Response of nitrogen fixation in relation to nodule carbohydrate metabolism in Medicago ciliaris lines subjected to salt stress

Salah, Imen Ben; Albacete, Alfonso; Martínez‐Andújar, Cristina; Haouala, Rabiaa; Labidi, Nehla; Zribi, Fethia; Martı́nez, Vicente; Pérez‐Alfocea, Francisco; Abdelly, Chédly

doi:10.1016/j.jplph.2008.06.016

Cited by 42 publications

(25 citation statements)

References 52 publications

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“…As stated before, BNF is a highly energetic process which requires a constant energy supply. Modification of nodule sucrose content has been observed during drought stress (Galvez et al, 2005;Gordon et al, 1997), salt stress Lopez et al, 2008;Sanchez et al, 2011;Lopez et al, 2009;Ben Salah et al, 2009), dark stress (Gogorcena et al, 1997;Matamoros et al, 1999;Vauclare et al, 2010) and cold stress (Walsh & Layzell, 1986;van Heerden et al, 2008). However, whereas dark stress induces a diminution of sucrose concentration, www.intechopen.com drought stress, salt stress and cold stress lead in general to its accumulation in nodules.…”

Section: Stress Induced Senescence Is Characterized By Modifications mentioning

confidence: 99%

“…In contrast, the accumulation of sucrose during stress suggests that nodule glycolytic enzymes are affected. Sucrose synthase, which is involved in the degradation of sucrose into glucose and fructose in the root nodule, is inhibited during drought stress and salt stress (Ben Salah et al, 2009;Gordon et al, 1997) and malate content, which is one of the preferred substrates for bacteroid respiration (Prell and Poole, 2006), decreases under these two stress conditions (Marino et al, 2007;Galvez et al, 2005;Ben Salah et al, 2009). A fine-tuning of O 2 concentration is also important for nodule functioning, since a good supply of O 2 is determinant for nodule respiration and energy requirement while a low O 2 pressure must be maintained in the nitrogen fixing zone to prevent nitrogenase inhibition.…”

Section: Stress Induced Senescence Is Characterized By Modifications mentioning

confidence: 99%

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The Legume Root Nodule: From Symbiotic Nitrogen Fixation to Senescence

Dupont¹,

Alloing²,

Pierre³

et al. 2012

Senescence

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Section: Stress Induced Senescence Is Characterized By Modifications mentioning

confidence: 99%

Section: Stress Induced Senescence Is Characterized By Modifications mentioning

confidence: 99%

The Legume Root Nodule: From Symbiotic Nitrogen Fixation to Senescence

Dupont¹,

Alloing²,

Pierre³

et al. 2012

Senescence

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“…Here, we continue our investigation of selected lines of the legume, Medicago ciliaris, growing in symbiosis with its bacterial partner Sinorhizobium medicae. In previous studies with this species, we showed that nodules are a strong sink for assimilates and that salt tolerance of a line isolated from a Tunisian salt flat was related to its ability to conserve photosynthetic activity (Ben Salah et al 2009). We also demonstrated changes in leaf biochemical parameters between the salt-tolerant line and a salt-sensitive line (Ben Salah et al 2011).…”

Section: Introductionmentioning

confidence: 90%

Phenolic content and antioxidant activity in two contrasting Medicago ciliaris lines cultivated under salt stress

et al. 2011

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The objective of this study was to determine more indepth physiological and antioxidant responses in two Medicago ciliaris lines (a salt-tolerant line TNC 1.8 and a salt-sensitive line TNC 11.9) with contrasting responses to 100 mM NaCl. Under salt stress, both lines showed a decrease in total biomass and in the growth rate for roots, but TNC 1.8 was less affected by salt than TNC 11.9 in that it maintained leaf growth even in the presence of added salt. In both lines, salt stress mainly affected micronutrient status (Fe, Mn, Cu and Zn) rather than K nutrition, but the tolerant line TNC 1.8 accumulated more Na in leaves and less in roots compared with TNC 11.9. Salt stress decreased total soluble sugars (TSS) in all organs of the sensitive line TNC 11.9, whereas TSS was only reduced in roots of the tolerant line. The salt-induced drop in growth was linked to an increase in lipid peroxidation in roots of both lines and in leaves of the sensitive line. The salt-tolerant line TNC 1.8 was more efficient at managing salt-induced oxidative damage in leaves and to a lesser extent in roots than the salt-sensitive line TNC 11.9, by preserving higher phenolic compound and superoxide dismutase levels in both organs.

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“…SNF has been described as being sensitive to salt stress (Jebara et al 2005;López et al 2008;Ben Salah et al 2009). It has been suggested that the salt induced reduction of SNF was concomitant to a decrease in nodule sucrolytic activities, namely that of sucrose synthase (López et al 2008;Ben Salah et al 2009) causing a shortage of substrates for bacteroids respiration (Arrese-Igor et al 1999).…”

Section: Introductionmentioning

confidence: 99%

“…It has been suggested that the salt induced reduction of SNF was concomitant to a decrease in nodule sucrolytic activities, namely that of sucrose synthase (López et al 2008;Ben Salah et al 2009) causing a shortage of substrates for bacteroids respiration (Arrese-Igor et al 1999). Other studies have demonstrated that the salt induced decrease in sucrose synthase activity was associated with an increase in alkaline invertase activity that, however, did not restore SNF (Soussi et al 1999); thus, the role of sucrose synthase in the response to abiotic stresses was demonstrated (Arrese-Igor et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Salt tolerance of nitrogen fixation in Medicago ciliaris is related to nodule sucrose metabolism performance rather than antioxidant system

et al. 2010

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In this study, the effect of 100 mM NaCl on physiological and biochemical responses were investigated in nodules of two Medicago ciliaris lines differing in salt tolerance (TNC 1.8 and TNC 11.9). Results showed that, on the basis of growth and nitrogen fixation, the line TNC 1.8 proved more salt tolerant than TNC 11.9. The salt-induced oxidative stress (membrane lipid peroxidation, leghemoglobin degradation, antioxidant activities reduction) occurred similarly in nodules of both lines. The tolerant line TNC 1.8 showed a better capacity to preserve higher sucrolytic activities and maintained higher nodule malate concentration, although total organic acids decreased in both lines. The higher amount of organic acids in the tolerant line seems to be related to its capacity to maintain higher NH 4 nodule concentration in comparison with the sensitive line. Although salt stress reduced concentrations of the majority of amino acid in both lines, the decrease of the most preponderant amino acids glycine, valine, aspartate and glutamate was more accentuated in the sensitive line TNC 11.9. However, alanine concentration increased in the nodules of this sensitive line, suggesting a higher incidence of stress-induced hypoxia. The present study provides further evidence that salt tolerance of nitrogen fixation in the tolerant line is linked to a more effective supply of malate to bacteroids which allows the synthesis of amino acids required to maintain both plant and nodule growth.

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Response of nitrogen fixation in relation to nodule carbohydrate metabolism in Medicago ciliaris lines subjected to salt stress

Cited by 42 publications

References 52 publications

The Legume Root Nodule: From Symbiotic Nitrogen Fixation to Senescence

The Legume Root Nodule: From Symbiotic Nitrogen Fixation to Senescence

Phenolic content and antioxidant activity in two contrasting Medicago ciliaris lines cultivated under salt stress

Salt tolerance of nitrogen fixation in Medicago ciliaris is related to nodule sucrose metabolism performance rather than antioxidant system

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