Response of proline, soluble sugars and antioxidant enzymes in wheat (Triticum aestivum L.) to different irrigation regimes in greenhouse condition

Abstract

To evaluate the response of proline and soluble sugars content, catalase (CAT) and ascorbate peroxidase (APX) activity in wheat leaves to different irrigation regimes at two growth stages, a greenhouse factorial experiment was conducted in a completely randomized design (CRD) with three replications. The factors consisted of four different irrigation regimes as 100% (I 0), 75% (I 1), 50% (I 2) and 25% (I 3) of field capacity and growth stages: 50% emergence to booting stage (GS 1) and booting stage to physiological maturity (GS 2). Fresh leaf tissues were used to determine proline and soluble sugars content, CAT and APX activity. According to the results, irrigation regimes had significant effect on proline content, soluble sugars and APX activity, but no significant differences were detected among irrigation regimes for catalase (CAT) activity. Limited irrigation increased proline concentration and total soluble sugars in leaves. None of studied traits were affected by growth stages. Interaction between irrigation regimes and growth stages was not significant for all studied traits. Results indicated that the highest proline content (12 μmol/g DW), total soluble sugars (49 mg/g DW) and APX activity (42 μmol min-1 g-1 FW) were related to irrigation at 25% of field capacity (I 3). It was concluded that proline and soluble sugar levels were increased in wheat leaves under deficit irrigation regimes.

Mild Drought Stress‐Induced Changes in Yield, Physiological Processes and Chemical Composition in Festuca, Lolium and Festulolium

“…Under drought stress conditions, increase of this component was also found in lupine, maize, cocksfoot and wheat (Volaire and Thomas , Comino et al. , Mohammadkhani and Heidari , Nazarli and Faraji ). According to many studies, reserves of sugars may contribute towards drought tolerance in plants and an increase may change the osmotic potential, which is a physiological mechanism in response to drought (Morgan , DaCosta and Huang ).…”

The effect of vermicompost leachate on morphological, physiological and biochemical indices of Stevia rebaudiana Bertoni in a soilless culture system

“…The amount of carbohydrate present in the samples was estimated using the anthrone method following the procedure used by Nazarli and Faraji (2011). Therefore, to measure the content of carbohydrate, 0.5 g of dry leaves was ground in a mortar and pestle with 5 ml of 95 % ethanol, the solution was filtered by Whatman filter paper.…”

Assessment of Deficit Irrigation Responses of Moroccan Alfalfa (Medicago Sativa L.) Landraces Grown Under Field Conditions

“…We noted strong positive correlations between K + and proline synthesis on one hand ( r = 0.876; p < 0.01) and K + and total soluble sugars in the other ( r = 0.848; p < 0.01). In addition, K + has been reported to increase the synthesis of proline, free sugars and free amino acids in rice and wheat under normal and stress conditions (Pandey et al, ; Nazarli and Faraji, ). The highest proline and K + contents noted in the more tolerant populations Adis1 and Tata2 and the lowest ones noted in the less tolerant populations Dem208 and Dem206 make it possible to suggest that these compatible solutes could be involved in the tolerance mechanism of alfalfa to water deficit through their roles as osmoregulators.…”

“…The optical density was measured at 625 nm using a spectrophotometer. The soluble sugar content was determined using a glucose standard curve and expressed as mg g −1 FW of leaves (Nazarli and Faraji, ). Three replicates per treatment per cultivar were considered with six plants each.…”