Response of Shewanella putrefaciens to low temperature regulated by membrane fluidity and fatty acid metabolism

Yang, Sheng‐Ping; Xie, Jing; Cheng, Ying; Zhang, Zhen; Zhao, Yong; Qian, Yun‐Fang

doi:10.1016/j.lwt.2019.108638

Cited by 37 publications

(36 citation statements)

References 31 publications

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“…These observations indicate that the process of UFA synthesis is induced and upregulated as a response to cold stress. Analogous results were recently shown for S. putrefaciens [ 92 ]. Interestingly, expression of the des gene (Shew185_2748) was decreased ( Supplementary Figure S3 and Table S1 ) which again correlates with the S. putrefaciens data, suggesting that the anaerobic pathway of UFA synthesis plays a major role in the cold-induced modification of membrane composition for the Shewanella strains.…”

Section: Resultssupporting

confidence: 88%

“…Moreover, increased cold adaptation is correlated with the synthesis of polyunsaturated fatty acids (PUFA), with two main genes playing an important role in this process, pks and pfaB [ 90 ]. Synthesis of UFA was described for S. oneidensis and S. putrefaciens (based on the presence of the FadR regulon and the Des enzyme), however, the PUFA synthesis was shown only for S. oneidensis and S. baltica [ 91 , 92 ]. Our transcriptome analysis revealed that the genes involved in biosynthesis of UFA and PUFA are under cold stress mediated regulation.…”

Section: Resultsmentioning

confidence: 99%

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Adaptation of the Marine Bacterium Shewanella baltica to Low Temperature Stress

Kłoska

Cech

Sadowska

et al. 2020

IJMS

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Marine bacteria display significant versatility in adaptation to variations in the environment and stress conditions, including temperature shifts. Shewanella baltica plays a major role in denitrification and bioremediation in the marine environment, but is also identified to be responsible for spoilage of ice-stored seafood. We aimed to characterize transcriptional response of S. baltica to cold stress in order to achieve a better insight into mechanisms governing its adaptation. We exposed bacterial cells to 8 °C for 90 and 180 min, and assessed changes in the bacterial transcriptome with RNA sequencing validated with the RT-qPCR method. We found that S. baltica general response to cold stress is associated with massive downregulation of gene expression, which covered about 70% of differentially expressed genes. Enrichment analysis revealed upregulation of only few pathways, including aminoacyl-tRNA biosynthesis, sulfur metabolism and the flagellar assembly process. Downregulation was observed for fatty acid degradation, amino acid metabolism and a bacterial secretion system. We found that the entire type II secretion system was transcriptionally shut down at low temperatures. We also observed transcriptional reprogramming through the induction of RpoE and repression of RpoD sigma factors to mediate the cold stress response. Our study revealed how diverse and complex the cold stress response in S. baltica is.

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Section: Resultssupporting

confidence: 88%

Section: Resultsmentioning

confidence: 99%

Adaptation of the Marine Bacterium Shewanella baltica to Low Temperature Stress

Kłoska

Cech

Sadowska

et al. 2020

IJMS

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“…First, Energy was stored by the accumulation of glycogen 16 , gluconeogenesis 17 , and compatibility solute synthesis 18 , 19 to improve the antifreeze ability of strains. Second, the up-regulation of fatty acid desaturase 20 , fatty acid metabolism regulator (FadR) and 3-hydroxy acyl-ACP dehydratase (FabA) 21 , mitogen-activated protein kinase (MAPK) signaling pathway 22 plays an important role in maintaining the normal fluidity of cell membranes at low temperatures. Third, the accumulation of polyhydroxyalkanoate (PHA) 23 and the regulation of some oxidases such as catalase, superoxide dismutase, and glutathione oxidase 24 , 25 can cope with oxidative stress caused by low temperature.…”

Section: Resultsmentioning

confidence: 99%

Complete genome sequencing of Bacillus sp. TK-2, analysis of its cold evolution adaptability

