Response of osteoblast-like MG63 on neoglycosylated collagen matrices

Russo, Laura; Sgambato, A; Giannoni, Paolo; Quarto, Rodolfo; Vesentini, Simone; Gautieri, Alfonso; Cipolla, Laura

doi:10.1039/c4md00056k

Cited by 8 publications

(4 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We previously showed that carbohydrate epitopes grafted to collagen are able to stimulate specific cell responses, depending on the combination of epitope and cell line [ 16 , 18 , 19 , 20 ].…”

Section: Resultsmentioning

confidence: 99%

Neoglycosylated Collagen: Effect on Neuroblastoma F-11 Cell Lines

et al. 2020

Self Cite

View full text Add to dashboard Cite

The regeneration of the nervous system is a challenging task. Currently, regenerative medicine approaches that exploit nature-inspired cues are being studied and hold great promise. The possibility to use protein-based matrices functionalized with small oligo- and monosaccharides is of interest since these can be finely tuned to better mimic the native environment. Collagen has been selected as a promising material that has the potential to be further tailored to incorporate carbohydrates in order to drive cell behavior towards neuroregeneration. Indeed, the grafting of carbohydrates to collagen 2D matrices is proved to enhance its biological significance. In the present study, collagen 2D matrices were grafted with different carbohydrate epitopes, and their potential to drive F-11 neuroblastoma cells towards neuronal differentiation was evaluated. Collagen functionalized with α-glucosides was able to differentiate neuroblastoma cells into functional neurons, while sialyl α-(2→6)-galactosides stimulated cell proliferation.

show abstract

Section: Resultsmentioning

confidence: 99%

Neoglycosylated Collagen: Effect on Neuroblastoma F-11 Cell Lines

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Both neoglycosylated Samples (3) Glc-collagen and (4) Malt-collagen were evaluated for their biocompatibility and compared with control and collagen functionalized with α-Glc by reductive amination exploiting maltose (Sample 5) (Scheme 2) [7,15].…”

Section: Resultsmentioning

confidence: 99%

A New Approach for Glyco-Functionalization of Collagen-Based Biomaterials

Figuereido

Paiotta

Magro

et al. 2019

IJMS

Self Cite

View full text Add to dashboard Cite

The cell microenvironment plays a pivotal role in mediating cell adhesion, survival, and proliferation in physiological and pathological states. The relevance of extracellular matrix (ECM) proteins in cell fate control is an important issue to take into consideration for both tissue engineering and cell biology studies. The glycosylation of ECM proteins remains, however, largely unexplored. In order to investigate the physio-pathological effects of differential ECM glycosylation, the design of affordable chemoselective methods for ECM components glycosylation is desirable. We will describe a new chemoselective glycosylation approach exploitable in aqueous media and on non-protected substrates, allowing rapid access to glyco-functionalized biomaterials.

show abstract

“…The order of reactivity can also be reversed for the attachment of reducing sugars, by exploiting the terminal aldehyde/ketone generated in the open-chain form. This strategy has been commonly exploited to mimic the glucosylation and galactosylation patterns of native collagen in ECM, via reductive amination of maltose and lactose − respectively, as well as more recently by Sgamboto et al to install sialoside epitopes able to drive stem cell fate …”

Section: Chemical Conjugationmentioning

confidence: 99%

Achieving Controlled Biomolecule–Biomaterial Conjugation

2018

View full text Add to dashboard Cite

The conjugation of biomolecules can impart materials with the bioactivity necessary to modulate specific cell behaviors. While the biological roles of particular polypeptide, oligonucleotide, and glycan structures have been extensively reviewed, along with the influence of attachment on material structure and function, the key role played by the conjugation strategy in determining activity is often overlooked. In this review, we focus on the chemistry of biomolecule conjugation and provide a comprehensive overview of the key strategies for achieving controlled biomaterial functionalization. No universal method exists to provide optimal attachment, and here we will discuss both the relative advantages and disadvantages of each technique. In doing so, we highlight the importance of carefully considering the impact and suitability of a particular technique during biomaterial design.

show abstract

Response of osteoblast-like MG63 on neoglycosylated collagen matrices

Abstract: Collagen matrices modified in order to expose galactose residues to cells were studied for their interaction with osteosarcoma-derived cell line MG63.

Cited by 8 publications

References 39 publications

Neoglycosylated Collagen: Effect on Neuroblastoma F-11 Cell Lines

Neoglycosylated Collagen: Effect on Neuroblastoma F-11 Cell Lines

A New Approach for Glyco-Functionalization of Collagen-Based Biomaterials

Achieving Controlled Biomolecule–Biomaterial Conjugation

Contact Info

Product

Resources

About