Responses of ABCB and ABCC transporters to the toxic dinoflagellate Prorocentrum lima in the mussel Perna viridis

Lv, Jin-Jin; Yuan, Kuan-Kuan; Lu, Guan-Xiu; Li, Hongye; Kwok, Hang Fai; Yang, Wei‐Dong

doi:10.1016/j.aquatox.2022.106368

Cited by 8 publications

(2 citation statements)

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“…It remains unclear whether esterification plays a key role in the tolerance of P. viridis to DSTs. In line with our previous results [ 42 ], here we also found that the concentration of total DSTs (DSTs + DST ester) was significantly higher than that of free DSTs in the digestive gland of the mussel 96 h after exposure to P. lima ( Figure 1 ), indicating the presence of esterified DSTs in the mussel.…”

Section: Discussionsupporting

confidence: 93%

“…Multi-reaction monitoring mode (MRM) for negative ionization was used to detect OA and DTX1. As described in our previous study [ 42 ], the standards of OA (LC Laboratories, Woburn, MA, USA) and DTX1 (NRC, CAN), were diluted, and standard curves of chromatographic peak area (y) for OA and DTX1 against the toxin concentration (x) were established.…”

Section: Methodsmentioning

confidence: 99%

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Possible Role of Docosahexaenoic Acid in Response to Diarrhetic Shellfish Toxins in the Mussel Perna viridis

et al. 2023

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Marine bivalves are rich in docosahexaenoic acid (DHA), a polyunsaturated fatty acid known to be beneficial for human health; however, the potential role of DHA in protecting shellfish from the toxicity of diarrhetic shellfish toxins (DSTs) remains poorly understood. Here, we aimed to study the effect of DHA on the response of the bivalve, Perna viridis, to DSTs by using LC-MS/MS, RT-qPCR, and histological examination. In this study, we observed that the DHA content decreased significantly with esterification of DSTs in the digestive gland of the mussel P. viridis after 96 h of exposure to Prorocentrum lima, a DST-producing dinoflagellate. The addition of DHA significantly increased the esterification level of DSTs and increased the expression of Nrf2 signaling pathway-related genes and enzyme activities, alleviating the damage of DSTs to digestive glands. These results suggested that DHA may mediate the esterification of DSTs and activation of the Nrf2 signaling pathway in P. viridis to protect mussels from the toxic effects of DSTs. This study may provide new insights regarding the response of bivalves to DSTs and lay the foundation for uncovering the role of DHA in environmental adaptation of bivalves.

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Section: Discussionsupporting

confidence: 93%

Section: Methodsmentioning

confidence: 99%