Response of Different Explants for  Callus Induction in Cucumber

Sultana, Hasina; Nahar, Lutfun; Hossain, MM; Ghosh, Totan Kumar; Biswas, Md. Sanaullah

doi:10.24018/ejbio.2021.2.5.278

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…Sultana et al [29] studied that leaf disc explant of cucumber recorded maximum callus induction percentage and dry matter accumulation as well as minimum days to callus initiation with application of 2 mg L -1 2,4 -D in culture medium.…”

Section: Cucumbermentioning

confidence: 99%

Tissue Culture: Aeon of Micro Propagation in Vegetable Crops

Chaudhari

2023

IJECC

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Tissue culture in vegetable crops is a technique that has revolutionized the way we produce and propagate plants. It involves the growth of plant cells, tissues or organs in an artificial nutrient medium under sterile conditions. This method offers numerous advantages, including the production of disease-free plants, rapid multiplication and the ability to grow plants year-round. One of the key benefits of tissue culture in vegetable crops is the production of disease-free plants. By starting with a small piece of healthy tissue such as a leaf or stem, it is possible to grow an entire plant that is free from any pathogens. This is particularly important in vegetable crops, as diseases can significantly reduce yields and quality. By using tissue culture, farmers can ensure that the plants they grow are healthy and resistant to common diseases. Another advantage of tissue culture is the rapid multiplication of plants. Through a process called micropropagation, a single piece of tissue can be used to produce hundreds or even thousands of identical plants within a short period. This is particularly useful for vegetable crops that have a high demand or are difficult to propagate through traditional methods. By using tissue culture, farmers can quickly and efficiently produce large quantities of plants to meet market demands. Furthermore, tissue culture allows for year-round plant production. Unlike traditional methods that are limited by seasonal variations, tissue culture can be done in controlled environments such as laboratories or greenhouses. This means that farmers can grow vegetable crops regardless of the weather conditions outside. This is particularly advantageous for regions with harsh climates or limited growing seasons.

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Section: Cucumbermentioning

confidence: 99%

Tissue Culture: Aeon of Micro Propagation in Vegetable Crops

Chaudhari

2023

IJECC

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“…In vitro regeneration of cucumber can be achieved by using different cell and tissue culture techniques. Regeneration via organogenesis and somatic embryogenesis has been described in this species, and commonly used explants include cotyledons [26][27][28][29], cotyledonary nodes [26,[30][31][32], hypocotyls [27,[33][34][35], shoot tips [36][37][38][39], and leaves [27,37,40,41]. Regeneration of cucumber has also been reported from protoplasts [42][43][44] and suspension cultures [45][46][47].…”

Section: Introductionmentioning

confidence: 99%

Influence of High Concentrations of Copper Sulfate on In Vitro Adventitious Organogenesis of Cucumis sativus L.

Miguel

2023

IJPB

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Copper (Cu) is an essential plant micronutrient. This report is the first to assess the effects of high copper sulfate (CuSO4) levels on in vitro callus and shoot formation of cucumber. Four-day-old cotyledon explants from the inbred line Wisconsin 2843 and the commercial cultivars Marketer and Negrito were used. Murashige and Skoog (MS)-derived callus and shoot induction medium containing 0.5 mg L−1 indole-3-acetic acid (IAA) and 2.5 mg L−1 6-benzylaminopurine (BAP) was supplemented with CuSO4 (0.2–5 mg L−1). The response on callus-derived shoots showed that the optimal concentration of CuSO4 was 8- to 200-fold greater than in standard MS medium. Shoot frequency (SF) and shoot number (SN) were assessed, and Marketer > Negrito > Wisconsin 2843, where 1, 0.2 and 5 mg L−1 CuSO4 produced the highest results, respectively. SF and SN increased 6- and 10-fold in Wisconsin 2843 and twice in the other cultivars. All explants formed calluses, and in two of the three cultivars, callus extension was significantly affected by CuSO4 application. SN showed a strong relationship with CuSO4 levels and no association with callus extension. The results show that specific CuSO4 concentrations higher than in standard MS medium increase adventitious cucumber shoot organogenesis.

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Response of Different Explants for Callus Induction in Cucumber

Cited by 2 publications

References 17 publications

Tissue Culture: Aeon of Micro Propagation in Vegetable Crops

Tissue Culture: Aeon of Micro Propagation in Vegetable Crops

Influence of High Concentrations of Copper Sulfate on In Vitro Adventitious Organogenesis of Cucumis sativus L.

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