Response of antioxidant enzymes to excess copper in tomato (Lycopersicon esculentum, Mill.)

Mazhoudi, Salma; Chaoui, Abdelilah; Ghorbal, Mohamed Habib; Ferjani, Ezzedine El

doi:10.1016/s0168-9452(97)00116-7

Cited by 246 publications

(129 citation statements)

References 28 publications

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“…In the roots, however, the enzyme was inhibited in both species, likely due to the higher Cd concentrations accumulated there. Cadmium at high concentrations may have a direct effect on protein biosynthesis (Mazhoudi et al, 1997). It may also induce the accumulation of hydroperoxides or other by-products of ROS action, leading to GST inhibition (Nagalakshmi and Prasad, 2001), as was observed here in the roots of both species.…”

Section: Resultssupporting

confidence: 73%

Cadmium-induced oxidative stress and antioxidative enzyme response in water hyacinth and salvinia

Vestena¹,

Cambraia²,

Ribeiro³

et al. 2011

Braz. J. Plant Physiol.

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The reactive oxygen species generation, lipid peroxidation and antioxidative enzyme response of water hyacinth and salvinia to Cd were evaluated. Cadmium was absorbed/accumulated mainly in the roots, but significant amounts also translocated to the leaves. No Cd effect on dry weight was detected, although toxicity symptoms were visible. Superoxide and H 2 O 2 concentrations increased, in addition to lipid peroxidation in both species, especially in the leaves of salvinia. In general, antioxidative enzyme activities were reduced in both species following Cd treatment, especially in salvinia. Glutathione peroxidase (GPX, EC 1.11.1.9) activity decreased in water hyacinth but increased in salvinia. Glutathione S-transferase (GST, EC 2.5.1.18) activity increased in the leaves but decreased in the roots of both species. So, Cd induced ROS generation/accumulation, but the antioxidative enzymes were not able to combat the Cd-induced oxidative injury in these two species. Nevertheless, water hyacinth consistently showed a higher tolerance to Cd than salvinia.

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Section: Resultssupporting

confidence: 73%

Cadmium-induced oxidative stress and antioxidative enzyme response in water hyacinth and salvinia

Vestena¹,

Cambraia²,

Ribeiro³

et al. 2011

Braz. J. Plant Physiol.

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“…GPX was measured by the method of Mazhoudi et al (1997). The reaction mixture contained 50mM phosphate buffer, 0.2mM guaiacol, 10mM H 2 O 2 and distilled water in the total volume of 3.0 ml.…”

Section: Methodsmentioning

confidence: 99%

The effect of brassinosteroids on radish (Raphanus sativus L.) seedlings growing under cadmium stress

Anuradha¹,

Rao²

2007

Plant Soil Environ.

103

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The effect of 24-epibrassinolide and 28-homobrassinolide on seed germination and seedling growth of radish (Raphanus sativus L.) was studied under cadmium toxicity. The impact of brassinosteroids (BRs) on free proline levels and the activity of antioxidant enzymes catalase (CAT; EC 1.11.1.6), peroxidase (POD; EC 1.11.1.7), superoxide dismutase (SOD; EC 1.15.1.1), ascorbic peroxidase (APOX; EC 1.11.1.11) and guaiacol peroxidase (GPX; EC 1.11.1.7) in radish seedlings under Cd toxicity was evaluated. The effect of BRs on the activity of ascorbic acid oxidase (AAO; EC 1.10.3.3) and lipid peroxidation in radish seedlings challenged with Cd stress was also investigated. BRs supplementation alleviated the toxic effect of the heavy metal and increased the percentage of seed germination and seedling growth. Out of the two substances, HBL was found to be more effective than EBL in stress alleviation. HBL (3µM) alleviated the toxic effect of the heavy metal and increased the percentage of seed germination by 57% over Cd and 20% over unstressed control. Similarly supplementation of HBL (3µM) caused an increase of 156%, 78% and 91% in length, fresh weight and dry weight of seedling, respectively, over Cd treatment alone. The amelioration of seedling growth by BRs under metal toxicity was associated with enhanced levels of free proline. The activities of antioxidant enzymes CAT, SOD, APOX and GPX were increased in the seedlings from treatments with Cd along with BRs. Brassinosteroid treatment reduced the activity of POD and AAO in heavy metal stressed seedlings. Lipid peroxidation induced by Cd was found reduced with the supplementation of BRs. The results obtained in the study clearly indicated the ameliorative influence of brassinosteroids on the inhibitory effect of Cd toxicity.

