Response to the Report by Drexler Et Al

Suda, Kayoshi; Rothen‐Rutishauser, Barbara; Günthert, Maja; Wunderli‐Allenspach, Heidi

doi:10.1290/1071-2690(2002)038<0187:rttrbd>2.0.co;2

Cited by 4 publications

(5 citation statements)

References 4 publications

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“…Immunohistochemical staining with pancytokeratin antibody was performed to characterize cultured keratinocytes (Suda et al 2001). Similar to fibroblast characterization, keratinocytes cultured on the cover slide were fixed and permeabilized, and then incubated with mouse anti-pan-cytokeratin antibody (1:200 in PBS, Sigma) at 4°C overnight followed by incubating with Alexa Floura-555 conjugated goat anti-mouse IgG antibody (1:1000 in PBS, Invitrogen) at room temperature for 30 min.…”

Section: Cell Culturementioning

confidence: 99%

Compressed collagen gel as the scaffold for skin engineering

Shi

Zhu

et al. 2010

Biomed Microdevices

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Collagen gel scaffolds can potentially be utilized as cell seeded systems for skin tissue engineering. However, its dramatic contraction after being mixed with cells and its mechanical weakness are the drawbacks for its application to skin engineering. In this study, a compressed collagen gel scaffold was fabricated through the rapid expulsion of liquid from reconstituted gels by the application of 'plastic compression'(PC) technique. Both compressed and uncompressed gels were characterized with their gel contraction rate, morphology, the viability of seeded cells, their mechanical properties and the feasibility as a scaffold for constructing tissue-engineered skin. The results showed that the compression could significantly reduce the contraction of the collagen gel and improve its mechanical property. In addition, seeded dermal fibroblasts survived well in the compressed gel and seeded epidermal cells gradually developed into a stratified epidermal layer, and thus formed tissue engineered skin. This study reveals the potential of using compressed collagen gel as a scaffold for skin engineering.

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Section: Cell Culturementioning

confidence: 99%

Compressed collagen gel as the scaffold for skin engineering

Shi

Zhu

et al. 2010

Biomed Microdevices

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“…DNA fingerprint analysis of ECV304 cells has indicated that they are genotypically identical to T24/83 epithelial cells [13]. However, this technique has limitations and we and others [10,14,15] have shown that functional and morphological differences between ECV304 and T24/83 cells do exist. ECV304 cells display many endothelial cell markers and features, some of which, (e.g.…”

Section: Discussionmentioning

confidence: 96%

“…ECV304 cells display many endothelial cell markers and features, some of which, (e.g. Von Willebrand factor, uptake of low-density lipoprotein and vimentin) are not expressed by T24/83 cells [14,15]. Unlike T24/83, EaHy929, b.End5 and primary rat brain endothelial cells, ECV304 cells respond to astrocyte influence with an enhanced transendothelial electrical resistance [10].…”

Section: Discussionmentioning

confidence: 99%

“…Unlike T24/83, EaHy929, b.End5 and primary rat brain endothelial cells, ECV304 cells respond to astrocyte influence with an enhanced transendothelial electrical resistance [10]. With the demonstration that the T24/83 cellular tight junction network is poorly developed compared with that of ECV304 cells [15], it is apparent that the two cell types are not functionally identical. Given that the ECV304 cell line displays endothelial features, is the only cell line to demonstrate a developed tight junction network, and responds to astrocyte influence, its use as a model system of the blood-brain barrier is therefore warranted.…”

Section: Discussionmentioning

confidence: 99%

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Activated T cells mediate direct blood–brain barrier endothelial cell death and dysfunction

Tan

Purcell²,

Heales³

et al. 2002

NeuroReport

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Neuro-inflammation is characterized by immune cell infiltration across the blood-brain barrier, a process instrumental in neuronal cell death. In neuro-inflammation the blood-brain barrier is also damaged and the consequences of activated lymphocytes on the integrity of the blood-brain barrier is not well characterized. Utilizing an blood-brain barrier model we demonstrate that endothelial cell viability and barrier integrity are directly altered following lymphocyte exposure. The effect of activated lymphocytes is cell number dependent, mostly mediated by direct contact, and is not associated with the pro-inflammatory cytokine TNF-alpha. For the successful treatment of neuro-inflammatory disease, intervention of this direct effect at the blood-brain barrier is warranted.

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“…DNA fingerprinting at DSMZ (German Collection of Microorganisms and Cell Cultures) and STR-PCR (Short tandem repeat PCR) test at JCRB (Japanese Collection of Research Bioresources) showed that the cell line ECV304 is a derivative of human urinary bladder carcinoma cell line T-24 [28,29]. However, the phenotypic characterization of this cell line showed that several endothelial markers (von Willebrand factor, uptake of low-density lipoprotein, vimentin) could clearly be identified in ECV304, but not in T24 cells [30]. Although there are apparent discrepancies between the genotype and phenotype in this cell line, ECV304 cells function as an endothelial cell line in the investigation of vascular biology was highly documented in the past decade [31,32,33,34,35].…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Proliferation of ECV304 Cells by a Disintegrin from Chinese Green Tree Viper

Han¹,

Lu²,

Xu³

et al. 2007

PPL

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A novel disintegrin, stejnin, was purified from the Trimeresurus stejnegeri venom by gel filtration and reverse phase high performance liquid chromatography. The molecular weight of stejnin was determined to be 7428 Da by MALD-TOF MS analysis. The cDNA encoding the precursor of stejnin was cloned from the venom gland. From the deduced amino acid sequence, stejnin is composed of 71 amino acid residues contains the tripeptide sequence Arg-Gly-Asp (RGD), a well-known characteristic of the disintegrin family. Stejnin strongly inhibited ADP- and ristomycin-induced human platelet aggregation with IC50 of 45 and 50 nM, respectively. Stejnin also possessed potent inhibited cell proliferation of ECV304 cells.

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Response to the Report by Drexler Et Al

Cited by 4 publications

References 4 publications

Compressed collagen gel as the scaffold for skin engineering

Compressed collagen gel as the scaffold for skin engineering

Activated T cells mediate direct blood–brain barrier endothelial cell death and dysfunction

Inhibition of Proliferation of ECV304 Cells by a Disintegrin from Chinese Green Tree Viper

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