A new ecological substrate is proposed to achieve a desired electric conduction and heating to protect the slope plants from freeze injury. Expanded polystyrene (EPS), cement, carbon fiber, graphite, and raw soil are the main components of the ecological substrate. The electrical conductivity, heating efficiency, thermo-sensitivity, and heat preservation of the substrate are experimentally investigated. The result shows that the addition of carbon fiber could significantly decrease resistivity of substrate, but the effect of fiber content exceeding 3% on the resistivity of substrate becomes insignificant. Conductive fine materials (i.e., carbon fiber and graphite powder) covering the surface of EPS would result in a significant reduction of the global resistivity of non-dry substrate, but it could only slightly affect the counterpart of the completely dry substrates. The substrate could hardly be formed when the EPS content exceeds 4%. As EPS content increases, the contact surface decreases and the resistivity of the substrate increases. The peak temperature at 30 min of substrate without root is higher than that of substrate with plant roots. Nevertheless, the temperature alteration ratio below 40 • C of substrate with plant root is nearly the same as its counterpart in the substrate without roots. The resistance of the substrate with plant roots increases with the temperature. The resistance of rootless substrate decreases by the heat action of the loosely bound water. EPS particles improve the heat preservation performance of substrate, but the heat preservation performance of substrate degrades with the growth of plants.roots grow, it would provide anchoring into bedrock or stable soil layers, which would be further favorable for the slope stability [12][13][14].Due to the high production efficiency, soil-spraying technology, which was first developed by Japanese scholars [15], has been widely used in vegetation engineering for slope restoration. Nevertheless, it was reported that it is difficult to facilitate vegetation on the steep slopes [16]. To address this issue, various researchers [17] attempted to spray the ecological substrates on the slope surface for the slope restoration. The ingredients of the ecological substrate are usually composed of fibers, loam, cement, and a pH-adjusting agent, etc. literature [18] indicated that the ecological substrate can greatly promote the plants' growth, thereby effectively restoring the slope. However, it has been reported that the young plants for slope protection, such as cynodondactylon and zoysiagrass, would stop growing and be fatal to the cold weather, especially when it encounters continuous snow in the winter [19,20]. However, to date, little attention has been paid to the functionality of ecological substrate in protecting the plant growth in extremely cold weather, in contrast to the studies on the vegetative properties of ecological substrate [21,22].Therefore, the current work aims to propose a new type of ecological substrate with the capability of pro...