Response to Fusarium oxysporum f.sp. radicis-lycopersici in tomato roots involves regulation of SA- and ET-responsive gene expressions

Çakır, Birsen; Gül, Ayşe; Yolageldi, L.; Özaktan, Hatice

doi:10.1007/s10658-014-0394-9

Cited by 17 publications

(6 citation statements)

References 62 publications

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“…This is in contrast to other experiments, where the presence of F. oxysporum f.sp. lycopersici strains induced PAL expression as a clear evidence of induced systemic acquired resistance in tomato [ 50 ]. Symptoms on ferns and the reduction of fern FW seems to be the result of a longer period of interaction between plant and the soil-borne Fusarium spp., and it would be interesting to analyse the impact of the pathogens on aboveground parts of plants at this later stage of pathogenesis.…”

Section: Discussionmentioning

confidence: 99%

Species-Specific Impact of Fusarium Infection on the Root and Shoot Characteristics of Asparagus

et al. 2020

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Soil-borne pathogens can have considerable detrimental effects on asparagus (Asparagus officinalis) growth and production, notably caused by the Fusarium species F. oxysporum f.sp. asparagi, F. proliferatum and F. redolens. In this study, their species-specific impact regarding disease severity and root morphological traits was analysed. Additionally, various isolates were characterised based on in vitro physiological activities and on protein extracts using matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS). The response of two asparagus cultivars to the different Fusarium species was evaluated by inoculating experiments. Differences in aggressiveness were observed between Fusarium species and their isolates on roots, while no clear disease symptoms became visible in ferns eight weeks after inoculation. F. redolens isolates Fred1 and Fred2 were the most aggressive strains followed by the moderate aggressive F. proliferatum and the less and almost non-aggressive F. oxysporum isolates, based on the severity of disease symptoms. Fungal DNA in stem bases and a significant induction of pathogenesis-related gene expression was detectable in both asparagus cultivars. A significant negative impact of the pathogens on the root characteristics total root length, volume, and surface area was detected for each isolate tested, with Fred1 causing the strongest effects. No significant differences between the tested asparagus cultivars were observed.

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Section: Discussionmentioning

confidence: 99%

Species-Specific Impact of Fusarium Infection on the Root and Shoot Characteristics of Asparagus

et al. 2020

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“…Solyc09g089690 is an aminocyclopropane-1-carboxylate oxidase protein, which is induced in tomato by attack of a certain Fusarium oxysporum f.sp. radicis-lycopersici ( Çakir et al 2014 ) and is an enzyme in ethylene biosynthesis. The same InDel is present in SLKC and SLCC lines.…”

Section: Resultsmentioning

confidence: 99%

Resequencing at ≥40-Fold Depth of the Parental Genomes of a Solanum lycopersicum × S. pimpinellifolium Recombinant Inbred Line Population and Characterization of Frame-Shift InDels That Are Highly Likely to Perturb Protein Function

Kevei

King

Mohareb

et al. 2015

G3 Genes|Genomes|Genetics

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A recombinant in-bred line population derived from a cross between Solanum lycopersicum var. cerasiforme (E9) and S. pimpinellifolium (L5) has been used extensively to discover quantitative trait loci (QTL), including those that act via rootstock genotype, however, high-resolution single-nucleotide polymorphism genotyping data for this population are not yet publically available. Next-generation resequencing of parental lines allows the vast majority of polymorphisms to be characterized and used to progress from QTL to causative gene. We sequenced E9 and L5 genomes to 40- and 44-fold depth, respectively, and reads were mapped to the reference Heinz 1706 genome. In L5 there were three clear regions on chromosome 1, chromosome 4, and chromosome 8 with increased rates of polymorphism. Two other regions were highly polymorphic when we compared Heinz 1706 with both E9 and L5 on chromosome 1 and chromosome 10, suggesting that the reference sequence contains a divergent introgression in these locations. We also identified a region on chromosome 4 consistent with an introgression from S. pimpinellifolium into Heinz 1706. A large dataset of polymorphisms for the use in fine-mapping QTL in a specific tomato recombinant in-bred line population was created, including a high density of InDels validated as simple size-based polymerase chain reaction markers. By careful filtering and interpreting the SnpEff prediction tool, we have created a list of genes that are predicted to have highly perturbed protein functions in the E9 and L5 parental lines.

