Response of a Mu-class glutathione S-transferase from black tiger shrimp Penaeus monodon to aflatoxin B1 exposure

Abstract

Glutathione S-transferases (GSTs) are a family of multifunctional phase II enzymes that are involved in the detoxification of exogenous and endogenous compounds. In this study, a full-length cDNA of Mu-class GST (PmMuGST) was isolated from the hepatopancreas of Penaeus monodon using rapid amplification of cDNA ends method. The full length cDNA of PmMuGST is 867 bp, contains an open read frame of 660 bp, and encodes a polypeptide of 219 amino acids with a molecular mass of 25.61 kDa and pI of 6.15. Sequence analysis indicated that the predicted protein sequence of PmMuGST was very similar to (86 %) that of Litopenaeus vannamei. A conserved domain of GST_N_Mu_like (PSSM: cd03075) and GST_C_family_superfamily_like (PSSM: cl02776) was indentified in PmMuGST. Real time quantitative RT-PCR analysis indicated that PmMuGST was present in all of the tested tissues. PmMuGST transcripts both in the hepatopancreas and in the muscle were significantly induced after 14 days of treatment with a low dosage of AFB1 (50 μg/kg) exposure and were significantly inhibited after 42 and 56 days of a high dosage of AFB1 (1000, 2500 μg/kg AFB1) exposure. Taken together, the Mu-class GST from P. monodon was inducible and was involved in the response to AFB1 exposure.

Hepatopancreatic necrosis disease (HPND) in Chinese mitten crab Eriocheir sinensis tightly linked to low concentration of two insecticides

“…Histopathology in this work demonstrated that the two insecticides were respectively damaged the hepatopancreas of E. sinensis . The hepatopancreas is an extremely important organ to the crabs, which can secrete a variety of enzymes, participating in the growth, development, moulting, material metabolism, nutrient metabolism and other life activities (Hong, Huang, Yan, Pan & Zhang, 2019; Wang et al, 2016). The hepatopancreas became deformation, enlargement and had many vacuoles after exposure.…”

Identification and expression profile of Delta‐GST in black tiger shrimp (Penaeus monodon) exposed to aflatoxin B1 (AFB1)

“…Upregulation of one or several of the detoxification enzyme genes has been reported to be involved in abamectin, pyrethroid, and meonicotinoid insecticide resistance or detoxification in insects (Stumpf & Nauen, ; Vontas, Small, & Hemingway, ). Increased expression levels of PmMGST3 from Pinctada martensii and PmMuGST from P. monodon have a specialized role in cadmium and AFB1 detoxification (J. H. Chen, Xiao, Deng, Du, & Yu, ; Wang et al, ). Enhanced expression of MdGST‐6A from Musca domestica and PxGST3 from Plutella xylostella was associated with organophosphate detoxification (H. S. Huang et al, ; Wei, Clark, & Syvanen, ).…”

“…The glutathione conjugates of AFB1‐epoxides are water‐soluble aflatoxin mercapturic acids (AFB1‐NAC), making them more soluble and easier to be excreted from the cells (Egner, Groopman, Wang, Kensler, & Friesen, ). Exposure to AFB1 causes poor growth, immunosuppression, behavioral abnormalities, low apparent digestibility, histological changes, reproduction impairment as well as traces of AFB1 accumulation in the liver and other tissues in gibel carp Carassius auratus gibelio , white shrimp Litopenaeus vannamei , tilapia Oreochromis niloticus and P. monodon (Y. Huang et al, ; Hussain, Mateen, & Gatlin, ; Wang et al, ; Zeng et al, ). Recently, more researchers are focused on the oxidative damage induced by a diet contaminated with AFB1 in aquatic animals including silver catfish Rhamdia quelen and grass carp Ctenopharyngodon idella (Souza et al, ; Zeng et al, ).…”

“…The N‐terminal G‐site binds to glutathione and is highly conserved, whereas the C‐terminal H‐site recognizes hydrophobic substrates (Richardson, Gold‐Bouchot, & Schlenk, ). Some isoforms of GSTs have been cloned and characterized from aquatic invertebrates including L. vannamei (Contreras‐Vergara, Harris‐Valle, Sotelo‐Mundo, & Yepiz‐Plascencia, ), Chinese shrimp Fenneropenaeus chinensis (Ren, Sun, Zhao, & Wang, ), freshwater prawn Macrobrachium rosenbergii (Arockiaraj et al, ), P. monodon (Wang et al, ), African river prawns Macrobrachium vollenhovenii (Adewale & Afolayan, ), clam Atactodea striata (Calmes et al, ), and pacific oyster Crassostrea gigas (Boutet, Tanguy, & Moraga, ).…”

“…characterized from aquatic invertebrates including L. vannamei (Contreras-Vergara, Harris-Valle, Sotelo-Mundo, & Yepiz-Plascencia, 2004), Chinese shrimp Fenneropenaeus chinensis (Ren, Sun, Zhao, & Wang, 2009), freshwater prawn Macrobrachium rosenbergii (Arockiaraj et al, 2014), P. monodon (Wang et al, 2016), African river prawns Macrobrachium vollenhovenii (Adewale & Afolayan, 2005), clam Atactodea striata (Calmes et al, 2015), and pacific oyster Crassostrea gigas (Boutet, Tanguy, & Moraga, 2004).…”