Response to Prof. Y. Shen (SC2)

Liu, Hu

doi:10.5194/hess-2018-518-ac1

Cited by 52 publications

(87 citation statements)

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“…Similarly, GA has been reported to suppress colon cancer cell proliferation, migration, and invasion and to inhibit the epithelial to mesenchymal transition in lung cancer cells through repression of the mTOR signaling pathway, which is the major negative regulator of autophagy. 20,53 Furthermore, we observed increased colocalization of mitochondria and autophagosomes/lysosomes. Additionally, autophagy inhibition induced chemically or genetically can restore the GA-induced mitochondrial mass loss.…”

Section: Discussionmentioning

confidence: 76%

Ginkgolic Acids Impair Mitochondrial Function by Decreasing Mitochondrial Biogenesis and Promoting FUNDC1-Dependent Mitophagy

Wang¹,

Wang²,

Ruan³

et al. 2019

J. Agric. Food Chem.

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Ginkgolic acids (GAs) are found in the leaves, nuts, and testa of Ginkgo biloba and have been reported to exhibit antitumor, antibacterial, and pro-apoptotic activities. However, their role in mitochondrial function is still unclear. Our previous study showed that genes related to the mitochondria present significant changes in GA-treated mouse bone marrow stromal cells. We hypothesize that GAs may regulate mitochondrial function. Here, we found that GA treatment induced mitochondrial fragmentation, reduced mtDNA copy numbers and mitochondrial protein levels, and impaired mitochondrial adenosine 5′-triphosphate production and oxygen consumption. The GA-induced mitochondrial mass loss may be due to decreased mitochondrial biogenesis. In addition, abolishing autophagy by Atg7 knockout or the administration of an autophagy inhibitor can restore the GA-induced decrease in mitochondrial mass. Furthermore, FUNDC1 knockdown restored the GA-induced changes in mitochondrial mass reduction and mitochondrial membrane potential loss. Together, our studies demonstrated that GAs impaired mitochondrial function by decreasing mitochondrial biogenesis and promoting FUNDC1dependent mitophagy.

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Section: Discussionmentioning

confidence: 76%

Ginkgolic Acids Impair Mitochondrial Function by Decreasing Mitochondrial Biogenesis and Promoting FUNDC1-Dependent Mitophagy

Wang¹,

Wang²,

Ruan³

et al. 2019

J. Agric. Food Chem.

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“…Although further investigation is necessary, the significantly enhanced hydrogel adhesion demonstrated in these experiments may be attributed to covalent bonding of amine and thiol groups in the collagen hydrogel with PDA through Michael addition and Schiff base formation. 27,28 PDA can also engage in noncovalent interactions with the ECM hydrogel through hydrogen bonding, cation−π, π−π, and electrostatic interactions, 29,30 which can further increase the strength of interfacial adhesion. The hydrophilicity of PDA-treated PDMS may be another contributing factor, as increased surface wettability can facilitate the interaction of highly hydrated ECM scaffolds with the surface of the culture chamber.…”

Section: Resultsmentioning

confidence: 99%

Polydopamine-Based Interfacial Engineering of Extracellular Matrix Hydrogels for the Construction and Long-Term Maintenance of Living Three-Dimensional Tissues

Park

Georgescu

et al. 2019

ACS Appl. Mater. Interfaces

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Diverse biological processes in the body rely on the ability of cells to exert contractile forces on their extracellular matrix (ECM). In three-dimensional (3D) cell culture, however, this intrinsic cellular property can cause unregulated contraction of ECM hydrogel scaffolds, leading to a loss of surface anchorage and the resultant structural failure of in vitro tissue constructs. Despite advances in the 3D culture technology, this issue remains a significant challenge in the development and long-term maintenance of physiological 3D in vitro models. Here, we present a simple yet highly effective and accessible solution to this problem. We leveraged a single-step surface functionalization technique based on polydopamine to drastically increase the strength of adhesion between hydrogel scaffolds and cell culture substrates. Our method is compatible with different types of ECM and polymeric surfaces and also permits prolonged shelf storage of functionalized culture substrates. The proof-of-principle of this technique was demonstrated by the stable long-term (1 month) 3D culture of human lung fibroblasts. Furthermore, we showed the robustness and advanced application of the method by constructing a dynamic cell stretching system and performing over 100 000 cycles of mechanical loading on 3D multicellular constructs for visualization and quantitative analysis of stretch-induced tissue alignment. Finally, we demonstrated the potential of our technique for the development of microphysiological in vitro models by establishing microfluidic 3D co-culture of vascular endothelial cells and fibroblasts to engineer self-assembled, perfusable 3D microvascular beds.

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“…For a further study of the ZnO/Ag/rGO ternary composites, the representative ternary composite (ZRA 1-2) was analyzed using XPS. The C/O value of the ZnO/Ag/rGO composite was 3.33, which is significantly higher than that of GO (1.38) [38][39][40]. The decrease in the C/O value suggests the partial reduction of GO to rGO, which maintains the dispersivity and stability of the ZnO/Ag/rGO composite.…”

Section: Formation Of Zno/ag/rgo Compositesmentioning

confidence: 90%

Light-induced ZnO/Ag/rGO bactericidal photocatalyst with synergistic effect of sustained release of silver ions and enhanced reactive oxygen species

Zhang

Zhou

et al. 2019

Chinese Journal of Catalysis

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Response to Prof. Y. Shen (SC2)

Cited by 52 publications

References 0 publications

Ginkgolic Acids Impair Mitochondrial Function by Decreasing Mitochondrial Biogenesis and Promoting FUNDC1-Dependent Mitophagy

Ginkgolic Acids Impair Mitochondrial Function by Decreasing Mitochondrial Biogenesis and Promoting FUNDC1-Dependent Mitophagy

Polydopamine-Based Interfacial Engineering of Extracellular Matrix Hydrogels for the Construction and Long-Term Maintenance of Living Three-Dimensional Tissues

Light-induced ZnO/Ag/rGO bactericidal photocatalyst with synergistic effect of sustained release of silver ions and enhanced reactive oxygen species

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