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“…Radicais livres são formados sob condições de estresse oxidativo bem como pelas reações normais da cadeia de transporte de elétrons, mas que são altamente reguladas Mazhoudi et al, 1997;Greggains et al, 2000), todavia esta regulação pode ser perdida se o estresse for mais severo aumentando consideravelmente a produção de radicais livres que podem levar a uma cascata de evento potentials, induced by mannitol (0; -0.3; -0.6; -0.9 and -1.2MPa) and one with different temperatures (20, 25, 30 e 35ºC).The salicylic acid treatment of 0,025 mM was positive for germination and the germination speed index of Calendula officinalis Marigold seeds under ideal conditions and water stress at the temperature of 35 C.…”

Section: Introductionunclassified

Ácido salicílico em sementes de calêndula (Calendula officinalis L.) sob diferentes estresses

Carvalho

Neto

Custódio

2007

Rev. bras. sementes

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As plantas sofrem agressões por agentes bióticos e abióticos e apesar de não apresentarem defesas através de movimentos ágeis, podem ocorrer adaptações e profundas alterações no metabolismo celular, entre elas a síntese de proteínas de defesas, ativada através de mecanismos complexos. A aplicação exógena ou o estímulo à síntese endógena de ácidos orgânicos como o ácido salicílico, pode agir como indutor de proteínas de tolerância aos diferentes estresses, bem como para elevar a atividade de enzimas de desintoxicação celular, especialmente às envolvidas na degradação de radicais ativos oxigenados. O objetivo deste trabalho foi estudar o efeito do ácido salicílico sobre a germinação e o vigor de sementes de calêndula (Calendula officinalis L.) em condições ideais e sob estresse térmico e hídrico. As sementes foram colocadas para germinar em papel embebido em soluções crescentes de ácido salicílico (zero, 0,0125, 0,025, 0,05, 0,1 e 0,2mM); medindo-se as variáveis: percentagem de germinação; índice de velocidade de germinação e primeira contagem da germinação. Ficou constatado através do teste de Tukey que apenas a germinação foi significativa, sendo que as melhores dosagens de sementes germinadas ficou entre 0,025 e 0,05mM de ácido salicílico. Três outros experimentos foram feitos, um com água acidulada aos pH respectivos às concentrações de ácido salicílico (6,0; 4,8; 4,2; 3,6 e 3,2), um com diferentes potenciais hídricos induzidos por manitol (0; -0,3; -0,6; -0,9 e -1,2MPa), e outro com temperaturas (20, 25, 30 e 35ºC). O ácido salicílico na dose de 0,025mM interferiu positivamente na percentagem de germinação e no índice de velocidade de germinação de sementes da calêndula em condições ideais e sob efeito de estresse hídrico e térmico a 35º.

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Response of antioxidant enzymes to excess copper in tomato (Lycopersicon esculentum, Mill.)

Cited by 246 publications

References 28 publications

Cadmium-induced oxidative stress and antioxidative enzyme response in water hyacinth and salvinia

Cadmium-induced oxidative stress and antioxidative enzyme response in water hyacinth and salvinia

The effect of brassinosteroids on radish (Raphanus sativus L.) seedlings growing under cadmium stress

Ácido salicílico em sementes de calêndula (Calendula officinalis L.) sob diferentes estresses

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