Shen

Zang²,

Sun³

et al. 2021

Sci Rep

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To date, a large number of Bacillus species from different sources have been identified. However, there are few investigations on genome information and evolutionary insights of Bacillus species from cold environments. Bacillus sp. TK-2, isolated from the soil of Changbai Mountain, is a gram-positive bacterium with cold adaptation characteristics. In this study, we present the annotated complete genome sequence of Bacillus sp. TK-2. The genome comprised 5,286,177 bp with a GC content of 35.88%, 5293 protein-encoding genes, 32 rRNA, and 77 tRNA. Numerous genes related to cold adaptation were detected in the genome of Bacillus sp. TK-2, mainly involving in energy supply, regulation of cell membrane fluidity, antioxidant, and molecular chaperones. In addition, the strain TK-2 classified in the Bacillus groups was distributed on a terminal branch with Bacillus cereus A1 by Blastn and phylogenetic analysis in NCBI database. Complete genome sequences of the strain TK-2 and Bacillus cereus A1 were compared by the online tool “Average Nucleotide Identity”, showing that the average nucleotide identity of these two strains was 98.26%. In parallel, A comparative analysis of the genomes of both Bacillus sp. TK-2 and Bacillus cereus A1 was conducted. Through the analysis of core and specific genes with cd-hit, it was found that the two strains had 5691 pan gene, 4524 core gene, and 1167 specific gene clusters. Among the 624 specific gene clusters of Bacillus sp. TK-2, some cold tolerance genes were detected, which implied the unique adaptability of Bacillus sp. TK-2 in long-term low temperature environments. Importantly, enzyme-encoding genes related to the degradation of polysaccharides such as cellulose and hemicellulose were detected in the 477 CAZyme genes of this genome. This work on sequencing and bioinformatics analysis of the complete sequence of Bacillus sp. TK-2 promote the application and in-depth research of low-temperature biotechnology.

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“…PlsY, lipB, fadR, fadI, and lpxD related to the lipid transport and metabolism were downregulated in S. putrefaciens under cold stress. FadR regulatory protein acts as a regulator controlling bacterial lipid metabolism by inhibiting the fatty acid degradation (fad) system and activating the synthesis of unsaturated fatty acids [54]. In E. coli, FadR is a key gene for the synthesis of unsaturated fatty acids and positively regulates fabA and fabB.…”

Section: Lipid Transport and Metabolismmentioning

confidence: 99%

TMT-Based Quantitative Proteomics Analysis of the Fish-Borne Spoiler Shewanella putrefaciens Subjected to Cold Stress Using LC-MS/MS

Gao

Mei

et al. 2021

Journal of Chemistry

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Shewanella putrefaciens is a specific spoilage bacterium for fish during cold storage. To better understand the molecular mechanisms of cold stress adaptation of S. putrefaciens, tandem mass tag- (TMT-) based quantitative proteomic analysis was performed to detect the effects of cold stress on protein expression profiles in S. putrefaciens which had been cultivated at 4°C and 30°C, respectively. A total of 266670 peptide spectrum matching numbers were quantified proteins after data analysis. Of the 2292 proteins quantitatively analyzed, a total of 274 were found to be differentially expressed (DE) under cold stress compared with the nonstress control. By integrating the results of Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, 9 common KEGG terms were found notable for the cold-responsive proteins. Generally, the DE proteins involved in carbohydrate, amino acid, and fatty acid biosynthesis and metabolism were significantly upregulated, leading to a specific energy conservation survival mode. The DE proteins related to DNA repair, transcription, and translation were upregulated, implicating change of gene expression and more protein biosynthesis needed in response to cold stress.

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Response of Shewanella putrefaciens to low temperature regulated by membrane fluidity and fatty acid metabolism

Cited by 37 publications

References 31 publications

Adaptation of the Marine Bacterium Shewanella baltica to Low Temperature Stress

Adaptation of the Marine Bacterium Shewanella baltica to Low Temperature Stress

Complete genome sequencing of Bacillus sp. TK-2, analysis of its cold evolution adaptability

TMT-Based Quantitative Proteomics Analysis of the Fish-Borne Spoiler Shewanella putrefaciens Subjected to Cold Stress Using LC-MS/MS

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