TMT-Based Quantitative Proteomics Analysis of the Fish-Borne Spoiler Shewanella putrefaciens Subjected to Cold Stress Using LC-MS/MS

Gao, Xin; Li, Peiyun; Mei, Jun; Xie, Jing

doi:10.1155/2021/8876986

Cited by 11 publications

(3 citation statements)

References 56 publications

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“…The technique can also be used to identify drug targets and their mechanisms of action [ 15 , 16 ]. Tandem mass tag-based (TMT) is a quantitative proteomic technique that can label and analyze multiple biological samples with high sensitivity and generate high-quality data [ 17 , 18 ]. In addition, TMT is commonly used to study the therapeutic targets and mechanisms of action of TCMs [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

TMT-based proteomics analysis of the blood enriching mechanism of the total Tannins of Gei Herba in mice

Mu,

Duan,

et al. 2024

Heliyon

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Section: Introductionmentioning

confidence: 99%

TMT-based proteomics analysis of the blood enriching mechanism of the total Tannins of Gei Herba in mice

Mu,

Duan,

et al. 2024

Heliyon

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“…coli O157:H7. Tandem mass tag (TMT) is a quantitative protein technique with in vitro isotope labeling and is one of the most powerful proteomics technologies developed recently. , Differentially expressed proteins can more comprehensively reflect the effects of AMAs on the overall metabolism in cells. In addition, multiple reaction monitoring (MRM) can simultaneously verify hundreds of target proteins.…”

Section: Introductionmentioning

confidence: 99%

TMT-Based Quantitative Proteomics Analyses Reveal the Antibacterial Mechanisms of Anthocyanins from Aronia melanocarpa against Escherichia coli O157:H7

Deng

Xue

Wang

et al. 2022

J. Agric. Food Chem.

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Aronia melanocarpa anthocyanins (AMAs), as natural plant extracts, can control pathogens and are attracting increasing attention. In this study, a tandem mass tag (TMT) quantitative proteomics method combined with multiple reaction monitoring (MRM) was used to explore the antibacterial mechanism of AMAs against Escherichia coli at the protein level. The results showed that 1739 proteins were identified in E. coli treated with AMAs, of which 628 were altered, including 262 downregulated proteins and 366 upregulated proteins. Bioinformatics analysis showed that these differentially expressed proteins have different molecular functions and participate in different molecular pathways. AMAs can affect E. coli protein biosynthesis, DNA replication and repair, oxidative stress response, peptidoglycan biosynthesis, and homeostasis. These pathways induce morphological changes and cell death. The results of this study help understand the molecular mechanism of the inhibitory effect of AMAs on food-borne pathogens and provide a reference for further development of plant-derived antimicrobial agents.

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“…Shewanella putrefaciens is a specific Gram-negative bacterium in aquatic products such as Litopenaeus vannamei ( 1 , 2 ), bigeye tuna ( 3 ), shellfish ( 4 ), and Paralichthys olivaceus ( 5 ). S. putrefaciens has strong biofilm-forming capability.…”

Section: Introductionmentioning

confidence: 99%

The enhanced expression of genes encoding diguanylate cyclases under cold stress contributes to the adhesion and biofilm formation of Shewanella putrefaciens WS13

et al. 2022

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Shewanella putrefaciens is a special spoilage bacterium of seafood during cold storage, which is easy to form biofilm and bring serious hazard to the seafood quality. Life cycle of biofilm starts after bacterial adhesion, which is essential for the formation and development of biofilm. As a ubiquitous second messenger in bacteria, c-di-GMP regulates the conversion between bacterial planktonic state and biofilm state. In this study, the adhesion and biofilm formation of S. putrefaciens WS13 under 4°C were compared to those under 30°C. Atom force microscope and scanning electron microscope were used to study the bacterial adhesion. Biofilm was analyzed by Fourier transform infrared spectroscopy, Bradford assay and phenol-sulfuric acid method. High-performance liquid chromatographic-tandem mass spectrometric and quantitative real-time PCR were applied to study c-di-GMP level and genes encoding diguanylate cyclases in cells, respectively. Results showed that the swarming mobility of S. putrefaciens WS13 was weaker under 4°C, however, the adhesive force under 4°C was 4–5 times higher than that under 30°C. Biofilm biomass, extracellular polysaccharides and extracellular proteins were 2.5 times, 3 times, and 1.6 times more than those under 30°C, respectively, but biofilm composition formed under both temperatures were similar. c-di-GMP level in S. putrefaciens WS13 under 30°C was no more than half of that in the corresponding growth stage under 4°C. Quantitative real-time PCR analysis also showed that the expression of genes encoding diguanylate cyclases were significantly enhanced under 4°C than that under 30°C. S. putrefaciens WS13 adapted to the cold stress by enhancing the expression of genes encoding diguanylate cyclases to promote bacterial adhesion and biofilm formation. This study provides a theoretical foundation for the research on the cold adaptation mechanism of specific spoilage bacteria of seafood based on c-di-GMP, and also provides a new idea to control seafood quality from the perspective of microbial molecular biology.

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TMT-Based Quantitative Proteomics Analysis of the Fish-Borne Spoiler Shewanella putrefaciens Subjected to Cold Stress Using LC-MS/MS

Cited by 11 publications

References 56 publications

TMT-based proteomics analysis of the blood enriching mechanism of the total Tannins of Gei Herba in mice

TMT-based proteomics analysis of the blood enriching mechanism of the total Tannins of Gei Herba in mice

TMT-Based Quantitative Proteomics Analyses Reveal the Antibacterial Mechanisms of Anthocyanins from Aronia melanocarpa against Escherichia coli O157:H7

The enhanced expression of genes encoding diguanylate cyclases under cold stress contributes to the adhesion and biofilm formation of Shewanella putrefaciens WS13

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