, experimental challenges were conducted by injection, immersion and through the oral route. To better understand why these strains of GBS are pathogenic in fish, we conducted a series of cellular immune assays using juvenile Queensland grouper head-kidney leucocytes in the presence of different piscine and terrestrial isolates. Whole-genome sequencing analysis of the piscine and terrestrial isolates provided information on potential origin and potential for transfer between species, and also informed us on critical virulence factors that could be used during pathogenesis of GBS. Moreover, genome analysis gave intriguing insight into the evolution and adaption of GBS to fish as a host and to life in the aquatic environment. To determine putative origin of GBS in wild fish in Australia, multilocus sequence typing (MLST) and capsular polysaccharide (CPS) were derived from whole-genome sequences and revealed that all piscine isolates from Australia were serotype Ib and fell into ST-261 lineage first identified in 1986 from Tilapia in Israel. Overseas, ST-261 has only been found in fish and poikilotherm animals, and has never been associated with humans. High similarity and identical point mutations in the capsular operon of the piscine isolates suggests a common source of infection...

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Response and function of cutaneous mucosal and serum antibodies in barramundi (Lates calcarifer) acclimated in seawater and freshwater

Cited by 38 publications

References 20 publications

An Overview of the Immunological Defenses in Fish Skin

An Overview of the Immunological Defenses in Fish Skin

Influence of Maternal and Larval Immunisation against <i>Lactococcus garviae</i> Infection in Rainbow Trout <i>Oncorhynchus mykiss</i> (Walaum) Lysozyme Activity and IgM Level

On the origin of Group B Streptococcus from disease outbreaks in wild marine fish in Australia

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Response and function of cutaneous mucosal and serum antibodies in barramundi (Lates calcarifer) acclimated in seawater and freshwater

Cited by 38 publications

References 20 publications

An Overview of the Immunological Defenses in Fish Skin

An Overview of the Immunological Defenses in Fish Skin

Influence of Maternal and Larval Immunisation against &lt;i&gt;Lactococcus garviae&lt;/i&gt; Infection in Rainbow Trout &lt;i&gt;Oncorhynchus mykiss&lt;/i&gt; (Walaum) Lysozyme Activity and IgM Level

On the origin of Group B Streptococcus from disease outbreaks in wild marine fish in Australia

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Influence of Maternal and Larval Immunisation against <i>Lactococcus garviae</i> Infection in Rainbow Trout <i>Oncorhynchus mykiss</i> (Walaum) Lysozyme Activity and IgM Level