Response of the sesquiterpene synthesis in submerged cultures of the Basidiomycete <i>Tyromyces floriformis</i> to the medium composition

Grosse, Miriam; Strauss, Elisa; Krings, Ulrich; Berger, Ralf G.

doi:10.1080/00275514.2019.1668740

Cited by 11 publications

(11 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The lipophilic product yield was improved by providing a lipophilic storage phase, as the intercellular storage capacity of the cells was limited. Inspired by the work of Große et al, who achieved a tenfold increase of the desired product α-ylangene by cultivation of Tyromyces floriformis in a biphasic system [30], different oils used for wood glazing were examined as a second, lipophilic phase during the submerged cultivation. Refined linseed oil use increased the final pigment yield by 19% in comparison to the control culture, which could be used without cell disruption or solvent extraction of the biomass.…”

Section: Discussionmentioning

confidence: 99%

Pilot-Scale Production of the Natural Colorant Laetiporic Acid, Its Stability and Potential Applications

et al. 2022

Self Cite

View full text Add to dashboard Cite

Laetiporus sulphureus, a wood-decaying basidiomycete, produces yellow-orange pigments in fruiting bodies and, as was recently shown, in submerged cultivated mycelia. Out of four strains, the most potent laetiporic acid producer was identified and its yield compared in different media. The complex Moser b medium was replaced by potato dextrose broth, achieving higher yields at a lower cost. Cultivation was then scaled up from shake flask to a 7 L stirred tank bioreactor. Optimization of parameters led to increased product concentrations up to 1 g L−1, the highest yield reported so far. An in situ product recovery strategy with a biphasic system was established, increasing the yield by 19% on the shake flask scale. A crude ethanolic extract of the biomass was examined for color stability and application trials. In contrast to what has been suggested in the past, the pigment showed limited long-term stability to oxygen and light, but was stable under storage in the dark at 4 °C under nitrogen. The orange extract was successfully incorporated into different matrices like foods, cosmetics and textiles. Laetiporic acid can potentially replace petrochemical based synthetic dyes, and can thus support the development of a circular bioeconomy.

show abstract

Section: Discussionmentioning

confidence: 99%

Pilot-Scale Production of the Natural Colorant Laetiporic Acid, Its Stability and Potential Applications

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…Comparisons with mycelia were largely missing. However, a study on Tyromyces floriformis , a sesquiterpene producing basidiomycete with uncertain palatability, showed dramatic responses of submerged cultures to polysaccharides and lipids in the growth medium [ 72 ]. Rye arabinoxylan inhibited sesquiterpene synthesis, but the addition of its building blocks arabinose, xylose, or ferulic acid showed no effect, whereas only 0.05% ( w / v ) 3 2 - α -l-arabinofuranosyl-xylobiose resulted in a significant suppression.…”

Section: Effects Of the Chemical Environment On The Concentration Of ...mentioning

confidence: 99%

“…They thrive on most of the substrates shown in Table 1 by quickly inducing large enzymatic networks (own experiments, data not shown). As a result, the composition of the fruiting bodies depends on the growth substrate [ 33 , 55 , 57 , 60 , 67 , 72 , 74 ], the strain chosen [ 33 , 60 , 74 , 75 , 76 , 77 , 78 , 80 ], its developmental stage [ 29 , 30 , 31 , 32 , 38 ], and the associated adverse or beneficial microbial community [ 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 ] and is thus variable like no other food. The same applies to the mass production of fungal mycelia.…”

Section: Differences Between Fermenter Produced Fungal Mycelia and Fr...mentioning

confidence: 99%

“…The same applies to the mass production of fungal mycelia. Parameters of fungal bioprocesses can be easily manipulated, and the often-dramatic metabolic changes monitored either on-line or by off-line sampling [ 60 , 72 ]. A prototypic study on P. eryngii varied temperature, pH, energy input, carbon sources, nitrogen sources, and the C/N ratio in shake flasks and transferred the results to a 100 L batch cultivation [ 99 ].…”

Section: Differences Between Fermenter Produced Fungal Mycelia and Fr...mentioning

confidence: 99%

See 1 more Smart Citation

Mycelium vs. Fruiting Bodies of Edible Fungi—A Comparison of Metabolites

et al. 2022

Self Cite

View full text Add to dashboard Cite

Edible mushrooms are widely appreciated for their appealing flavours, low caloric values and high content of presumably health-protecting metabolites. Their long history of safe use together with the looming worldwide food crisis have revived the idea of generating meat analogues and protein isolates by the controlled fermentation of mycelia of these edible fungi as a dietary option. The occurrence of proteins, polysaccharides, smaller metabolites, metal ions and toxins in mycelia and fruiting bodies is compared among the three most popular species, Agaricus bisporus (button mushroom), Pleurotus ostreatus (oyster mushroom), Lentinus edodes (shiitake) and some closely related species. Large effects of substrate chemistry, strain, developmental stage and ecological interactions result in a wide variation of the concentrations of some metabolites in both mycelial cells and fruiting bodies. This is obviously a result of the high adaptation abilities required to survive in natural habitats. Fungal bioprocesses are decoupled from agricultural production and can be operated anytime, anywhere, and on any scale according to demand. It is concluded that fungal biomass, if produced under food-grade conditions and on an industrial scale, could provide a safe and nutritious meat substitute and protein isolates with a high biological value for future vegan foods.

show abstract

“…One experimental hurdle is presented by the low similarity of plant and fungal synthases and even between the various clades of basidiomycetes, which impedes data bank searches; another is the lack of knowledge on induction mechanisms of the coding genes. A recent report supposed that polysaccharides occurring in the natural habitat of xylophilic fungi could affect the formation of sesquiterpene synthesis [108]. Tyromyces floriformis, a potent producer of sesquiterpenes, such as α-Ylangene, was chosen as a model system.…”

Section: Formation Of Volatile Flavoursmentioning

confidence: 99%

Improved Foods Using Enzymes from Basidiomycetes

Berger

Ersoy

2022

Processes

Self Cite

View full text Add to dashboard Cite

Within the kingdom of fungi, the division Basidiomycota represents more than 30,000 species, some with huge genomes indicating great metabolic potential. The fruiting bodies of many basidiomycetes are appreciated as food (“mushrooms”). Solid-state and submerged cultivation processes have been established for many species. Specifically, xylophilic fungi secrete numerous enzymes but also form smaller metabolites along unique pathways; both groups of compounds may be of interest to the food processing industry. To stimulate further research and not aim at comprehensiveness in the broad field, this review describes some recent progress in fermentation processes and the knowledge of fungal genetics. Processes with potential for food applications based on lipases, esterases, glycosidases, peptidases and oxidoreductases are presented. The formation and degradation of colourants, the degradation of harmful food components, the formation of food ingredients and particularly of volatile and non-volatile flavours serve as examples. In summary, edible basidiomycetes are foods—and catalysts—for food applications and rich donors of genes to construct heterologous cell factories for fermentation processes. Options arise to support the worldwide trend toward greener, more eco-friendly and sustainable processes.

show abstract

Response of the sesquiterpene synthesis in submerged cultures of the Basidiomycete Tyromyces floriformis to the medium composition

Cited by 11 publications

References 36 publications

Pilot-Scale Production of the Natural Colorant Laetiporic Acid, Its Stability and Potential Applications

Pilot-Scale Production of the Natural Colorant Laetiporic Acid, Its Stability and Potential Applications

Mycelium vs. Fruiting Bodies of Edible Fungi—A Comparison of Metabolites

Improved Foods Using Enzymes from Basidiomycetes

Contact Info

Product

Resources

About