Responses of barley root and shoot proteomes to long‐term nitrogen deficiency, short‐term nitrogen starvation and ammonium

Møller, Anders Hauer; Pedas, Pai; Andersen, B. N.; Svensson, Birte; Schjøerring, Jan K.; Finnie, Christine

doi:10.1111/j.1365-3040.2011.02396.x

Cited by 67 publications

(57 citation statements)

References 75 publications

(156 reference statements)

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“…Glutamine synthetase (GS) is an enzyme that plays an essential role in N metabolism by catalyzing the ATP‐dependent conversion of glutamate and ammonia to form glutamine. Previous studies have showed that N and P deficiencies cause a decrease in the abundance of GS in barley and maize . In this study, the abundances of both GS1 and GS2 were down‐regulated in N‐ and P‐deficient females but not in males, suggesting that long‐term N and P deficiencies may affect nitrogen assimilation more in females than in males.…”

Section: Discussionmentioning

confidence: 42%

iTRAQ-based quantitative proteomic analysis gives insight into sexually different metabolic processes of poplars under nitrogen and phosphorus deficiencies

et al. 2016

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Male and female poplars (Populus cathayana Rehd.) respond differently to nitrogen (N) and phosphorus (P) deficiencies. In this study, an iTRAQ-based quantitative proteomic analysis was performed. N and P deficiencies caused 189 and 144 proteins to change in abundance in males and 244 and 464 in females, respectively. Compared to N- and P-deficient males, both N- and P-deficient females showed a wider range of changes in proteins that are involved in amino acid, carbohydrate and protein metabolism, and the sexual differences were significant. When comparing the effects of N- and P-deficiencies, N-deficient females expressed more changes in proteins that are involved in stress responses and gene expression regulation, while P-deficient females showed more changes in proteins that are involved in energy and lipid metabolism, stress responses and gene expression regulation. The quantitative RT-PCR analysis of stress-related proteins showed that males have a better expression correlation between mRNA and protein levels than do females. This study shows that P. cathayana females are more sensitive and have more rapid metabolic mechanisms when responding to N and P deficiencies than do males, and P deficiency has a wider range of effects on females than does N deficiency.

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Section: Discussionmentioning

confidence: 42%

iTRAQ-based quantitative proteomic analysis gives insight into sexually different metabolic processes of poplars under nitrogen and phosphorus deficiencies

et al. 2016

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“…An increase and decrease in chloroplast RNA binding protein was observed in salt-tolerant and salt-sensitive barley genotypes, respectively (Fatehi et al 2012a). Two chloroplast RNA-binding proteins, Cp31AHv and Cp31BHv, increased in abundance, and one of these (Cp31BHv) also increased under long-term nitrogen deficiency in salt-tolerant H. vulgare L. cultivar (Møller et al 2011).…”

Section: Protein Translation and Processingmentioning

confidence: 95%

The proteome response of “Hordeum marinum” to long-term salinity stress

Boustani

Fatehi

Azizinezhad

2017

Cereal Research Communications

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Salinity is a major constraint to crop productivity and mechanisms of plant responses to salinity stress are extremely complex. "Hordeum marinum" is a salt tolerant barley species, which could be a good source to evaluate salt-tolerance patterns. Proteomics is a powerful technique to identify proteins involved in plant adaptation to stresses. We applied a proteomic approach to better understanding the mechanism of plant responses to salinity in a salt-tolerant genotype of barley. At the 4-leaf stage, plants were exposed to 0 (control treatment) or 300 mM NaCl (salt treatment). Salt treatment was maintained for 3 weeks. Total proteins of leaf 4 were extracted and separated by two-dimensional gel electrophoresis. More than 290 protein spots were reproducibly detected. Of these, 20 spots showed significant changes to salt treatment compared to the control: 19 spots were upregulated and 1 spot was absent. Using MALDI-TOF/TOF MS, we identified 20 cellular proteins which represented 11 different proteins and were classified into five categories. These proteins were involved in various cellular functions. Upregulation of proteins which involved in protein processing (ribosomal protein, cullin family, cp31AHv protein and RNA recognition motif (RRM) superfamily), photosynthesis (Ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) and Ribulose bisphosphate carboxylase/oxygenase activase (rubisco activase)), energy metabolism (cytosolic malate dehydrogenase (cyMDH) and fructokinase), oxygen species scavenging and defense (cystatin and thioredoxin) may increase plant adaptation to salt stress.

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“…From a physiological perspective, nitrate nutrition is associated with elevated cytokinin biosynthesis, increased organic acid content, and soil alkalinization, while ammonium nutrition is associated with increased activity of alternative respiratory pathways, elevated amino acid content, and soil acidification (Walch-Liu et al, 2005;Patterson et al, 2010). In addition, recent studies have demonstrated distinct transcriptome, proteome, and protein phosphorylation signatures in plants supplied with either ammonium or nitrate as a nitrogen source (Patterson et al, 2010;Møller et al, 2011;Engelsberger and Schulze, 2012).…”

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confidence: 99%

Nitrate-Regulated Glutaredoxins Control Arabidopsis Primary Root Growth

et al. 2015

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Nitrogen is an essential soil nutrient for plants, and lack of nitrogen commonly limits plant growth. Soil nitrogen is typically available to plants in two inorganic forms: nitrate and ammonium. To better understand how nitrate and ammonium differentially affect plant metabolism and development, we performed transcriptional profiling of the shoots of ammoniumsupplied and nitrate-supplied Arabidopsis (Arabidopsis thaliana) plants. Seven genes encoding class III glutaredoxins were found to be strongly and specifically induced by nitrate. RNA silencing of four of these glutaredoxin genes (AtGRXS3/4/5/8) resulted in plants with increased primary root length (approximately 25% longer than the wild type) and decreased sensitivity to nitratemediated inhibition of primary root growth. Increased primary root growth is also a well-characterized phenotype of many cytokinin-deficient plant lines. We determined that nitrate induction of glutaredoxin gene expression was dependent upon cytokinin signaling and that cytokinins could activate glutaredoxin gene expression independent of plant nitrate status. In addition, crosses between "long-root" cytokinin-deficient plants and "long-root" glutaredoxin-silenced plants generated hybrids that displayed no further increase in primary root length (i.e. epistasis). Collectively, these findings suggest that AtGRXS3/4/5/8 operate downstream of cytokinins in a signal transduction pathway that negatively regulates plant primary root growth in response to nitrate. This pathway could allow Arabidopsis to actively discriminate between different nitrogen sources in the soil, with the preferred nitrogen source, nitrate, acting to suppress primary root growth (vertical dimension) in concert with its well-characterized stimulatory effect on lateral root growth (horizontal dimension).

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Responses of barley root and shoot proteomes to long‐term nitrogen deficiency, short‐term nitrogen starvation and ammonium

Abstract: Cereals are major crops worldwide, and improvement of their nitrogen use efficiency is a crucial challenge. In this study proteins responding to N supply in barley roots and shoots were analysed using a proteomics approach, to provide insight into mechanisms of N uptake and assimila-

Cited by 67 publications

References 75 publications

iTRAQ-based quantitative proteomic analysis gives insight into sexually different metabolic processes of poplars under nitrogen and phosphorus deficiencies

iTRAQ-based quantitative proteomic analysis gives insight into sexually different metabolic processes of poplars under nitrogen and phosphorus deficiencies

The proteome response of “Hordeum marinum” to long-term salinity stress

Nitrate-Regulated Glutaredoxins Control Arabidopsis Primary Root Growth

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