Response of Olea europaea ssp. maderensis in vitro shoots exposed to osmotic stress

Brito, Gina; Costa, Armando; Fonseca, Henrique M.A.C.; Santos, Conceição

doi:10.1016/s0304-4238(02)00216-9

Cited by 48 publications

(23 citation statements)

References 14 publications

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“…OeTIP1.1 expression in shoot tissues is probably related to mechanisms of osmoregulation as reviewed by Luu and Maurel (2005). Salinity and drought-induced osmoregulation of olive is known in leaves and shoots (Brito et al 2003). Moreover, olive shoots are known to be very sensitive to drought, showing wide trunk diameter Xuctuations (TDF) (Jones 2004).…”

Section: Discussionmentioning

confidence: 99%

Isolation and functional characterization of three aquaporins from olive (Olea europaea L.)

Secchi

Lovisolo

Uehlein

et al. 2006

Planta

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To study the molecular bases of water transport in olive we characterized cDNAs from Olea europaea cv "Leccino" related to the aquaporin (AQP) gene family. A phylogenetic analysis of the corresponding polypeptides confirmed that they were part of water channel proteins localized in the plasma membrane and in the tonoplast. The full-length sequences were obtained by RACE-PCR and were named OePIP1.1, OePIP2.1 and OeTIP1.1. The OePIP2.1 and OeTIP1.1 encode functional water channel proteins, as indicated by expression assays in Xenopus laevis oocytes. OePIP1.1 and OePIP2.1 expression levels are high in roots and twigs and low in leaves. The highest hybridization signal of OeTIP1.1 was detected in twigs, while in roots and leaves the expression was low. To investigate the effect of abiotic stress on the transcript level of olive AQP genes, olive trees were subjected to drought treatment and the expression levels of the genes were measured by Northern-blot analysis. The transcript levels of each gene diminished strongly in plants submitted to drought stress, when soil moisture, twig water potential and twig hydraulic conductivity progressively decreased. The downregulation of AQP genes may result in reduced membrane water permeability and may limit loss of cellular water during periods of water stress. A possible role for AQPs on shoot embolism repair is discussed.

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Section: Discussionmentioning

confidence: 99%

Isolation and functional characterization of three aquaporins from olive (Olea europaea L.)

Secchi

Lovisolo

Uehlein

et al. 2006

Planta

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“…O. europaea commercial cultivars were already micropropagated from different explant sources (e.g. Rugini 1984;Canas and Benbadis 1988;Roussos and Pontikis 2002;Grigoriadou et al 2002;Brito et al 2003Zacchini and De Agazio 2004), using mostly OM medium (Rugini 1984). While for O. maderensis, an efficient plant regeneration protocol was recently achieved using a modified OM medium enriched with Fe, Mg and Mn , no information is still available for the micropropagation of O. var.…”

Section: Introductionmentioning

confidence: 99%

Assessment of genetic stability of two micropropagated wild olive species using flow cytometry and microsatellite markers

Brito

Lopes

Loureiro

et al. 2010

Trees

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Micropropagated plants from two wild-olive species, Olea maderensis and O. europaea ssp. europaea var. sylvestris were screened for genetic stability. O. maderensis shoots were elongated/multiplied on OMG medium with zeatin (9.12 lM), and rooted on 1/2 OMG with NAA (3.22 lM). O. europaea var. sylvestris shoots were elongated/multiplied on OM medium with zeatin, and rooting was optimal after a hormonal shock (IBA 100 lM) followed by transfer to the same medium without growth regulators. In both species, acclimatization was successful and plants looked normal and morphologically identical to the donor field trees. Genetic variability was assessed at several stages of the micropropagation process using flow cytometry (FCM) and nuclear microsatellites (SSR). No changes in ploidy level were found among micropropagated plants, though small deviations, putatively due to the negative effects of cytosolic compounds on propidium iodide staining, between these and field plants were observed. In SSRs analyses, ten SSR markers were able to distinguish between genotypes. No mutations were found in these tested SSR loci among the donor tree and micropropagated plants, suggesting, for the tested markers, genetic uniformity throughout the process. The FCM and SSR results obtained do not exclude the occurrence of other changes in the nuclear genome but, considering the morphological stability of micropropagated plants, indicate that both protocols are suitable and efficient for large scale, true-to-type micropropagation of these two wild olive species.

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“…Therefore, the question remains as to whether parameters exist that can be used easily, non-destructively, and with high reproducibility in rapid and/or field "diagnosis" of Cd toxicity in plants. The use of chlorophyll content and fluorescence has already been shown to be highly reliable and fairly sensitive to Cd stress in cucumis (Cucumis sativus L.) chloroplasts (Zhang et al, 2003); to other abiotic stresses such as KCl in sunflower (Helianthus annuus L.) plants (Santos et al, 2001); to osmotic stress (Brito et al, 2003); and in comparative studies of nutrient deficiencies (Ouzounidov et al, 2003). On the other hand, physiological parameters used as senescence markers may be useful in selecting Cd-tolerant plants and in assaying genetic traits.…”

Section: Introductionmentioning

confidence: 99%

Cadmium Effects on Sunflower Growth and Photosynthesis

Azevedo

Pinto

Fernandes

et al. 2005

Journal of Plant Nutrition

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The objective of this work was to evaluate the effects of cadmium (Cd) exposure on sunflower (Helianthus annuus L.) plant growth, chlorophyll content, and fluorescence. Sunflower plants were exposed to different concentrations of Cd (0, 5, 50, and 500 µM) for 21 d. Growth parameters (organ length, fresh and dry weights) were determined and results compared with two parameters associated with photosynthesis degradation: chlorophyll content and fluorescence (an easy and non-destructive method). Exposure to Cd significantly decreased growth by decreasing shoot and root lengths and their fresh and dry weight. Cadmium also decreased significantly chlorophyll content and fluorescence efficiency in all treatments. Chlorophyll a (chl a) and chl b contents showed a significant correlation with chlorophyll fluorescence (F v /F m ratio). The EC 50 values showed that the roots' length was the most sensitive endpoint in this study, followed by the roots' and shoots' weight endpoints. Also, chl b showed higher sensitivity to Cd contamination than chl a. These data show that in complement to growth parameters, the use of photosynthetic parameters provides helpful information on plant response to Cd exposure.

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Response of Olea europaea ssp. maderensis in vitro shoots exposed to osmotic stress

Cited by 48 publications

References 14 publications

Isolation and functional characterization of three aquaporins from olive (Olea europaea L.)

Isolation and functional characterization of three aquaporins from olive (Olea europaea L.)

Assessment of genetic stability of two micropropagated wild olive species using flow cytometry and microsatellite markers

Cadmium Effects on Sunflower Growth and Photosynthesis

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