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“…Fungal pathogens F. oxysporum [ 26 , 27 ], V. dahliae , and F. solani were quantified based on disease severity [ 28 ] and classified into five levels according to observations during pathogen invasion: (0) healthy plants (without obvious wilting or yellowing symptoms); (1) Cotyledon wilt or fall off; (2) plants with 30–50% true leaves withered or shed; (3) plants with 50–80% true leaves withered or shed; and (4) plants with all leaves shed or dead. The disease classification was scored 14 days after pathogen infection, using five individual plants for each treatment.…”

Section: Methodsmentioning

confidence: 99%

Tomato-Thaumatin-like Protein Genes Solyc08g080660 and Solyc08g080670 Confer Resistance to Five Soil-Borne Diseases by Enhancing β-1,3-Glucanase Activity

et al. 2023

Genes

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Although thaumatin-like proteins (TLPs) are involved in resistance to a variety of fungal diseases, whether the TLP5 and TLP6 genes in tomato plants (Solanum lycopersicum) confer resistance to the pathogenesis of soil-borne diseases has not been demonstrated. In this study, five soil-borne diseases (fungal pathogens: Fusarium solani, Fusarium oxysporum, and Verticillium dahliae; bacterial pathogens: Clavibacter michiganense subsp. michiganense and Ralstonia solanacearum) were used to infect susceptible “No. 5” and disease-resistant “S-55” tomato cultivars. We found that SlTLP5 and SlTLP6 transcript levels were higher in susceptible cultivars treated with the three fungal pathogens than in those treated with the two bacterial pathogens and that transcript levels varied depending on the pathogen. Moreover, the SlTLP5 and SlTLP6 transcript levels were much higher in disease-resistant cultivars than in disease-susceptible cultivars, and the SlTLP5 and SlTLP6 transcript levels were higher in cultivars treated with the same fungal pathogen than in those treated with bacterial pathogens. SlTLP6 transcript levels were higher than SlTLP5. SlTLP5 and SlTLP6 overexpression and gene-edited transgenic mutants were generated in both susceptible and resistant cultivars. Overexpression and knockout increased and decreased resistance to the five diseases, respectively. Transgenic plants overexpressing SlTLP5 and SlTLP6 inhibited the activities of peroxidase (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT) after inoculation with fungal pathogens, and the activities of POD, SOD, and APX were similar to those of fungi after infection with bacterial pathogens. The activities of CAT were increased, and the activity of β-1,3-glucanase was increased in both the fungal and bacterial treatments. Overexpressed plants were more resistant than the control plants. After SlTLP5 and SlTLP6 knockout plants were inoculated, POD, SOD, and APX had no significant changes, but CAT activity increased and decreased significantly after the fungal and bacterial treatments, contrary to overexpression. The activity of β-1,3-glucanase decreased in the treatment of the five pathogens, and the knocked-out plants were more susceptible to disease than the control. In summary, this study contributes to the further understanding of TLP disease resistance mechanisms in tomato plants.

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Response to Fusarium oxysporum f.sp. radicis-lycopersici in tomato roots involves regulation of SA- and ET-responsive gene expressions

Cited by 17 publications

References 62 publications

Species-Specific Impact of Fusarium Infection on the Root and Shoot Characteristics of Asparagus

Species-Specific Impact of Fusarium Infection on the Root and Shoot Characteristics of Asparagus

Resequencing at ≥40-Fold Depth of the Parental Genomes of a Solanum lycopersicum × S. pimpinellifolium Recombinant Inbred Line Population and Characterization of Frame-Shift InDels That Are Highly Likely to Perturb Protein Function

Tomato-Thaumatin-like Protein Genes Solyc08g080660 and Solyc08g080670 Confer Resistance to Five Soil-Borne Diseases by Enhancing β-1,3-Glucanase Activity

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