Response interactions in grape somatic embryogenic cultures to cold and gibberellic acid treatments to overcome embryo dormancy

Larrouy, Justine; Jaksons, Peter; Bicknell, Ross

doi:10.1007/s11240-016-1154-7

Cited by 7 publications

(4 citation statements)

References 15 publications

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“…It can be said that both GA 3 and cold treatments can increase the embryo conversion rates, but their effects are highly depending on the genotype. This result indicates that both factors affect the same biological process as earlier proposed by several authors [43,49,52,[55][56][57].…”

Section: Embryo Conversionsupporting

confidence: 85%

“…Despite previous reports showing that cold treatment as well as gibberellic acid, can successfully be used to overcome embryo dormancy in grape somatic embryos [43,52,54], in the present research, the positive effect of those factors, highly depended on the genotype.…”

Section: Embryo Conversioncontrasting

confidence: 72%

“…The conversion of somatic embryos into plants is reported as an additional difficulty to establish efficient SE protocols. This is related with the difficulty in producing mature and well-developed somatic embryos able to grow into normal plants [39], or associated with the difficulty to break embryo dormancy in a similar way that occurs in natural dormancy of most seeds [52].…”

Section: Embryo Conversionmentioning

confidence: 99%

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Somatic Embryogenesis in Iberian Grapevine (Vitis vinifera) Cultivars Using Carpels as Initial Explants: Protocol Establishment and Histological Evaluation

Cardoso¹,

Campos²,

Pais³

et al. 2019

JAST-B

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Trincadeira and Aragonez are two important grapevine cultivars in the Iberian Peninsula, used for high quality red wines production. Both cultivars are strongly affected by fungal diseases, with consequent high loses on plant productivity and fruit quality. A successful protocol for plant regeneration via somatic embryogenesis (SE) was established for both cultivars allowing further plant improvement based on gene transfer technology. Several factors were evaluated during the three different phases which characterize an SE plant regeneration protocol. The culture room temperature during the induction phase, a parameter usually accepted as standard by most researchers, proved in these trials to significantly affect the embryo induction rates. Concerning embryo conversion, it was specially affected by the embryo developmental stage, by the intensity and duration of the chilling treatment and by the supplementation of conversion culture medium with activated charcoal. The responses obtained, both for induction and conversion, proved to be highly genotype dependent. Calli structure, as well as embryo integrity, was histologically observed, allowing to characterize embryonic and non-embryonic masses and to identify abnormalities on embryo development.

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Section: Embryo Conversionsupporting

confidence: 85%

Section: Embryo Conversioncontrasting

confidence: 72%

Section: Embryo Conversionmentioning

confidence: 99%

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Somatic Embryogenesis in Iberian Grapevine (Vitis vinifera) Cultivars Using Carpels as Initial Explants: Protocol Establishment and Histological Evaluation

Cardoso¹,

Campos²,

Pais³

et al. 2019

JAST-B

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“…However, one of the major limitations of this regeneration technique regards embryo endodormancy, resulting in low germination rate and plant development ( Gray and Purohit, 1991 ; Larrouy et al., 2017 ). Variable germination rates, often lower than 50%, were reported in somatic embryogenesis studies ( Martinelli et al., 2001b ; Carimi et al., 2005 ).…”

Section: Introductionmentioning

confidence: 99%

Efficient protocol of de novo shoot organogenesis from somatic embryos for grapevine genetic transformation

Capriotti,

Ricci,

Molesini

et al. 2023

Front. Plant Sci.

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Plant genetic transformation is a powerful tool that can facilitate breeding programs for disease tolerance, abiotic stress, fruit production, and quality by preserving the characteristics of fruit tree elite genotypes. However, most grapevine cultivars worldwide are considered recalcitrant, and most available genetic transformation protocols involve regeneration by somatic embryogenesis, which often requires the continuous production of new embryogenic calli. Cotyledons and hypocotyls derived from flower-induced somatic embryos of the Vitis vinifera cultivars Ancellotta and Lambrusco Salamino, in comparison with the model cultivar Thompson Seedless, are here validated for the first time as starting explants for in vitro regeneration and transformation trials. Explants were cultured on two different MS-based culture media, one having a combination of 4.4 µM BAP and 0.49 µM IBA (M1), and the other only supplemented with 13.2 µM BAP (M2). The competence to regenerate adventitious shoots was higher in cotyledons than in hypocotyls on both M1 and M2. M2 medium increased significantly the average number of shoots only in Thompson Seedless somatic embryo-derived explants. This efficient regeneration strategy, that proposes a combination of somatic embryogenesis and organogenesis, has been successfully exploited in genetic engineering experiments. Ancellotta and Lambrusco Salamino cotyledons and hypocotyls produced the highest number of calli expressing eGFP when cultured on M2 medium, while for Thompson Seedless both media tested were highly efficient. The regeneration of independent transgenic lines of Thompson Seedless was observed from cotyledons cultured on both M1 and M2 with a transformation efficiency of 12 and 14%, respectively, and from hypocotyls on M1 and M2 with a transformation efficiency of 6 and 12%, respectively. A single eGFP fluorescent adventitious shoot derived from cotyledons cultured on M2 was obtained for Ancellotta, while Lambrusco Salamino showed no regeneration of transformed shoots. In a second set of experiments, using Thompson Seedless as the model cultivar, we observed that the highest number of transformed shoots was obtained from cotyledons explants, followed by hypocotyls and meristematic bulk slices, confirming the high regeneration/transformation competences of somatic embryo-derived cotyledons. The independent transformed shoots obtained from the cultivars Thompson Seedless and Ancellotta were successfully acclimatized in the greenhouse and showed a true-to-type phenotype. The novel in vitro regeneration and genetic transformation protocols optimized in this study will be useful for the application of new and emerging modern biotechnologies also to other recalcitrant grapevine genotypes.

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Progress and Challenges in the Application of Synthetic Seed Technology for Ex Situ Germplasm Conservation in Grapevine (Vitis spp.)

Carra¹,

Carimi²,

Bettoni

et al. 2019

Synthetic Seeds

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Response interactions in grape somatic embryogenic cultures to cold and gibberellic acid treatments to overcome embryo dormancy

Cited by 7 publications

References 15 publications

Somatic Embryogenesis in Iberian Grapevine (Vitis vinifera) Cultivars Using Carpels as Initial Explants: Protocol Establishment and Histological Evaluation

Somatic Embryogenesis in Iberian Grapevine (Vitis vinifera) Cultivars Using Carpels as Initial Explants: Protocol Establishment and Histological Evaluation

Efficient protocol of de novo shoot organogenesis from somatic embryos for grapevine genetic transformation

Progress and Challenges in the Application of Synthetic Seed Technology for Ex Situ Germplasm Conservation in Grapevine (Vitis spp.)

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