Response of Gelatin Modified Electrode towards Sensing of Different Metabolites

Abstract

In this study, a very thin film of biocompatible gelatin B (GB) fabricated onto indium tin oxide (ITO)-coated glass substrate for electrochemical catalytic activity towards different metabolites has been investigated. The optical and electrochemical properties of bare GB/ITO electrode and with different metabolites were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and electrochemical techniques. The optical properties clearly indicate the structural and surface morphological changes on electrode surface. FTIR spectra showed displacement of the IR peaks towards smaller wave numbers, indicating possible existence of hydrogen bonding between the GB and metabolites. The catalytic behaviour of GB/ITO electrode towards ascorbic acid (AA), citric acid (CA), oxalic acid (OA), glucose (Glu), sucrose (Suc), lactose (Lac) and fructose (Fru) has been investigated by cyclic voltammetry (CV). The electrochemical response studies of GB/ITO electrode have been monitored with different metabolites in the range of 10-500 mg/dl. The sensitivity of GB/ITO electrode for AA and OA was found as 0.156 and 0.108 μA/(mg/dl cm(-2)) respectively. The results indicate that the GB/ITO electrode has higher specificity towards the AA and OA. The attractive properties of GB/ITO electrode provide the potential applications in the simultaneous detection of AA and OA. The excellent electrocatalytic behaviour of GB/ITO electrode may be useful towards the construction of electrochemical biosensors.

Biocompatible laponite ionogels based non-enzymatic oxalic acid sensor

“…The optimization studies were necessary to explicitly determine the dependence of various experimental parameters like concentration, scan rate, pH, pulse amplitude etc. to establish the best experimental working condition [12,13,[30][31][32].…”

“…Clays are used in biosensors as immobilization matrices due to their large specific surface area, good absorbance ability and high cationic exchange capacity [3][4][5][6]. Due to the property of laponite to form non-ergodic soft solids in the aqueous dispersions, they have been used to cast films onto electrodes for the immobilization of proteins [7][8][9][10][11] Rawat et al have studied the aspect ratio and concentration dependent cytotoxicity and antimicrobial activity of laponite and Montmorillonite nanoclay [12,13].…”

Sandwich-Type Enzymatic Fuel Cell Based on a New Electro-Conductive Material - Ion Jelly

“…Alkyl imidazolium cation‐based ILs are commonly studied; 1‐ethyl‐3‐methylimidazolium ethylsulfate ([emim][EtSO4]), with its high dielectric constant and wide electrochemical window, is particularly suitable for electrochemical applications . Similar to studies employing gelatin‐based sensors for metabolites, IJ is an advantageous matrix as the gelatin provides a suitable environment for biomolecule immobilization, and the IL confers high conductivity. Immobilization of redox proteins onto this matrix can potentially overcome the issue of building an efficient electron transfer pathway between the active site of the enzyme and the electrode.…”

Gelatin Nanoparticles as a Delivery System for Proteins

“…Gelatin is a polyampholyte consisting of both cationic and anionic groups along with a hydrophilic group. It is known that the mechanical properties such as swelling behavior and thermal properties of gelatin NPs depend significantly on the degree of cross linking between cationic and anionic groups [6][7]. Several methods have been used to synthesize GNPs, including desolvation, coacervation, and water-in-oil (w/o) emulsion, solvent worse or precipitation [8].…”